干旱区生态环境知识资源中心(Arid): Squash root microbiome transplants and metagenomic inspection for in situ arid adaptations

Arid

DOI	10.1016/j.scitotenv.2021.150136
	Squash root microbiome transplants and metagenomic inspection for in situ arid adaptations
	Hernandez-Alvarez, Cristobal; Garcia-Oliva, Felipe; Cruz-Ortega, Rocio; Romero, Miguel F.; Barajas, Hugo R.; Pinero, Daniel; Alcaraz, Luis D.
通讯作者	Alcaraz, LD (corresponding author), Univ Nacl Autonoma Mexico, Circuit Exterior, Mexico City 04510, DF, Mexico.
来源期刊	SCIENCE OF THE TOTAL ENVIRONMENT
ISSN	0048-9697
EISSN	1879-1026
出版年	2022
卷号	805
英文摘要	Arid zones contain a diverse set of microbes capable of survival under dry conditions, some of which can form relationships with plants under drought stress conditions to improve plant health. We studied squash (Cucurbita pepo L.) root microbiome under historically arid and humid sites, both in situ and performing a common garden experiment. Plants were grown in soils from sites with different drought levels, using in situ collected soils as the microbial source. We described and analyzed bacterial diversity by 16S rRNA gene sequencing (N = 48) from the soil, rhizosphere, and endosphere. Proteobacteria were the most abundant phylum present in humid and arid samples, while Actinobacteriota abundance was higher in arid ones. The beta-diversity analyses showed split microbiomes between arid and humid microbiomes, and aridity and soil pH levels could explain it. These differences between humid and arid microbiomes were maintained in the common garden experiment, showing that it is possible to transplant in situ diversity to the greenhouse. We detected a total of 1009 bacterial genera; 199 exclusively associated with roots under arid conditions. By 16S and shotgun metagenomics, we identified dry-associated taxa such as Cellvibrio, Ensifer adhaerens, and Streptomyces flavovariabilis. With shotgun metagenomic sequencing of rhizospheres (N = 6), we identified 2969 protein families in the squash core metagenome and found an increased number of exclusively protein families from arid (924) than humid samples (158). We found arid conditions enriched genes involved in protein degradation and folding, oxidative stress, compatible solute synthesis, and ion pumps associated with osmotic regulation. Plant phenotyping allowed us to correlate bacterial communities with plant growth. Our study revealed that it is possible to evaluate microbiome diversity ex-situ and identify critical species and genes involved in plant-microbe interactions in historically arid locations. (c) 2021 Elsevier B.V. All rights reserved.
英文关键词	Arid ecosystems Rhizosphere Root endosphere Microbiome Metagenomics Squash (Cucurbita pepo)
类型	Article
语种	英语
开放获取类型	Green Submitted
收录类别	SCI-E
WOS记录号	WOS:000704385400010
WOS关键词	BACTERIAL COMMUNITY STRUCTURE ; SP-NOV. ; ENDOPHYTIC COLONIZATION ; READ ALIGNMENT ; LEAF-AREA ; GEN.-NOV. ; SOIL ; PROTEIN ; BIOSYNTHESIS ; PHOSPHATE
WOS类目	Environmental Sciences
WOS研究方向	Environmental Sciences & Ecology
来源机构	Universidad Nacional Autónoma de México
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/364620
作者单位	[Hernandez-Alvarez, Cristobal; Romero, Miguel F.; Barajas, Hugo R.; Alcaraz, Luis D.] Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Biol Celular, Lab Genom Ambiental, Mexico City, DF, Mexico; [Hernandez-Alvarez, Cristobal] Univ Nacl Autonoma Mexico, Posgrad Ciencias Biol, Mexico City, DF, Mexico; [Garcia-Oliva, Felipe] Univ Nacl Autonoma Mexico, Inst Invest Ecosistemas & Sustentabilidad, Mexico City, DF, Mexico; [Cruz-Ortega, Rocio] Univ Nacl Autonoma Mexico, Inst Ecol, Dept Ecol Func, Mexico City, DF, Mexico; [Pinero, Daniel] Univ Nacl Autonoma Mexico, Inst Ecol, Dept Ecol Evolut, Mexico City, DF, Mexico
推荐引用方式 GB/T 7714	Hernandez-Alvarez, Cristobal,Garcia-Oliva, Felipe,Cruz-Ortega, Rocio,et al. Squash root microbiome transplants and metagenomic inspection for in situ arid adaptations[J]. Universidad Nacional Autónoma de México,2022,805.
APA	Hernandez-Alvarez, Cristobal.,Garcia-Oliva, Felipe.,Cruz-Ortega, Rocio.,Romero, Miguel F..,Barajas, Hugo R..,...&Alcaraz, Luis D..(2022).Squash root microbiome transplants and metagenomic inspection for in situ arid adaptations.SCIENCE OF THE TOTAL ENVIRONMENT,805.
MLA	Hernandez-Alvarez, Cristobal,et al."Squash root microbiome transplants and metagenomic inspection for in situ arid adaptations".SCIENCE OF THE TOTAL ENVIRONMENT 805(2022).