干旱区生态环境知识资源中心(Arid): Cryo-EM photosystem I structure reveals adaptation mechanisms to extreme high light in Chlorella ohadii

Arid

DOI	10.1038/s41477-021-00983-1
	Cryo-EM photosystem I structure reveals adaptation mechanisms to extreme high light in Chlorella ohadii
	Caspy, Ido; Neumann, Ehud; Fadeeva, Maria; Liveanu, Varda; Savitsky, Anton; Frank, Anna; Kalisman, Yael Levi; Shkolnisky, Yoel; Murik, Omer; Treves, Haim; Hartmann, Volker; Nowaczyk, Marc M.; Schuhmann, Wolfgang; Roegner, Matthias; Willner, Itamar; Kaplan, Aaron; Schuster, Gadi; Nelson, Nathan; Lubitz, Wolfgang; Nechushtai, Rachel
通讯作者	Nelson, N (corresponding author), Tel Aviv Univ, George S Wise Fac Life Sci, Dept Biochem, Tel Aviv, Israel. ; Nechushtai, R (corresponding author), Hebrew Univ Jerusalem, Fac Sci & Math, Inst Life Sci, Jerusalem, Israel. ; Lubitz, W (corresponding author), Max Planck Inst Chem Energy Convers, Mulheim, Germany.
来源期刊	NATURE PLANTS
ISSN	2055-026X
EISSN	2055-0278
出版年	2021
卷号	7 期号:9 页码:1314-+
英文摘要	Photosynthesis in deserts is challenging since it requires fast adaptation to rapid night-to-day changes, that is, from dawn's low light (LL) to extreme high light (HL) intensities during the daytime. To understand these adaptation mechanisms, we purified photosystem I (PSI) from Chlorella ohadii, a green alga that was isolated from a desert soil crust, and identified the essential functional and structural changes that enable the photosystem to perform photosynthesis under extreme high light conditions. The cryo-electron microscopy structures of PSI from cells grown under low light (PSILL) and high light (PSIHL), obtained at 2.70 and 2.71 angstrom, respectively, show that part of light-harvesting antenna complex I (LHCI) and the core complex subunit (PsaO) are eliminated from PSIHL to minimize the photodamage. An additional change is in the pigment composition and their number in LHCIHL; about 50% of chlorophyll b is replaced by chlorophyll a. This leads to higher electron transfer rates in PSIHL and might enable C. ohadii PSI to act as a natural photosynthesiser in photobiocatalytic systems. PSIHL or PSILL were attached to an electrode and their induced photocurrent was determined. To obtain photocurrents comparable with PSIHL, 25 times the amount of PSILL was required, demonstrating the high efficiency of PSIHL. Hence, we suggest that C. ohadii PSIHL is an ideal candidate for the design of desert artificial photobiocatalytic systems. Cryo-EM photosystem I structure reveals adaptation mechanisms to extreme high light in Chlorella ohadii.
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000691182100001
WOS关键词	ELECTRON-TRANSFER ; SINGLET OXYGEN ; STRESS ; PHOTODAMAGE ; PHOTOINHIBITION ; PHOTOPROTECTION ; SUPERCOMPLEX ; COMPLEX ; PHOTOSYNTHESIS ; EVOLUTION
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
来源机构	Hebrew University of Jerusalem
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/364233
作者单位	[Caspy, Ido; Fadeeva, Maria; Nelson, Nathan] Tel Aviv Univ, George S Wise Fac Life Sci, Dept Biochem, Tel Aviv, Israel; [Neumann, Ehud; Kalisman, Yael Levi; Murik, Omer; Treves, Haim; Willner, Itamar; Kaplan, Aaron; Nechushtai, Rachel] Hebrew Univ Jerusalem, Fac Sci & Math, Inst Life Sci, Jerusalem, Israel; [Liveanu, Varda; Schuster, Gadi] Technion Israel Inst Technol, Fac Biol, Haifa, Israel; [Savitsky, Anton] Tech Univ Dortmund, Fac Phys, Dortmund, Germany; [Savitsky, Anton; Lubitz, Wolfgang] Max Planck Inst Chem Energy Convers, Mulheim, Germany; [Frank, Anna; Hartmann, Volker; Nowaczyk, Marc M.; Roegner, Matthias] Ruhr Univ Bochum, Fac Biol & Biotechnol, Plant Biochem, Bochum, Germany; [Kalisman, Yael Levi] Hebrew Univ Jerusalem, Ctr Nanosci & Nanotechnol, Jerusalem, Israel; [Shkolnisky, Yoel] Tel Aviv Univ, Fac Exact Sci, Sch Math Sci, Tel Aviv, Israel; [Schuhmann, Wolfgang] Ruhr Univ Bochum, Fac Chem & Biochem, Analyt Chem Ctr Electrochem Sci CES, Bochum, Germany
推荐引用方式 GB/T 7714	Caspy, Ido,Neumann, Ehud,Fadeeva, Maria,et al. Cryo-EM photosystem I structure reveals adaptation mechanisms to extreme high light in Chlorella ohadii[J]. Hebrew University of Jerusalem,2021,7(9):1314-+.
APA	Caspy, Ido.,Neumann, Ehud.,Fadeeva, Maria.,Liveanu, Varda.,Savitsky, Anton.,...&Nechushtai, Rachel.(2021).Cryo-EM photosystem I structure reveals adaptation mechanisms to extreme high light in Chlorella ohadii.NATURE PLANTS,7(9),1314-+.
MLA	Caspy, Ido,et al."Cryo-EM photosystem I structure reveals adaptation mechanisms to extreme high light in Chlorella ohadii".NATURE PLANTS 7.9(2021):1314-+.