干旱区生态环境知识资源中心(Arid): Development and validation of a reliable LC-MS/MS method for simultaneous determination of deoxynivalenol and T-2 toxin in maize and oats

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DOI	10.1016/j.microc.2021.106599
	Development and validation of a reliable LC-MS/MS method for simultaneous determination of deoxynivalenol and T-2 toxin in maize and oats
	Tahoun, Ibrahim F.; Gab-Allah, Mohamed A.; Yamani, Randa N.; Shehata, Adel B.
通讯作者	Tahoun, IF ; Gab-Allah, MA (corresponding author), Natl Inst Stand, Reference Mat Lab, Tersa St,POB 136, Giza 12211, Egypt.
来源期刊	MICROCHEMICAL JOURNAL
ISSN	0026-265X
EISSN	1095-9149
出版年	2021
卷号	169
英文摘要	Herein, a new analytical method for sensitive, rapid and reliable determination of two major trichothecenes: deoxynivalenol (DON) and T-2 toxin (T2) in maize and oats has been successfully established and validated. Acetonitrile/water mixture was used for sample extraction, and two solid phase extraction clean-up routes (i.e., Oasis HLB cartridges and MycoSep 227 columns) were investigated for optimal recoveries and adequate clean-up efficiency. The investigated mycotoxins were separated by ultra-performance liquid chromatography (UPLC) system and quantified by triple quadrupole mass spectrometry (MS/MS) using an electro spray-ionization interface (ESI) operating in positive polarity. MRM experiments were optimized for each trichothecene to generate high sensitive transitions. The method was validated according to European Union Directive: 2002/657/EC. Matrix-matched calibration demonstrated nice linearity in the concentration range of 5-200 mu g/kg (T2) and 10-800 mu g/kg (DON) with a coefficient of determination (R-2) of 0.999. Fortified samples covering the same concentration ranges yielded average recovery values ranged from 85.0 to 95.3% with relative standard deviation (RSD) for the repeatability and reproducibility of <8%. Both mycotoxins exhibited limits of detection (0.04-0.12 mu g/kg) and quantification (0.13-0.38 mu g/kg) below the maximum limits approved by the European Union (EU). There was no significant ion suppression or enhancement for DON and T2 in both matrices since the matrix effect (ME %) varied between -11.6 to 12.9%. The proposed analytical method was successfully applied to maize and oat samples collected from local Egyptian markets. The key benefit of the current method is its ease of use, avoiding high consumption of solvents being therefore cost-effective, compatible with green analytical chemistry, and time-saving analytical approach.
英文关键词	Food safety Deoxynivalenol T-2 toxin Cereals UPLC-MS/MS Method validation
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000693074100006
WOS关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY ; B TRICHOTHECENES ; MASS-SPECTROMETRY ; MYCOTOXINS ; EXTRACTION ; PRODUCTS ; WHEAT ; ZEARALENONE ; CEREALS ; METABOLITES
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/364170
作者单位	[Tahoun, Ibrahim F.; Gab-Allah, Mohamed A.; Yamani, Randa N.] Natl Inst Stand, Reference Mat Lab, Tersa St,POB 136, Giza 12211, Egypt; [Shehata, Adel B.] Saudi Stand Metrol & Qual Org, Natl Measurement & Calibrat Ctr SASO NMCC, POB 3437, Riyadh 11471, Saudi Arabia
推荐引用方式 GB/T 7714	Tahoun, Ibrahim F.,Gab-Allah, Mohamed A.,Yamani, Randa N.,et al. Development and validation of a reliable LC-MS/MS method for simultaneous determination of deoxynivalenol and T-2 toxin in maize and oats[J],2021,169.
APA	Tahoun, Ibrahim F.,Gab-Allah, Mohamed A.,Yamani, Randa N.,&Shehata, Adel B..(2021).Development and validation of a reliable LC-MS/MS method for simultaneous determination of deoxynivalenol and T-2 toxin in maize and oats.MICROCHEMICAL JOURNAL,169.
MLA	Tahoun, Ibrahim F.,et al."Development and validation of a reliable LC-MS/MS method for simultaneous determination of deoxynivalenol and T-2 toxin in maize and oats".MICROCHEMICAL JOURNAL 169(2021).