干旱区生态环境知识资源中心(Arid): Lab-In-Syringe with Bead Injection Coupled Online to High-Performance Liquid Chromatography as Versatile Tool for Determination of Nonsteroidal Anti-Inflammatory Drugs in Surface Waters

Arid

DOI	10.3390/molecules26175358
	Lab-In-Syringe with Bead Injection Coupled Online to High-Performance Liquid Chromatography as Versatile Tool for Determination of Nonsteroidal Anti-Inflammatory Drugs in Surface Waters
	Gemuh, Celestine Vubangsi; Horstkotte, Burkhard; Solich, Petr
通讯作者	Horstkotte, B (corresponding author), Charles Univ Prague, Fac Pharm Hradec Kralove, Dept Analyt Chem, Akad Heyrovskeho 1203, Hradec Kralove 50005, Czech Republic.
来源期刊	MOLECULES
EISSN	1420-3049
出版年	2021
卷号	26 期号:17
英文摘要	We report on the hyphenation of the modern flow techniques Lab-In-Syringe and Lab-On-Valve for automated sample preparation coupled online with high-performance liquid chromatography. Adopting the bead injection concept on the Lab-On-Valve platform, the on-demand, renewable, solid-phase extraction of five nonsteroidal anti-inflammatory drugs, namely ketoprofen, naproxen, flurbiprofen, diclofenac, and ibuprofen, was carried out as a proof-of-concept. In-syringe mixing of the sample with buffer and standards allowed straightforward pre-load sample modification for the preconcentration of large sample volumes. Packing of ca. 4.4 mg microSPE columns from Oasis HLB(R) sorbent slurry was performed for each sample analysis using a simple microcolumn adapted to the Lab-On-Valve manifold to achieve low backpressure during loading. Eluted analytes were injected into online coupled HPLC with subsequent separation on a Symmetry C18 column in isocratic mode. The optimized method was highly reproducible, with RSD values of 3.2% to 7.6% on 20 mu g L-1 level. Linearity was confirmed up to 200 mu g L-1 and LOD values were between 0.06 and 1.98 mu g L-1. Recovery factors between 91 and 109% were obtained in the analysis of spiked surface water samples.
英文关键词	Lab-In-Syringe Lab-On-Valve bead injection nonsteroidal anti-inflammatory drugs water analysis online coupling high-performance liquid chromatography
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000694370500001
WOS关键词	SOLID-PHASE EXTRACTION ; ON-VALVE FORMAT ; SEQUENTIAL INJECTION ; FLOW-INJECTION ; ACIDIC PHARMACEUTICALS ; MASS-SPECTROMETRY ; TRACE LEVELS ; WASTE-WATER ; MICROEXTRACTION ; NSAIDS
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/364194
作者单位	[Gemuh, Celestine Vubangsi; Horstkotte, Burkhard; Solich, Petr] Charles Univ Prague, Fac Pharm Hradec Kralove, Dept Analyt Chem, Akad Heyrovskeho 1203, Hradec Kralove 50005, Czech Republic
推荐引用方式 GB/T 7714	Gemuh, Celestine Vubangsi,Horstkotte, Burkhard,Solich, Petr. Lab-In-Syringe with Bead Injection Coupled Online to High-Performance Liquid Chromatography as Versatile Tool for Determination of Nonsteroidal Anti-Inflammatory Drugs in Surface Waters[J],2021,26(17).
APA	Gemuh, Celestine Vubangsi,Horstkotte, Burkhard,&Solich, Petr.(2021).Lab-In-Syringe with Bead Injection Coupled Online to High-Performance Liquid Chromatography as Versatile Tool for Determination of Nonsteroidal Anti-Inflammatory Drugs in Surface Waters.MOLECULES,26(17).
MLA	Gemuh, Celestine Vubangsi,et al."Lab-In-Syringe with Bead Injection Coupled Online to High-Performance Liquid Chromatography as Versatile Tool for Determination of Nonsteroidal Anti-Inflammatory Drugs in Surface Waters".MOLECULES 26.17(2021).