干旱区生态环境知识资源中心(Arid): An automated streamlined method for the therapeutic drug monitoring of digoxin based on the online-solid-phase extraction liquid chromatography tandem high-resolution mass spectrometry

Arid

DOI	10.1002/rcm.9191
	An automated streamlined method for the therapeutic drug monitoring of digoxin based on the online-solid-phase extraction liquid chromatography tandem high-resolution mass spectrometry
	Liu, Congmin; Wang, Li; Pan, Lixuan; Hou, Wenqing; Hao, Zhihui; Qu, Lihua
通讯作者	Qu, LH (corresponding author), Qingdao Agr Univ, Coll Chem & Pharmaceut Sci, Qingdao 266109, Peoples R China. ; Hao, ZH (corresponding author), China Agr Univ, Coll Vet Med, Chinese Vet Med Innovat Ctr, Beijing 100193, Peoples R China.
来源期刊	RAPID COMMUNICATIONS IN MASS SPECTROMETRY
ISSN	0951-4198
EISSN	1097-0231
出版年	2021
卷号	35 期号:22
英文摘要	Rationale Digoxin is widely used in the clinical treatment of cardiovascular diseases. However, due to its extremely narrow therapeutic window, therapeutic drug monitoring (TDM) is vitally important. In consideration of the time-consuming and labor-intensive nature of the traditional techniques, an automated and efficient method was required for the clinical individualized TDM of digoxin. Methods An online solid-phase extraction liquid chromatography tandem high-resolution mass spectrometry (online-SPE-LC-HRMS) method was developed and applied for the determination of digoxin in plasma. The online SPE-LC steps included pretreatment and separation of plasma samples that were carried out using a Waters Oasis HLB cartridge and XBridge Shield RP18 column, respectively. A high-resolution Q Orbitrap mass spectrometer with targeted-selected ion monitoring in negative scan mode was applied to monitor formate-adduct ions [M + HCOO](-) m/z 825.42781 for digoxin. Results Linearity was shown over the range 0.1-10 ng mL(-1) for digoxin with correlation coefficients of R-2 > 0.999. The lower limit of quantitation (LLOQ) for digoxin was 0.1 ng mL(-1). Extraction recoveries ranged from 82.61% to 94.28% for digoxin. The intra- and inter-day precision values were < 5.53% with accuracy ranging from 84.97% to 96.75%. The total running time was 10 min for each sample. Conclusion The established method displayed satisfactory recoveries, accuracy, precision, and stability, and successfully applied on the TDM of digoxin. This automated streamlined method provides a powerful tool to guide the individualized administration of digoxin, which is significant for the practice of precision medicine.
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000706436700002
WOS关键词	LC-MS ASSAY ; SIMULTANEOUS QUANTIFICATION ; SPE-LC ; HUMAN PLASMA ; PERFORMANCE ; VALIDATION ; DIGITOXIN
WOS类目	Biochemical Research Methods ; Chemistry, Analytical ; Spectroscopy
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy
来源机构	中国农业大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/364455
作者单位	[Liu, Congmin; Pan, Lixuan; Hou, Wenqing; Qu, Lihua] Qingdao Agr Univ, Coll Chem & Pharmaceut Sci, Qingdao 266109, Peoples R China; [Wang, Li] Qingdao Univ, Dept Integrated Chinese & Western Med, Affiliated Yantai Yuhuangding Hosp, Med Coll, Yantai, Peoples R China; [Hao, Zhihui] China Agr Univ, Coll Vet Med, Chinese Vet Med Innovat Ctr, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Liu, Congmin,Wang, Li,Pan, Lixuan,et al. An automated streamlined method for the therapeutic drug monitoring of digoxin based on the online-solid-phase extraction liquid chromatography tandem high-resolution mass spectrometry[J]. 中国农业大学,2021,35(22).
APA	Liu, Congmin,Wang, Li,Pan, Lixuan,Hou, Wenqing,Hao, Zhihui,&Qu, Lihua.(2021).An automated streamlined method for the therapeutic drug monitoring of digoxin based on the online-solid-phase extraction liquid chromatography tandem high-resolution mass spectrometry.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,35(22).
MLA	Liu, Congmin,et al."An automated streamlined method for the therapeutic drug monitoring of digoxin based on the online-solid-phase extraction liquid chromatography tandem high-resolution mass spectrometry".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 35.22(2021).