干旱区生态环境知识资源中心(Arid): Differential anti-inflammatory biomarkers of the desert truffles Terfezia claveryi and Tirmania nivea revealed via UPLC-QqQ-MS-based metabolomics combined to chemometrics

Arid

DOI	10.1016/j.lwt.2021.111965
	Differential anti-inflammatory biomarkers of the desert truffles Terfezia claveryi and Tirmania nivea revealed via UPLC-QqQ-MS-based metabolomics combined to chemometrics
	Darwish, Reham S.; Shawky, Eman; Nassar, Khallad M.; ElSayed, Ramez M. Rashad; Hussein, Dalia E.; Ghareeb, Doaa A.; Sohafy, Samah M. El
通讯作者	Shawky, E (corresponding author), Alexandria Univ, Fac Pharm, Dept Pharmacognosy, Alkhartoom Sq, Alexandria 21521, Egypt.
来源期刊	LWT-FOOD SCIENCE AND TECHNOLOGY
ISSN	0023-6438
EISSN	1096-1127
出版年	2021
卷号	150
英文摘要	Chemical profiling of white (Tirmania nivea) and black (Terfezia claveryi) desert truffles using UPLC-MS/MS analysis followed by their discrimination based on their anti-inflammatory biomarkers identified using chemometrics and network pharmacology-based analysis was attempted. 25 compounds were identified using UPLC/ MS/MS analysis in the extracts of T. claveryi and T. nivea belonging to saturated and unsaturated fatty acids, phenolic acids, alkanes and carboxylic acids. Identified compounds were forwarded to network-pharmacologybased analysis which revealed that resveratrol, oleic acid, margaric acid, naringenin, behenic acid and lauric acid were the top hit compounds related to anti-inflammatory targets. In-vitro anti-inflammatory testing assessed on LPS-stimulated WBCs revealed that T. claveryi and T. nivea extracts reduced TNF-alpha to the levels of 32 pg/ml and 55 pg/ml, IL-1 beta level to 55 and 85 pg/ml, and IFN-gamma level to 130 nmol/ml and 175 nmol/ml, respectively. Further, the constructed orthogonal projection to latent structures discriminant analysis (OPLS-DA) model coefficients indicated that T. claveryi exhibited its in-vitro anti-inflammatory activity through down regulation of TNF-alpha pro-inflammatory markers with margaric acid, oleic acid, naringenin and resveratrol being its biomarkers while T. nivea affected mainly IL-1 beta and IFN-gamma pro-inflammatory markers levels with behenic acid, lauric acid, caffeic acid and docosatetraenoic acid being its chemical markers.
英文关键词	Desert truffles Anti-inflammatory Biomarkers UPLC-QqQ-MS Metabolomics
类型	Article
语种	英语
开放获取类型	hybrid
收录类别	SCI-E
WOS记录号	WOS:000684179500011
WOS关键词	RESVERATROL ; FRAGMENTATION ; ANTIBACTERIAL ; SPECTROMETRY ; ACIDS
WOS类目	Food Science & Technology
WOS研究方向	Food Science & Technology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/364134
作者单位	[Darwish, Reham S.; Shawky, Eman; Sohafy, Samah M. El] Alexandria Univ, Fac Pharm, Dept Pharmacognosy, Alkhartoom Sq, Alexandria 21521, Egypt; [Nassar, Khallad M.; ElSayed, Ramez M. Rashad; Hussein, Dalia E.] Alexandria Univ, Fac Pharm, Alexandria, Egypt; [Ghareeb, Doaa A.] Alexandria Univ, Fac Sci, Biol Screening & Preclin Trial Lab, Dept Biochem, Alexandria, Egypt; [Ghareeb, Doaa A.] City Sci Res & Technol Applicat SRTA City, Pharmaceut & Fermentat Ind Dev Ctr, Alexandria, Egypt
推荐引用方式 GB/T 7714	Darwish, Reham S.,Shawky, Eman,Nassar, Khallad M.,et al. Differential anti-inflammatory biomarkers of the desert truffles Terfezia claveryi and Tirmania nivea revealed via UPLC-QqQ-MS-based metabolomics combined to chemometrics[J],2021,150.
APA	Darwish, Reham S..,Shawky, Eman.,Nassar, Khallad M..,ElSayed, Ramez M. Rashad.,Hussein, Dalia E..,...&Sohafy, Samah M. El.(2021).Differential anti-inflammatory biomarkers of the desert truffles Terfezia claveryi and Tirmania nivea revealed via UPLC-QqQ-MS-based metabolomics combined to chemometrics.LWT-FOOD SCIENCE AND TECHNOLOGY,150.
MLA	Darwish, Reham S.,et al."Differential anti-inflammatory biomarkers of the desert truffles Terfezia claveryi and Tirmania nivea revealed via UPLC-QqQ-MS-based metabolomics combined to chemometrics".LWT-FOOD SCIENCE AND TECHNOLOGY 150(2021).