干旱区生态环境知识资源中心(Arid): Integrated transcriptomics and metabolomics analysis to characterize alkali stress responses in canola (Brassica napus L.)

Arid

DOI	10.1016/j.plaphy.2021.06.021
	Integrated transcriptomics and metabolomics analysis to characterize alkali stress responses in canola (Brassica napus L.)
	Wang, Weichao; Pang, Jiayin; Zhang, Fenghua; Sun, Lupeng; Yang, Lei; Zhao, Yaguang; Yang, Yang; Wang, Yajuan; Siddique, Kadambot H. M.
通讯作者	Zhang, FH (corresponding author), Shihezi Univ, Key Lab Oasis Ecoagr, Xinjiang Prod & Construct Crops, Shihezi 832003, Xinjiang, Peoples R China.
来源期刊	PLANT PHYSIOLOGY AND BIOCHEMISTRY
ISSN	0981-9428
EISSN	1873-2690
出版年	2021
卷号	166 页码:605-620
英文摘要	Background: Soil salinization is a major constraint limiting agricultural development and affecting crop growth and productivity, especially in arid and semi-arid regions. Understanding the molecular mechanism of the adaptability of canola to salt stress is very important to improve the salt tolerance of canola and promote its cultivation in saline alkali soil. Results: To identify the metabolomic and transcriptomic mechanisms of canola under alkaline salt stress, we collected roots of control (no salt treatment) and 72 h Na2CO3-stressed canola seedlings (hydroponics) for metabolic profiling of metabolites, supplemented with RNA-Seq analysis and real-time quantitative PCR validation. Metabolomic analysis showed that the metabolites of amino acids and fatty acids were higher accumulated under alkaline salt stress, including L-proline, L-glutamate, L-histidine, L-phenylalanine, L-citrulline, Ltyrosine, L-saccharopine, L-tryptophan, linoleic acid, dihomo gamma linolenic acid, alpha linolenic acid, Eric acid, oleic acid and neuronic acid, while the metabolism of carbohydrate (sucrase, alpha, alpha trehalose), polyol (ribitol), UDP-D-galactose, D-mannose, D-fructose and D-glucose 6-phosphate decreased. Transcriptomic and metabolomic pathway analysis indicated that carbohydrate metabolism may not play an important role in the resistance of canola to alkaline salt stress. Organic acid metabolism (fatty acid accumulation) and amino acid metabolism are important metabolic pathways in the root of canola under alkaline salt stress. Conclusions: These results suggest that the genes and metabolites involved in fatty acid metabolism and amino acids metabolism in roots of canola may regulate salt tolerance of canola seedlings under alkaline salt stress, which improves our understanding of the molecular mechanisms of salt tolerance in canola.
英文关键词	Alkaline salt stress Transcriptome Metabolomic Metabolic pathway Amino acid
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000692319900003
WOS关键词	SALT-STRESS ; SALICYLIC-ACID ; ION BALANCE ; PHYSIOLOGICAL-RESPONSES ; PROLINE ACCUMULATION ; SOLUTE ACCUMULATION ; KOCHIA-SIEVERSIANA ; SALINITY TOLERANCE ; OSMOTIC ADJUSTMENT ; MALUS-HALLIANA
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
来源机构	University of Western Australia
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/364343
作者单位	[Wang, Weichao; Zhang, Fenghua; Sun, Lupeng; Yang, Lei; Zhao, Yaguang; Yang, Yang; Wang, Yajuan] Shihezi Univ, Key Lab Oasis Ecoagr, Xinjiang Prod & Construct Crops, Shihezi 832003, Xinjiang, Peoples R China; [Wang, Weichao; Pang, Jiayin; Siddique, Kadambot H. M.] Univ Western Australia, UWA Inst Agr, Perth, WA 6001, Australia; [Wang, Weichao; Pang, Jiayin; Siddique, Kadambot H. M.] Univ Western Australia, Sch Agr & Environm, Perth, WA 6001, Australia
推荐引用方式 GB/T 7714	Wang, Weichao,Pang, Jiayin,Zhang, Fenghua,et al. Integrated transcriptomics and metabolomics analysis to characterize alkali stress responses in canola (Brassica napus L.)[J]. University of Western Australia,2021,166:605-620.
APA	Wang, Weichao.,Pang, Jiayin.,Zhang, Fenghua.,Sun, Lupeng.,Yang, Lei.,...&Siddique, Kadambot H. M..(2021).Integrated transcriptomics and metabolomics analysis to characterize alkali stress responses in canola (Brassica napus L.).PLANT PHYSIOLOGY AND BIOCHEMISTRY,166,605-620.
MLA	Wang, Weichao,et al."Integrated transcriptomics and metabolomics analysis to characterize alkali stress responses in canola (Brassica napus L.)".PLANT PHYSIOLOGY AND BIOCHEMISTRY 166(2021):605-620.