干旱区生态环境知识资源中心(Arid): Single-Cell RNA Sequencing Reveals a Unique Monocyte Population in Bronchoalveolar Lavage Cells of Mice Challenged With Afghanistan Particulate Matter and Allergen

Arid

DOI	10.1093/toxsci/kfab065
	Single-Cell RNA Sequencing Reveals a Unique Monocyte Population in Bronchoalveolar Lavage Cells of Mice Challenged With Afghanistan Particulate Matter and Allergen
	Berman, Reena; Min, Elysia; Huang, Jie; Kopf, Katrina; Downey, Gregory P.; Riemondy, Kent; Smith, Harry A.; Rose, Cecile S.; Seibold, Max A.; Chu, Hong Wei; Day, Brian J.
通讯作者	Chu, HW ; Day, BJ (corresponding author), Natl Jewish Hlth, Dept Med, 1400 Jackson St, Denver, CO 80206 USA.
来源期刊	TOXICOLOGICAL SCIENCES
ISSN	1096-6080
EISSN	1096-0929
出版年	2021
卷号	182 期号:2 页码:297-309
英文摘要	Upon returning from deployment to Afghanistan, a substantial number of U.S. military personnel report deploymentrelated lung disease (DRLD) symptoms, including those consistent with an asthma-like airways disease. DRLD is thought to be caused by prolonged inhalation of toxic desert particulate matter, which can persist in the postdeployment setting such as exposure to common household allergens. The goal of this study was to define the transcriptomic responses of lung leukocytes of mice exposed to Afghanistan desert particulate matter (APM) and house dust mite (HDM). C57BL/6 mice (n = 15/group) were exposed to filtered air or aerosolized APM for 12 days, followed by intranasal PBS or HDM allergen challenges for 24 h. Bronchoalveolar lavage (BAL) cells were collected for single-cell RNA sequencing (scRNAseq), and assessment of inflammation and airway hyper-responsiveness. Unsupervised clustering of BAL cell scRNAseq data revealed a unique monocyte population induced only by both APM and allergen treatments. This population of monocytes is characterized by the expression of genes involved in allergic asthma, including Alox15. We validated Alox15 expression in monocytes via immunostaining of lung tissue. APM pre-exposure, followed by the HDM challenge, led to significantly increased total respiratory system resistance compared with filtered air controls. Using this mouse model to mimic DRLD, we demonstrated that inhalation of airborne PM during deployment may prime airways to be more responsive to allergen exposure after returning home, which may be linked to dysregulated immune responses such as induction of a unique lung monocyte population.
英文关键词	particulate matter allergic asthma ALOX15 single-cell RNA sequencing
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000692636900011
WOS关键词	SOUTHWEST ASIA ; INFLAMMATION ; MILITARY ; ACID ; EXPRESSION ; EXPOSURE ; HEALTH
WOS类目	Toxicology
WOS研究方向	Toxicology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/364797
作者单位	[Berman, Reena; Min, Elysia; Huang, Jie; Kopf, Katrina; Downey, Gregory P.; Rose, Cecile S.; Chu, Hong Wei; Day, Brian J.] Natl Jewish Hlth, Dept Med, 1400 Jackson St, Denver, CO 80206 USA; [Riemondy, Kent; Smith, Harry A.] Univ Colorado, RNA Biosci Initiat, Sch Med, Aurora, CO 80045 USA; [Seibold, Max A.] Natl Jewish Hlth, Ctr Genes Environm & Hlth, Denver, CO 80206 USA
推荐引用方式 GB/T 7714	Berman, Reena,Min, Elysia,Huang, Jie,et al. Single-Cell RNA Sequencing Reveals a Unique Monocyte Population in Bronchoalveolar Lavage Cells of Mice Challenged With Afghanistan Particulate Matter and Allergen[J],2021,182(2):297-309.
APA	Berman, Reena.,Min, Elysia.,Huang, Jie.,Kopf, Katrina.,Downey, Gregory P..,...&Day, Brian J..(2021).Single-Cell RNA Sequencing Reveals a Unique Monocyte Population in Bronchoalveolar Lavage Cells of Mice Challenged With Afghanistan Particulate Matter and Allergen.TOXICOLOGICAL SCIENCES,182(2),297-309.
MLA	Berman, Reena,et al."Single-Cell RNA Sequencing Reveals a Unique Monocyte Population in Bronchoalveolar Lavage Cells of Mice Challenged With Afghanistan Particulate Matter and Allergen".TOXICOLOGICAL SCIENCES 182.2(2021):297-309.