干旱区生态环境知识资源中心(Arid): A UHPLC/MS/MS method for the analysis of active and inactive forms of GLP-1 and GIP incretins in human plasma

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DOI	10.1016/j.talanta.2021.122806
	A UHPLC/MS/MS method for the analysis of active and inactive forms of GLP-1 and GIP incretins in human plasma
	Villano, Debora; Zafrilla, Pilar; Garcia-Viguera, Cristina; Dominguez-Perles, Raul
通讯作者	Villano, D (corresponding author), Univ Catolica San Antonio Murcia UCAM, Fac Hlth Sci, Sch Pharm, Res Grp Nutr & Oxidat Stress, Campus Jeronimos, Murcia 30107, Spain.
来源期刊	TALANTA
ISSN	0039-9140
EISSN	1873-3573
出版年	2022
卷号	236
英文摘要	Glucagon-like peptide (GLP)-1 and the glucose-dependent insulinotropic peptide (GIP) are incretin hormones that regulate the nutrient-stimulated insulin secretion from pancreatic 13-cells. Their low plasma concentrations and rapid clearance pose certain methodological challenges for their detection and quantification. The currently available immunomediated techniques to monitor these hormones overestimate, to some extent, their actual concentration. Hence, the present study is aimed at developing a robust and reliable methodology for the identification and quantification of active and inactive forms of the incretins GLP-1 and GIP, in human plasma, by UHPLC-ESI-QqQ-MS/MS. A comparative study of different SPE cartridges was carried out, being identified OASIS HLB as the most efficient one, with recoveries up to 80%. The method provides adequate linearity, from 4.88 to 1250 nM, and low intervals of LOD and LOQ for each analyte (ranges from 0.01 to 3.42 nM and from 0.10 to 34.17 nM, respectively). The methodology described was validated upon a clinical trial with overweight subjects (n = 20) (ClinicalTrials.gov NCT 04016337), showing the capacity of the newly developed methodology to detect the augment of the plasma concentration of both GLP-1(7-36) and GLP-1(9-36) between 30 and 60 min after the consumption of a sucrose sweetened fruit-based beverage, while the plasma concentration of GIP remained in levels lower than the LOQ. The proposed methodology provides further insights into the mechanisms of action of bioactive compounds and food components in the frame of the glycemic control and would contribute to the assessment of the efficacy of antidiabetic drugs.
英文关键词	Incretins UHPLC/MS/MS validation Insulin resistance Citrus Maqui Sweeteners
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000701264100002
WOS关键词	GLUCAGON-LIKE PEPTIDE-1 ; COMMERCIALLY AVAILABLE ASSAYS ; TANDEM MASS-SPECTROMETRY ; POLYPEPTIDES GIP(1-42) ; QUANTIFICATION ; IMMUNOPRECIPITATION ; QUANTITATION ; SPECIFICITY ; SENSITIVITY ; GIP(3-42)
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/364776
作者单位	[Villano, Debora; Zafrilla, Pilar] Univ Catolica San Antonio Murcia UCAM, Fac Hlth Sci, Sch Pharm, Res Grp Nutr & Oxidat Stress, Campus Jeronimos, Murcia 30107, Spain; [Garcia-Viguera, Cristina; Dominguez-Perles, Raul] Univ Campus Espinardo, Phytochem & Hlth Foods Lab LabFAS, Dept Food Sci & Technol CEBAS CSIC, Edif 25, Murcia 30100, Spain
推荐引用方式 GB/T 7714	Villano, Debora,Zafrilla, Pilar,Garcia-Viguera, Cristina,et al. A UHPLC/MS/MS method for the analysis of active and inactive forms of GLP-1 and GIP incretins in human plasma[J],2022,236.
APA	Villano, Debora,Zafrilla, Pilar,Garcia-Viguera, Cristina,&Dominguez-Perles, Raul.(2022).A UHPLC/MS/MS method for the analysis of active and inactive forms of GLP-1 and GIP incretins in human plasma.TALANTA,236.
MLA	Villano, Debora,et al."A UHPLC/MS/MS method for the analysis of active and inactive forms of GLP-1 and GIP incretins in human plasma".TALANTA 236(2022).