干旱区生态环境知识资源中心(Arid): The Fodder Legume Chamaecytisus albidus Establishes Functional Symbiosis with Different Bradyrhizobial Symbiovars in Morocco

Arid

DOI	10.1007/s00248-021-01888-4
	The Fodder Legume Chamaecytisus albidus Establishes Functional Symbiosis with Different Bradyrhizobial Symbiovars in Morocco
	Bouhnik, Omar; Alami, Soufiane; Lamin, Hanane; Lamrabet, Mouad; Bennis, Meryeme; Ouajdi, Mohammed; Bellaka, Mhammed; Antri, Salwa El; Abbas, Younes; Abdelmoumen, Hanaa; Bedmar, Eulogio J.; Idrissi, Mustapha Missbah El
通讯作者	Bouhnik, O (corresponding author), Univ Mohammed V Rabat, Fac Sci, Ctr Biotechnol Vegetale & Microbienne Biodivers &, 4 Ave Ibn Battouta,BP 1014 RP, Rabat, Morocco.
来源期刊	MICROBIAL ECOLOGY
ISSN	0095-3628
EISSN	1432-184X
出版年	2021-10
英文摘要	In this work, we analyzed the symbiotic performance and diversity of rhizobial strains isolated from the endemic shrubby legume Chamaecytisus albidus grown in soils of three different agroforestry ecosystems representing arid and semi-arid forest areas in Morocco. The analysis of the rrs gene sequences from twenty-four representative strains selected after REP-PCR fingerprinting showed that all the strains belong to the genus Bradyrhizobium. Following multi-locus sequence analysis (MLSA) using the rrs, gyrB, recA, glnII, and rpoB housekeeping genes, five representative strains, CA20, CA61, CJ2, CB10, and CB61 were selected for further molecular studies. Phylogenetic analysis of the concatenated glnII, gyrB, recA, and rpoB genes showed that the strain CJ2 isolated from Sahel Doukkala soil is close to Bradyrhizobium canariense BTA-1( T) (96.95%); that strains CA20 and CA61 isolated from the Amhach site are more related to Bradyrhizobium valentinum LmjM3(T), with 96.40 and 94.57% similarity values; and that the strains CB10 and CB60 isolated from soil in the Bounaga site are more related to Bradyrhizobium murdochi CNPSo 4020( T) and Bradyrhizobium. retamae Ro19(T), with which they showed 95.45 and 97.34% similarity values, respectively. The phylogenetic analysis of the symbiotic genes showed that the strains belong to symbiovars lupini, genistearum, and retamae. All the five strains are able to nodulate Lupinus luteus, Retama monosperma, and Cytisus monspessilanus, but they do not nodulate Glycine max and Phaseolus vulgaris. The inoculation tests showed that the strains isolated from the 3 regions improve significantly the plant yield as compared to uninoculated plants. However, the strains of Bradyrhizobium sp. sv. retamae isolated from the site of Amhach were the most performing. The phenotypic analysis showed that the strains are able to use a wide range of carbohydrates and amino acids as sole carbon and nitrogen source. The strains isolated from the arid areas of Bounaga and Amhach were more tolerant to salinity and drought stress than strains isolated in the semi-arid area of Sahel Doukkala.
英文关键词	Bradyrhizobium Chamaecytisus albidus Diversity Symbiosis MLSA Phylogeny
类型	Article ; Early Access
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000705791300001
WOS关键词	PHASEOLUS-LUNATUS L. ; ROOT-NODULE BACTERIA ; SP-NOV. ; WESTERN-AUSTRALIA ; NODULATION GENES ; CANARY-ISLANDS ; REP-PCR ; STRAINS ; DIVERSITY ; RHIZOBIA
WOS类目	Ecology ; Marine & Freshwater Biology ; Microbiology
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/364168
作者单位	[Bouhnik, Omar; Alami, Soufiane; Lamin, Hanane; Lamrabet, Mouad; Bennis, Meryeme; Abdelmoumen, Hanaa; Idrissi, Mustapha Missbah El] Univ Mohammed V Rabat, Fac Sci, Ctr Biotechnol Vegetale & Microbienne Biodivers &, 4 Ave Ibn Battouta,BP 1014 RP, Rabat, Morocco; [Ouajdi, Mohammed; Bellaka, Mhammed; Antri, Salwa El] Ctr Rech Forestiere, Dept Eaux & Forets, Ave Omar Ibn El KhattabAgdal,BP 763, Rabat 10050, Morocco; [Abbas, Younes] Univ Sultan Moulay Slimane, Fac Polydiciplinaire, Beni Mellal, Morocco; [Bedmar, Eulogio J.] CSIC, Dept Microbiol Suelo & Sistemas Simbiot, Estn Expt Zaidin, Apartado Postal 419, Granada 18008, Spain
推荐引用方式 GB/T 7714	Bouhnik, Omar,Alami, Soufiane,Lamin, Hanane,et al. The Fodder Legume Chamaecytisus albidus Establishes Functional Symbiosis with Different Bradyrhizobial Symbiovars in Morocco[J],2021.
APA	Bouhnik, Omar.,Alami, Soufiane.,Lamin, Hanane.,Lamrabet, Mouad.,Bennis, Meryeme.,...&Idrissi, Mustapha Missbah El.(2021).The Fodder Legume Chamaecytisus albidus Establishes Functional Symbiosis with Different Bradyrhizobial Symbiovars in Morocco.MICROBIAL ECOLOGY.
MLA	Bouhnik, Omar,et al."The Fodder Legume Chamaecytisus albidus Establishes Functional Symbiosis with Different Bradyrhizobial Symbiovars in Morocco".MICROBIAL ECOLOGY (2021).