干旱区生态环境知识资源中心(Arid): 干旱区1株新欧文氏属菌的分离及溶磷特性

Arid

	干旱区1株新欧文氏属菌的分离及溶磷特性
其他题名	Isolation and Phosphate-solubilizing Characteristics of a Novel Erwinia sp. Bacterium in Arid Environment
	侯娇; 李莎
来源期刊	环境科学与技术
ISSN	1003-6504
出版年	2021
卷号	44 期号:2 页码:1003-6504
中文摘要	为开发适于使用的微生物磷肥,该研究从库布齐沙漠根际土中筛选了1株高效溶磷细菌,命名为GY35。根据菌株的16S rRNA基因分析和生理生化特征,将菌株GY35鉴定为欧文氏属(Erwinia)细菌。研究表明,菌株GY35的溶磷能力与其产生葡萄糖酸导致pH降低呈正相关。在NBRIP培养基中按1%的量接种GY35,24 h后培养基中的葡萄糖酸浓度升高到33.5 mg/L、pH降低至3.5,可溶性磷浓度达到363.9 mg/L,菌体数量达1.0*10~9 CFU/mL。菌株GY35溶磷的最适温度为30 ℃,最适pH值为6.5~7.0,在碱性条件下的溶磷效率高于酸性条件。接种菌株GY35后,土壤中速效磷含量提高了15.1%,植物产量提高了30.7%。
英文摘要	In order to develop suitable microbial phosphate fertilizer, a high efficient phosphate-solubilizing bacterium named GY35 was screened from the rhizosphere soil of Kubuqi desert. According to the 16S rRNA gene analysis and the physiological and biochemical characteristics, the strain GY35 was identified as Erwinia sp. bacterium. Studies showed that the phosphate solubilizing ability of strain GY35 was positively correlated with the pH reduction caused by D-gluconic acid production. When GY35 was inoculated in the NBRIP medium at the concentration of 1%, the concentration of D-gluconic acid increased to 33.5 mg/L, pH decreased to 3.5, the concentration of soluble phosphorus reached 363.9 mg/L, and the num- ber of mycelium reached 1.0*10~9 CFU/mL after 24 hours. The optimal temperature and pH value of the strain GY35 were 30 ℃ and 6.5~7.0 respectively. The phosphorus dissolving efficiency of strain GY35 was higher in alkaline condition than in acid condition. After inoculation with strain GY35, the content of available phosphorus in soil increased by 15.1%, and the plant yield increased by 30.7%.
中文关键词	溶磷菌 ; 欧文氏菌 ; 溶磷特性 ; 土壤养分
英文关键词	phosphate-solubilizing bacteria Erwinia sp. phosphate-solubilizing characteristics soil nutrient
类型	Article
语种	中文
国家	中国
收录类别	CSCD
WOS类目	Environmental Sciences & Ecology
CSCD记录号	CSCD:6968363
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/365132
作者单位	侯娇, 重庆大学城市科技学院, 重庆 402160, 中国.; 李莎, 重庆大学城市科技学院, 重庆 402160, 中国.
推荐引用方式 GB/T 7714	侯娇,李莎. 干旱区1株新欧文氏属菌的分离及溶磷特性[J],2021,44(2):1003-6504.
APA	侯娇,&李莎.(2021).干旱区1株新欧文氏属菌的分离及溶磷特性.环境科学与技术,44(2),1003-6504.
MLA	侯娇,et al."干旱区1株新欧文氏属菌的分离及溶磷特性".环境科学与技术 44.2(2021):1003-6504.