干旱区生态环境知识资源中心(Arid): RNA interference shows that Spook, the precursor gene of 20-hydroxyec-dysone (20E), regulates the molting of Macrobrachium nipponense

Arid

DOI	10.1016/j.jsbmb.2021.105976
	RNA interference shows that Spook, the precursor gene of 20-hydroxyec-dysone (20E), regulates the molting of Macrobrachium nipponense
	Yuan, Huwei; Qiao, Hui; Fu, Yin; Fu, Hongtuo; Zhang, Wenyi; Jin, Shubo; Gong, Yongsheng; Jiang, Sufei; Xiong, Yiwei; Hu, Yuning; Wu, Yan
通讯作者	Fu, HT (corresponding author), Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, 9 Shanshui East Rd, Wuxi 214081, Jiangsu, Peoples R China.
来源期刊	JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
ISSN	0960-0760
EISSN	1879-1220
出版年	2021
卷号	213
英文摘要	The aim of this study was to explore the function of the Mn-Spook gene, which was found in the ovary transcriptome of the Oriental river prawn (Macrobrachium nipponense). The Spook gene, which is the precursor gene of 20-hydroxyecdysone (20E), plays an important role in the process of molting in many arthropods, but its function in M. nipponense is unclear. We cloned the full-length Mn-Spook gene from the ovary of M. nipponense and found that it had the same conserved domains as the P450 gene of the Halloween family of genes. The MnSpook gene was highly expressed in ovary and gill tissue during the breeding period. During ovarian development, Mn-spook gene expression was highest at the nearly-ripe stage, and it also was highly expressed in the zoea developmental stage. Cellular localization analysis showed that Mn-Spook signals accumulated in the cytoplasmic membrane and nucleus of oocytes. Finally, we used RNA interference to evaluate the function of the Mn-Spook gene. Compared with the control group, in vivo injection of Mn-Spook dsRNA effectively downregulated the expression of Mn-Spook and the content of 20E. The molting frequency of M. nipponense in the experimental group also was significantly inhibited. These results demonstrated that the Mn-Spook gene played an important role in the molting process of M. nipponense.
英文关键词	Macrobrachium nipponense Mn-Spook 20E RNA interference Molt
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000704009000009
WOS关键词	HALLOWEEN GENES ; ECDYSTEROID BIOSYNTHESIS ; CYTOCHROME-P450 ENZYME ; ECDYSONE-RECEPTOR ; P450 ENZYMES ; DROSOPHILA-MELANOGASTER ; NUCLEAR RECEPTORS ; DESERT LOCUST ; SWIMMING CRAB ; BOMBYX-MORI
WOS类目	Biochemistry & Molecular Biology ; Endocrinology & Metabolism
WOS研究方向	Biochemistry & Molecular Biology ; Endocrinology & Metabolism
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/364038
作者单位	[Yuan, Huwei; Fu, Hongtuo] Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi 214081, Jiangsu, Peoples R China; [Qiao, Hui; Fu, Hongtuo; Zhang, Wenyi; Jin, Shubo; Gong, Yongsheng; Jiang, Sufei; Xiong, Yiwei; Hu, Yuning; Wu, Yan] Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Key Lab Freshwater Fisheries & Germplasm Resource, Minist Agr, Wuxi 214081, Jiangsu, Peoples R China; [Fu, Yin] Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China
推荐引用方式 GB/T 7714	Yuan, Huwei,Qiao, Hui,Fu, Yin,et al. RNA interference shows that Spook, the precursor gene of 20-hydroxyec-dysone (20E), regulates the molting of Macrobrachium nipponense[J],2021,213.
APA	Yuan, Huwei.,Qiao, Hui.,Fu, Yin.,Fu, Hongtuo.,Zhang, Wenyi.,...&Wu, Yan.(2021).RNA interference shows that Spook, the precursor gene of 20-hydroxyec-dysone (20E), regulates the molting of Macrobrachium nipponense.JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY,213.
MLA	Yuan, Huwei,et al."RNA interference shows that Spook, the precursor gene of 20-hydroxyec-dysone (20E), regulates the molting of Macrobrachium nipponense".JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY 213(2021).