干旱区生态环境知识资源中心(Arid): CLINICO-HEMATOLOGICAL, PATHO-ANATOMICAL AND MOLECULAR BASED INVESTIGATION OF BLACKLEG DISEASE IN CHOLISTANI CATTLE

Arid

DOI	10.21162/PAKJAS/21.1240
	CLINICO-HEMATOLOGICAL, PATHO-ANATOMICAL AND MOLECULAR BASED INVESTIGATION OF BLACKLEG DISEASE IN CHOLISTANI CATTLE
	Hussain, Riaz; Ehtisham-ul-Haque, Syed; Khan, Iahtasham; Jabeen, Ghazala; Siddique, Abu Baker; Ghaffar, Abdul; Abbas, Rao Zahid; Qudratullah; Ishaq, Hafiz Muhammad; Javed, Muhammad Tariq; Tariq, Narmeen; Ghori, Muhammad Taslim; Qayyum, Abdul; Abbas, Asghar; Jalees, Muhammad Moazzam; Wareth, Gamal
通讯作者	Hussain, R (corresponding author), Islamia Univ Bahawalpur, Fac Vet & Anim Sci, Dept Pathol, Bahawalpur 63100, Pakistan.
来源期刊	PAKISTAN JOURNAL OF AGRICULTURAL SCIENCES
ISSN	0552-9034
EISSN	2076-0906
出版年	2021
卷号	58 期号:3 页码:1017-1025
英文摘要	Clostridium chauvoei, causing myonecrosis in livestock animals, lives in the feces, surface water and soil. The blackleg due to C. chauvoei is very common in dairy animals. Still, no report is available about the pathophysiology of disease in Cholistani cattle kept under tropical and desert conditions of Cholistan, Pakistan. Therefore, in this study, we report for the first time the pathophysiology of a visceral form of blackleg infection in indigenous cholistani cattle breed reared in desert conditions of Cholistan. Clinically morbid animals exhibited different signs of infection including fever, crepitation sounds, gaseous swelling and edematous lesions. Blood was collected for hematological and serum biochemical investigation. Hematological examination indicated a significant increased erythrocyte sedimentation rate, lower red blood cells, hematocrit, and total white blood cell count. Results on serum biochemistry showed significantly (p<0.02) increased creatine kinase levels, creatinine, urea, alanine aminotransferase, aspartate aminotransferase, alkaline phosphates, creatinine phosphokinase and lactate dehydrogenase in infected cattle. At necropsy level, light pink color fluid under swelling areas of skin, gas bubbles, dark to black in color of affected muscles and crepitation sounds at palpation were observed. Necropsy showed marked myocarditis, petechial hemorrhages, consolidation and severe pulmonary edema. Spleen showed petechial hemorrhages and congestion. Histological analysis of muscular tissues indicated severe inflammatory reaction comprising of cellular infiltration, marked edema, necrosis and disruption of myofibrils. PCR confirmed the presence of C. chauvoei in muscles, heart and exudates of the lungs. This is the first report on molecular detection of Clostridium chauvoei from a visceral form of Blackleg disease in cholistani breed of cattle naturally infected in the Cholistan desert of Punjab, Pakistan.
英文关键词	Blackleg cholistani cattle blood biochemistry pathology PCR
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000684227300002
WOS关键词	CLOSTRIDIUM-CHAUVOEI ; FASCIOLA-HEPATICA ; PREVALENCE ; INFECTION ; PUNJAB ; RUMINANTS ; OUTBREAK ; NEURAMINIDASE ; MYOCARDITIS ; DIAGNOSIS
WOS类目	Agriculture, Multidisciplinary
WOS研究方向	Agriculture
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/364277
作者单位	[Hussain, Riaz] Islamia Univ Bahawalpur, Fac Vet & Anim Sci, Dept Pathol, Bahawalpur 63100, Pakistan; [Ehtisham-ul-Haque, Syed] Univ Vet & Anim Sci, Dept Pathobiol, Subcampus, Lahore, Jhang, Pakistan; [Khan, Iahtasham] Univ Vet & Anim Sci, Sect Epidemiol & Publ Hlth, Subcampus Jhang, Lahore, Pakistan; [Jabeen, Ghazala] Lahore Coll Women Univ, Dept Zool, Lahore, Pakistan; [Siddique, Abu Baker] Govt Coll Univ, Fac Sci & Technol, Dept Microbiol, Faisalabad 38040, Pakistan; [Ghaffar, Abdul] Islamia Univ Bahawalpur, Dept Life Sci Zool, Bahawalpur, Pakistan; [Abbas, Rao Zahid] Univ Agr Faisalabad, Fac Vet Sci, Dept Parasitol, Faisalabad 38040, Pakistan; [Qudratullah] Cholistan Univ Vet & Anim Sci, Dept Surg, Bahawalpur 63100, Pakistan; [Ishaq, Hafiz Muhammad; Abbas, Asghar] Muhammad Nawaz Shareef Univ Agr, Fac Vet & Anim Sci, Multan, Pakistan; [Javed, Muhammad Tariq] Univ Agr Faisalabad, Fac Vet Sci, Dept Pathol, Faisalabad 38040, Pakistan; [Tariq, Narmeen] Univ Vet & Anim Sci Lahore, Fac Vet Sci, Inst Microbio...
推荐引用方式 GB/T 7714	Hussain, Riaz,Ehtisham-ul-Haque, Syed,Khan, Iahtasham,et al. CLINICO-HEMATOLOGICAL, PATHO-ANATOMICAL AND MOLECULAR BASED INVESTIGATION OF BLACKLEG DISEASE IN CHOLISTANI CATTLE[J],2021,58(3):1017-1025.
APA	Hussain, Riaz.,Ehtisham-ul-Haque, Syed.,Khan, Iahtasham.,Jabeen, Ghazala.,Siddique, Abu Baker.,...&Wareth, Gamal.(2021).CLINICO-HEMATOLOGICAL, PATHO-ANATOMICAL AND MOLECULAR BASED INVESTIGATION OF BLACKLEG DISEASE IN CHOLISTANI CATTLE.PAKISTAN JOURNAL OF AGRICULTURAL SCIENCES,58(3),1017-1025.
MLA	Hussain, Riaz,et al."CLINICO-HEMATOLOGICAL, PATHO-ANATOMICAL AND MOLECULAR BASED INVESTIGATION OF BLACKLEG DISEASE IN CHOLISTANI CATTLE".PAKISTAN JOURNAL OF AGRICULTURAL SCIENCES 58.3(2021):1017-1025.