干旱区生态环境知识资源中心(Arid): Screening and Characterization of Two Extracellular Polysaccharide-Producing Bacteria from the Biocrust of the Mu Us Desert

Arid

DOI	10.3390/molecules26185521
	Screening and Characterization of Two Extracellular Polysaccharide-Producing Bacteria from the Biocrust of the Mu Us Desert
	Xue, Zhanfang; Zhao, Shuting; Bold, Nomin; Zhang, Jianguo; Hu, Zhimin; Hu, Xiaofeng; Gao, Ying; Chen, Shaolin; Wei, Yahong
通讯作者	Chen, SL ; Wei, YH (corresponding author), Northwest A&F Univ, Coll Life Sci, Biomass Energy Ctr Arid & Semiarid Lands, Shaanxi Key Lab Agr & Environm Microbiol, Xianyang 712100, Shaanxi, Peoples R China.
来源期刊	MOLECULES
EISSN	1420-3049
出版年	2021
卷号	26 期号:18
英文摘要	The extracellular polysaccharide (EPS) matrix embedding microbial cells and soil particles plays an important role in the development of biological soil crusts (BSCs), which is widely recognized as beneficial to soil fertility in dryland worldwide. This study examined the EPS-producing bacterial strains YL24-1 and YL24-3 isolated from sandy soil in the Mu Us Desert in Yulin, Shaanxi province, China. The strains YL24-1 and YL24-3 were able to efficiently produce EPS; the levels of EPS were determined to be 257.22 mu g/mL and 83.41 mu g/mL in cultures grown for 72 h and were identified as Sinorhizobium meliloti and Pedobacter sp., respectively. When the strain YL24-3 was compared to Pedobacter yulinensis YL28-9(T) using 16S rRNA gene sequencing, the resemblance was 98.6% and the strain was classified as Pedobacter sp. using physiological and biochemical analysis. Furthermore, strain YL24-3 was also identified as a subspecies of Pedobacter yulinensis YL28-9(T) on the basis of DNA-DNA hybridization and polar lipid analysis compared with YL28-9(T). On the basis of the EPS-related genes of relevant strains in the GenBank, several EPS-related genes were cloned and sequenced in the strain YL24-1, including those potentially involved in EPS synthesis, assembly, transport, and secretion. Given the differences of the strains in EPS production, it is possible that the differences in gene sequences result in variations in the enzyme/protein activities for EPS biosynthesis, assembly, transport, and secretion. The results provide preliminary evidence of various contributions of bacterial strains to the formation of EPS matrix in the Mu Us Desert.
英文关键词	extracellular polysaccharide Mu Us Desert biocrust bacterial polyphase classification
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000701788300001
WOS关键词	SP-NOV. ; EXOPOLYSACCHARIDE PRODUCTION ; EMENDED DESCRIPTION ; PEDOBACTER ; GENUS ; EXTRACTION ; SOIL ; CLASSIFICATION ; PURIFICATION ; PATHWAYS
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
来源机构	西北农林科技大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/364195
作者单位	[Xue, Zhanfang; Zhao, Shuting; Bold, Nomin; Hu, Zhimin; Gao, Ying; Chen, Shaolin; Wei, Yahong] Northwest A&F Univ, Coll Life Sci, Biomass Energy Ctr Arid & Semiarid Lands, Shaanxi Key Lab Agr & Environm Microbiol, Xianyang 712100, Shaanxi, Peoples R China; [Zhang, Jianguo] Northwest A&F Univ, Coll Nat Resources & Environm, Key Lab Plant Nutr & Agrienvironm Northwest China, Minist Agr, Xianyang 712100, Shaanxi, Peoples R China; [Hu, Xiaofeng] Shanghai Personal Biotechnol Co Ltd, Dept Next Generat Sequencing, Shanghai 200030, Peoples R China
推荐引用方式 GB/T 7714	Xue, Zhanfang,Zhao, Shuting,Bold, Nomin,et al. Screening and Characterization of Two Extracellular Polysaccharide-Producing Bacteria from the Biocrust of the Mu Us Desert[J]. 西北农林科技大学,2021,26(18).
APA	Xue, Zhanfang.,Zhao, Shuting.,Bold, Nomin.,Zhang, Jianguo.,Hu, Zhimin.,...&Wei, Yahong.(2021).Screening and Characterization of Two Extracellular Polysaccharide-Producing Bacteria from the Biocrust of the Mu Us Desert.MOLECULES,26(18).
MLA	Xue, Zhanfang,et al."Screening and Characterization of Two Extracellular Polysaccharide-Producing Bacteria from the Biocrust of the Mu Us Desert".MOLECULES 26.18(2021).