干旱区生态环境知识资源中心(Arid): Chloroplastic SaNADP-ME4 of C-3-C-4 Woody Desert Species Salsola laricifolia Confers Drought and Salt Stress Resistance to Arabidopsis

Arid

DOI	10.3390/plants10091827
	Chloroplastic SaNADP-ME4 of C-3-C-4 Woody Desert Species Salsola laricifolia Confers Drought and Salt Stress Resistance to Arabidopsis
	Wen, Zhibin; Wang, Yulan; Xia, Chunlan; Zhang, Yuhui; Zhang, Hongxiang
通讯作者	Zhang, HX (corresponding author), Chinese Acad Sci, Xinjiang Inst Ecol & Geog, State Key Lab Desert & Oasis Ecol, Urumqi 830011, Peoples R China. ; Zhang, HX (corresponding author), Chinese Acad Sci, Specimen Museum, Xinjiang Inst Ecol & Geog, Urumqi 830011, Peoples R China. ; Zhang, HX (corresponding author), Univ Chinese Acad Sci, Beijing 100049, Peoples R China.
来源期刊	PLANTS-BASEL
EISSN	2223-7747
出版年	2021
卷号	10 期号:9
英文摘要	The NADP-malic enzyme (NADP-ME) catalyzes the reversible decarboxylation of L-malate to produce pyruvate, CO2, and NADPH in the presence of a bivalent cation. In addition, this enzyme plays crucial roles in plant developmental and environment responses, especially for the plastidic isoform. However, this isoform is less studied in C-3-C-4 intermediate species under drought and salt stresses than in C-3 and C-4 species. In the present study, we characterized SaNADP-ME4 from the intermediate woody desert species Salsola laricifolia. SaNADP-ME4 encoded a protein of 646 amino acids, which was found to be located in the chloroplasts based on confocal imaging. Quantitative real-time PCR analysis showed that SaNADP-ME4 was highly expressed in leaves, followed by stems and roots, and SaNADP-ME4 expression was improved and reached its maximum under the 200 mm mannitol and 100 mm NaCl treatments, respectively. Arabidopsis overexpressing SaNADP-ME4 showed increased root length and fresh weight under mannitol and salt stress conditions at the seedling stage. In the adult stage, SaNADP-ME4 could alleviate the decreased in chlorophyll contents and PSII photochemical efficiency, as well as improve the expression of superoxide dismutase, peroxidase, and pyrroline-5-carboxylate synthase genes to enhance reactive oxygen species scavenging capability and proline levels. Our results suggest that SaNADP-ME4 overexpression in Arabidopsis increases drought and salt stress resistance.
英文关键词	SaNADP-ME4 C-3-C-4 intermediate species abiotic stress Salsola laricifolia
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000701169300001
WOS关键词	NADP-MALIC ENZYME ; TIME QUANTITATIVE PCR ; GENE FAMILY ; C-4 ; EXPRESSION ; TOLERANCE ; PLANTS ; PROTEIN ; DEHYDROGENASE ; EVOLUTION
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
来源机构	中国科学院新疆生态与地理研究所
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/364364
作者单位	[Wen, Zhibin; Wang, Yulan; Xia, Chunlan; Zhang, Yuhui; Zhang, Hongxiang] Chinese Acad Sci, Xinjiang Inst Ecol & Geog, State Key Lab Desert & Oasis Ecol, Urumqi 830011, Peoples R China; [Wen, Zhibin; Zhang, Hongxiang] Chinese Acad Sci, Specimen Museum, Xinjiang Inst Ecol & Geog, Urumqi 830011, Peoples R China; [Wen, Zhibin; Wang, Yulan; Xia, Chunlan; Zhang, Yuhui; Zhang, Hongxiang] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wen, Zhibin,Wang, Yulan,Xia, Chunlan,et al. Chloroplastic SaNADP-ME4 of C-3-C-4 Woody Desert Species Salsola laricifolia Confers Drought and Salt Stress Resistance to Arabidopsis[J]. 中国科学院新疆生态与地理研究所,2021,10(9).
APA	Wen, Zhibin,Wang, Yulan,Xia, Chunlan,Zhang, Yuhui,&Zhang, Hongxiang.(2021).Chloroplastic SaNADP-ME4 of C-3-C-4 Woody Desert Species Salsola laricifolia Confers Drought and Salt Stress Resistance to Arabidopsis.PLANTS-BASEL,10(9).
MLA	Wen, Zhibin,et al."Chloroplastic SaNADP-ME4 of C-3-C-4 Woody Desert Species Salsola laricifolia Confers Drought and Salt Stress Resistance to Arabidopsis".PLANTS-BASEL 10.9(2021).