干旱区生态环境知识资源中心(Arid): Identification of tumor antigens and immune subtypes in lower grade gliomas for mRNA vaccine development

Arid

DOI	10.1186/s12967-021-03014-x
	Identification of tumor antigens and immune subtypes in lower grade gliomas for mRNA vaccine development
	Ye, Liguo; Wang, Long; Yang, Ji'an; Hu, Ping; Zhang, Chunyu; Tong, Shi'ao; Liu, Zhennan; Tian, Daofeng
通讯作者	Tian, DF (corresponding author), Wuhan Univ, Renmin Hosp, Dept Neurosurg, Wuhan 430060, Hubei, Peoples R China.
来源期刊	JOURNAL OF TRANSLATIONAL MEDICINE
EISSN	1479-5876
出版年	2021
卷号	19 期号:1
英文摘要	Background As an important part of tumor immunotherapy for adjunct, therapeutic tumor vaccines have been effective against multiple solid cancers, while their efficacy against lower grade glioma (LGG) remains undefined. Immunophenotyping of tumors is an essential tool to evaluate the immune function of patients with immunodeficiency or autoimmunity. Therefore, this study aims to find the potential tumor antigen of LGG and identify the suitable population for cancer vaccination based on the immune landscape. Method The genomic and clinical data of 529 patients with LGG were obtained from TCGA, the mRNA_seq data of normal brain tissue were downloaded from GTEx. Differential expression gene and mutation analysis were performed to screen out potential antigens, K-M curves were carried out to investigate the correlation between the level of potential antigens and OS and DFS of patients. TIMER dataset was used to explore the correlation between genes and immune infiltrating cells. Immunophenotyping of 529 tumor samples was based on the single-sample gene sets enrichment analysis. Cibersort and Estimate algorithm were used to explore the tumor immune microenvironment characteristics in each immune subtype. Weighted gene co-expression network analysis (WGCNA) clustered immune-related genes and screened the hub genes, and pathway enrichment analyses were performed on the hub modules related to immune subtype in the WGCNA. Results Selecting for the mutated, up-regulated, prognosis- and immune-related genes, four potential tumor antigens were identified in LGG. They were also significantly positively associated with the antigen-presenting immune cells (APCs). Three robust immune subtypes, IS1, IS2 and IS3, represented immune status desert, immune inhibition, and inflamed respectively, which might serve as a predictive parameter. Subsequently, clinicopathological features, including the codeletion status of 1p19q, IDH mutation status, tumor mutation burden, tumor stemness, etc., were significantly different among subtypes. Conclusion FCGBP, FLNC, TLR7, and CSF2RA were potential antigens for developing cancer vaccination, and the patients in IS3 were considered the most suitable for vaccination in LGG.
英文关键词	Lower grade glioma Tumor antigens Immunotyping Cancer vaccination Bioinformatics
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000686660400002
WOS关键词	T-CELLS ; PROGRESSION
WOS类目	Medicine, Research & Experimental
WOS研究方向	Research & Experimental Medicine
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/364059
作者单位	[Ye, Liguo; Wang, Long; Yang, Ji'an; Hu, Ping; Zhang, Chunyu; Tong, Shi'ao; Liu, Zhennan; Tian, Daofeng] Wuhan Univ, Renmin Hosp, Dept Neurosurg, Wuhan 430060, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Ye, Liguo,Wang, Long,Yang, Ji'an,et al. Identification of tumor antigens and immune subtypes in lower grade gliomas for mRNA vaccine development[J],2021,19(1).
APA	Ye, Liguo.,Wang, Long.,Yang, Ji'an.,Hu, Ping.,Zhang, Chunyu.,...&Tian, Daofeng.(2021).Identification of tumor antigens and immune subtypes in lower grade gliomas for mRNA vaccine development.JOURNAL OF TRANSLATIONAL MEDICINE,19(1).
MLA	Ye, Liguo,et al."Identification of tumor antigens and immune subtypes in lower grade gliomas for mRNA vaccine development".JOURNAL OF TRANSLATIONAL MEDICINE 19.1(2021).