干旱区生态环境知识资源中心(Arid): Chemical Profile, Antioxidant, Antimicrobial, and Anticancer Activities of the Water-Ethanol Extract of Pulicaria undulata Growing in the Oasis of Central Saudi Arabian Desert

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DOI	10.3390/plants10091811
	Chemical Profile, Antioxidant, Antimicrobial, and Anticancer Activities of the Water-Ethanol Extract of Pulicaria undulata Growing in the Oasis of Central Saudi Arabian Desert
	Mohammed, Hamdoon A.; Al-Omar, Mohsen S.; Khan, Riaz A.; Mohammed, Salman A. A.; Qureshi, Kamal A.; Abbas, Manal M.; Al Rugaie, Osamah; Abd-Elmoniem, Essam; Ahmad, Adel M.; Kandil, Yasser, I
通讯作者	Mohammed, HA (corresponding author), Qassim Univ, Coll Pharm, Dept Med Chem & Pharmacognosy, Buraydah 51452, Saudi Arabia. ; Mohammed, HA (corresponding author), Al Azhar Univ, Fac Pharm, Dept Pharmacognosy, Cairo 11371, Egypt.
来源期刊	PLANTS-BASEL
EISSN	2223-7747
出版年	2021
卷号	10 期号:9
英文摘要	Pulicaria undulata (L.) C. A. Mey has multiple uses as part of the traditional medicament, and several biological activities of the plant have been corroborated in the scientific literature. The current work evaluates the phytochemical constituents and biological properties of the water-ethanol extract of the P. undulata growing in Qassim, the central arid regions of the Kingdom of Saudi Arabia. Qualitative UPLC-ESIQ-TOF analysis identified 27 compounds belonging to the phenolics, flavonoids, triterpenes, coumarins, and of fatty acids chemical classes. The quantitative analysis exhibited 33.3 mg/g GAE (Gallic Acid Equivalents), and 10.8 mg/g QE (Quercetin Equivalents) of the phenolics and flavonoids in the plant's concentrated (to dryness) water-ethanol extract. The trace elements analysis of the plant's dry powder established the presence of copper (20.13 mu g/kg), and zinc (68.2 mu g/kg) in the higher levels of occurrences. In terms of the antioxidant potential of the plant's extract, the ferric-reducing, and free-radicals scavenging activities were recorded at 47.11 mg/g, and 19.13 mg/g equivalents of the concentrated to dryness water-ethanol extract of the plant. The water-ethanol extract of P. undulata also exhibited antimicrobial activity against the tested Gram-positive bacteria, while no activity was observed against the tested Gram-negative bacteria, or the fungi. The MIC (minimum inhibitory concentration) values were in the range of 49 to 1563 mu g/mL, whereas the MBC (minimum bactericidal concentration) values ranged from 49 to 3125 mu g/mL, against the tested Gram-positive bacteria. The P. undulata water-ethanol extract also exhibited potent cytotoxic effects with the IC50 value at 519.2 mu g/mL against the MCF-7 breast cancer cell-lines, followed by the anticancer activity of erythroleukemic cell-lines, K562 at 1212 mu g/mL, and pancreatic cell-lines, PANC-1, at 1535 mu g/mL, as compared to the normal fibroblast cells (4048 mu g/mL). The Annexin-V assay demonstrated that, as the P. undulata extract's dose increased from IC50 to twice of the IC50, the percentage of the necrosis was found to be increased in the late apoptosis stage of the cancer cells. These data confirmed the P. undulata extract's ability to inhibit several human cancer cell lines' growth in comparison to other local halophytes. The antimicrobial activity of the plant was also confirmed.
英文关键词	Pulicaria undulata phenolics flavonoids anticancer antioxidant antimicrobial Annexin V MCF-7 K562 PANC-1
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000701116900001
WOS关键词	AGRICULTURAL SOILS ; ESSENTIAL OIL ; HEAVY-METALS ; BIOLOGICAL-ACTIVITIES ; IN-VITRO ; HALOPHYTES ; STRESS ; AREA ; L. ; ACCUMULATION
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/364361
作者单位	[Mohammed, Hamdoon A.; Al-Omar, Mohsen S.; Khan, Riaz A.] Qassim Univ, Coll Pharm, Dept Med Chem & Pharmacognosy, Buraydah 51452, Saudi Arabia; [Mohammed, Hamdoon A.] Al Azhar Univ, Fac Pharm, Dept Pharmacognosy, Cairo 11371, Egypt; [Al-Omar, Mohsen S.] JUST, Dept Med Chem & Pharmacognosy, Fac Pharm, Irbid 22110, Jordan; [Mohammed, Salman A. A.] Qassim Univ, Coll Pharm, Dept Pharmacol & Toxicol, Buraydah 51452, Saudi Arabia; [Qureshi, Kamal A.] Qassim Univ, Unaizah Coll Pharm, Dept Pharmaceut, Unaizah 51911, Saudi Arabia; [Abbas, Manal M.] Al Ahliyya Amman Univ, Fac Allied Med Sci, Dept Med Lab Sci, Amman 19328, Jordan; [Al Rugaie, Osamah] Qassim Univ, Coll Med & Med Sci, Dept Basic Med Sci, Unaizah 51911, Saudi Arabia; [Abd-Elmoniem, Essam] Qassim Univ, Coll Agr & Vet Med, Dept Plant Prod & Protect, Qasim 51452, Saudi Arabia; [Ahmad, Adel M.] South Valley Univ, Fac Pharm, Dept Pharmaceut Analyt Chem, Qena 83523, Egypt; [Kandil, Yasser, I] Al Azhar Univ, Fac Pharm, Biochem & Mol Biol Dept, Cairo 11231, Egypt
推荐引用方式 GB/T 7714	Mohammed, Hamdoon A.,Al-Omar, Mohsen S.,Khan, Riaz A.,et al. Chemical Profile, Antioxidant, Antimicrobial, and Anticancer Activities of the Water-Ethanol Extract of Pulicaria undulata Growing in the Oasis of Central Saudi Arabian Desert[J],2021,10(9).
APA	Mohammed, Hamdoon A..,Al-Omar, Mohsen S..,Khan, Riaz A..,Mohammed, Salman A. A..,Qureshi, Kamal A..,...&Kandil, Yasser, I.(2021).Chemical Profile, Antioxidant, Antimicrobial, and Anticancer Activities of the Water-Ethanol Extract of Pulicaria undulata Growing in the Oasis of Central Saudi Arabian Desert.PLANTS-BASEL,10(9).
MLA	Mohammed, Hamdoon A.,et al."Chemical Profile, Antioxidant, Antimicrobial, and Anticancer Activities of the Water-Ethanol Extract of Pulicaria undulata Growing in the Oasis of Central Saudi Arabian Desert".PLANTS-BASEL 10.9(2021).