干旱区生态环境知识资源中心(Arid): Exploring untapped potential of Streptomyces spp. in Gurbantunggut Desert by use of highly selective culture strategy

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DOI	10.1016/j.scitotenv.2021.148235
	Exploring untapped potential of Streptomyces spp. in Gurbantunggut Desert by use of highly selective culture strategy
	Li, Shuai; Dong, Lei; Lian, Wen-Hui; Lin, Zhi-Liang; Lu, Chun-Yan; Xu, Lu; Li, Li; Hozzein, Wael N.; Li, Wen-Jun
通讯作者	Dong, L ; Li, WJ (corresponding author), Sun Yat Sen Univ, Guangzhou 510275, Peoples R China.
来源期刊	SCIENCE OF THE TOTAL ENVIRONMENT
ISSN	0048-9697
EISSN	1879-1026
出版年	2021
卷号	790
英文摘要	Streptomycetes have been, for over 70 years, one of the most abundant sources for the discovery of new antibiotics and clinic drugs. However, in recent decades, it has been more and more difficult to obtain new phylotypes of the genus Streptomyces by using conventional samples and culture strategies. In this study, we combined culture-dependent and culture-independent approaches to better explore the Streptomyces communities in desert sandy soils. Moreover, two different culture strategies termed Conventional Culture Procedure (CCP) and Streptomycetes Culture Procedure (SCP) were employed to evaluate the isolation efficiency of Streptomyces spp. with different intensities of selectivity. The 16S rRNA gene amplicon analysis revealed a very low abundance (0.04-0.37%, average 0.22%) of Streptomyces in all the desert samples, conversely the percentage of Streptomyces spp. obtained by the culture-dependent method was very high (5.20-39.57%, average 27.76%), especially in the rhizospheric sand soils (38.40-39.57%, average 38.99%). Meanwhile, a total of 1589 pure cultures were isolated successfully, dominated by Streptomyces (29.52%), Microvirga (8.06%) and Bacillus (7.68%). In addition, 400 potential new species were obtained, 48 of which belonged to the genus Streptomyces. More importantly, our study demonstrated the SCP strategy which had highly selectivity could greatly expand the number and phylotypes of Streptomyces spp. by almost 4-fold than CCP strategy. These results provide insights on the diversity investigation of desert Streptomyces, and it could be reference for researchers to bring more novel actinobacteria strains from the environment into culture. (C) 2021 Elsevier B.V. All rights reserved.
英文关键词	Culture-dependent Culture-independent Streptomycetes culture procedure (SCP) Conventional culture procedure (CCP) Streptomyces spp. Isolation efficiency
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000685284800021
WOS关键词	BACTERIAL DIVERSITY ; PLANT-GROWTH ; RHIZOSPHERE ; IDENTIFICATION ; MEGACOMPLEX ; ENUMERATION ; TAXONOMY ; TOMATO
WOS类目	Environmental Sciences
WOS研究方向	Environmental Sciences & Ecology
来源机构	中国科学院新疆生态与地理研究所 ; King Saud University
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/364568
作者单位	[Li, Shuai; Dong, Lei; Lian, Wen-Hui; Lin, Zhi-Liang; Lu, Chun-Yan; Xu, Lu; Li, Wen-Jun] Sun Yat Sen Univ, Sch Life Sci, Guangdong Prov Key Lab Plant Resources, State Key Lab Biocontrol, Guangzhou 510275, Peoples R China; [Li, Shuai; Dong, Lei; Lian, Wen-Hui; Lin, Zhi-Liang; Lu, Chun-Yan; Xu, Lu; Li, Wen-Jun] Sun Yat Sen Univ, Sch Life Sci, Southern Marine Sci & Engn Guangdong Lab Zhuhai, Guangzhou 510275, Peoples R China; [Li, Li] Chinese Acad Sci, Xinjiang Inst Ecol & Geog, State Key Lab Desert & Oasis Ecol, Urumqi 830011, Peoples R China; [Hozzein, Wael N.] King Saud Univ, Coll Sci, Zool Dept, Riyadh 999088, Saudi Arabia; [Hozzein, Wael N.] Beni Suef Univ, Fac Sci, Bot & Microbiol Dept, Bani Suwayf, Egypt
推荐引用方式 GB/T 7714	Li, Shuai,Dong, Lei,Lian, Wen-Hui,et al. Exploring untapped potential of Streptomyces spp. in Gurbantunggut Desert by use of highly selective culture strategy[J]. 中国科学院新疆生态与地理研究所, King Saud University,2021,790.
APA	Li, Shuai.,Dong, Lei.,Lian, Wen-Hui.,Lin, Zhi-Liang.,Lu, Chun-Yan.,...&Li, Wen-Jun.(2021).Exploring untapped potential of Streptomyces spp. in Gurbantunggut Desert by use of highly selective culture strategy.SCIENCE OF THE TOTAL ENVIRONMENT,790.
MLA	Li, Shuai,et al."Exploring untapped potential of Streptomyces spp. in Gurbantunggut Desert by use of highly selective culture strategy".SCIENCE OF THE TOTAL ENVIRONMENT 790(2021).