干旱区生态环境知识资源中心(Arid): Pretreatment of seeds with hydrogen peroxide improves deep-sowing tolerance of wheat seedlings

Arid

DOI	10.1016/j.plaphy.2021.08.016
	Pretreatment of seeds with hydrogen peroxide improves deep-sowing tolerance of wheat seedlings
	Cetinel, Askim Hediye Sekmen; Yalcinkaya, Tolga; Akyol, Turgut Yigit; Gokce, Azime; Turkan, Ismail
通讯作者	Turkan, I (corresponding author), Ege Univ, Fac Sci, Dept Biol, TR-35100 Izmir, Turkey.
来源期刊	PLANT PHYSIOLOGY AND BIOCHEMISTRY
ISSN	0981-9428
EISSN	1873-2690
出版年	2021
卷号	167 页码:321-336
英文摘要	Drought is a prevalent natural factor limiting crop production in arid regions across the world. To overcome this limitation, seeds are sown much deeper to boost germination by soil moisture produced by underground water. Seed pretreatment can effectively induce deep-sowing tolerance in plants. In the present study, we evaluated whether H2O2 pretreatment of seeds can initiate metabolic changes and lead to improved deep-sowing tolerance in wheat. Pretreatment with 0.05 mu M H2O2 promoted first internode elongation by 13% in the deep-sowing tolerant wheat cultivar Tir and by 32% in the sensitive cultivar Kirac-66 under deep-sowing conditions, whereas internode elongation was inhibited by diphenyleneiodonium chloride. In contrast to Tir seedlings, H2O2 levels in the first internode of Kirac-66 seedlings increased under deep-sowing condition in the H2O2-treated group compared to controls. Moreover, these seedlings had significantly lower catalase (CAT), peroxidase (POX), and ascorbate peroxidase (APX) activities but higher NADPH oxidase (NOX) and superoxide dismutase (SOD) activities under the same conditions, which consequently induced greater H2O2 accumulation. Contrary to Tir, both total glutathione and glutathione S-transferase (GST) activity decreased in Kirac-66 after deep-sowing at 10 cm. However, H2O2 treatment increased the total glutathione amounts and the activities of glutathione-related enzymes (except GST and GPX) in the first internode of Kirac-66. Taken together, these data support that H2O2 acts as a signaling molecule in the activation of antioxidant enzymes (specifically NOX, SOD, and CAT), regulation of both glutathione-related enzymes and total glutathione content, and upregulation of the cell wall-loosening protein gene TaEXPB23.
英文关键词	Antioxidant enzymes Deep-sowing First internode elongation Glutathione Hydrogen peroxide Wheat
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000703775900028
WOS关键词	ADVENTITIOUS ROOT-FORMATION ; ANTIOXIDANT ENZYMES ; GENE-EXPRESSION ; 1ST INTERNODES ; NADPH OXIDASE ; IN-VITRO ; GLUTATHIONE ; GROWTH ; ELONGATION ; PROTEINS
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/364344
作者单位	[Cetinel, Askim Hediye Sekmen; Yalcinkaya, Tolga; Gokce, Azime; Turkan, Ismail] Ege Univ, Fac Sci, Dept Biol, TR-35100 Izmir, Turkey; [Akyol, Turgut Yigit] Aarhus Univ, Dept Mol Biol & Genet, DK-8000 Aarhus, Denmark
推荐引用方式 GB/T 7714	Cetinel, Askim Hediye Sekmen,Yalcinkaya, Tolga,Akyol, Turgut Yigit,et al. Pretreatment of seeds with hydrogen peroxide improves deep-sowing tolerance of wheat seedlings[J],2021,167:321-336.
APA	Cetinel, Askim Hediye Sekmen,Yalcinkaya, Tolga,Akyol, Turgut Yigit,Gokce, Azime,&Turkan, Ismail.(2021).Pretreatment of seeds with hydrogen peroxide improves deep-sowing tolerance of wheat seedlings.PLANT PHYSIOLOGY AND BIOCHEMISTRY,167,321-336.
MLA	Cetinel, Askim Hediye Sekmen,et al."Pretreatment of seeds with hydrogen peroxide improves deep-sowing tolerance of wheat seedlings".PLANT PHYSIOLOGY AND BIOCHEMISTRY 167(2021):321-336.