干旱区生态环境知识资源中心(Arid): Structural Insight into Chromatin Recognition by Multiple Domains of the Tumor Suppressor RBBP1

Arid

DOI	10.1016/j.jmb.2021.167224
	Structural Insight into Chromatin Recognition by Multiple Domains of the Tumor Suppressor RBBP1
	Gong, Weibin; Liang, Qihui; Tong, Yufeng; Perrett, Sarah; Feng, Yingang
通讯作者	Perrett, S (corresponding author), Chinese Acad Sci, CAS Ctr Excellence Biomacromol, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China. ; Feng, YG (corresponding author), Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biofuels, Qingdao 266101, Peoples R China.
来源期刊	JOURNAL OF MOLECULAR BIOLOGY
ISSN	0022-2836
EISSN	1089-8638
出版年	2021
卷号	433 期号:21
英文摘要	Retinoblastoma-binding protein 1 (RBBP1) is involved in gene regulation, epigenetic regulation, and disease processes. RBBP1 contains five domains with DNA-binding or histone-binding activities, but how RBBP1 specifically recognizes chromatin is still unknown. An AT-rich interaction domain (ARID) in RBBP1 was proposed to be the key region for DNA-binding and gene suppression. Here, we first determined the solution structure of a tandem PWWP-ARID domain mutant of RBBP1 after deletion of a long flexible acidic loop L12 in the ARID domain. NMR titration results indicated that the ARID domain interacts with DNA with no GC- or AT-rich preference. Surprisingly, we found that the loop L12 binds to the DNA-binding region of the ARID domain as a DNA mimic and inhibits DNA binding. The loop L12 can also bind weakly to the Tudor and chromobarrel domains of RBBP1, but binds more strongly to the DNA-binding region of the histone H2A-H2B heterodimer. Furthermore, both the loop L12 and DNA can enhance the binding of the chromobarrel domain to H3K4me3 and H4K20me3. Based on these results, we propose a model of chromatin recognition by RBBP1, which highlights the unexpected multiple key roles of the disordered acidic loop L12 in the specific binding of RBBP1 to chromatin. (C) 2021 Elsevier Ltd. All rights reserved.
英文关键词	retinoblastoma-binding protein 1 DNA binding disordered loop autoinhibition nuclear magnetic resonance
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000708675600001
WOS关键词	TRANSCRIPTIONAL REPRESSION ; RBP1 RECRUITS ; FAMILY ; IDENTIFICATION ; ARID4A ; REVEALS ; REGIONS ; COMPLEX
WOS类目	Biochemistry & Molecular Biology
WOS研究方向	Biochemistry & Molecular Biology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/363967
作者单位	[Gong, Weibin; Liang, Qihui; Perrett, Sarah] Chinese Acad Sci, CAS Ctr Excellence Biomacromol, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China; [Feng, Yingang] Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biofuels, Qingdao 266101, Peoples R China; [Feng, Yingang] Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Synthet Biol, Qingdao 266101, Peoples R China; [Tong, Yufeng] Univ Windsor, Dept Chem & Biochem, Windsor, ON N9B 3P4, Canada; [Liang, Qihui; Perrett, Sarah; Feng, Yingang] Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Gong, Weibin,Liang, Qihui,Tong, Yufeng,et al. Structural Insight into Chromatin Recognition by Multiple Domains of the Tumor Suppressor RBBP1[J],2021,433(21).
APA	Gong, Weibin,Liang, Qihui,Tong, Yufeng,Perrett, Sarah,&Feng, Yingang.(2021).Structural Insight into Chromatin Recognition by Multiple Domains of the Tumor Suppressor RBBP1.JOURNAL OF MOLECULAR BIOLOGY,433(21).
MLA	Gong, Weibin,et al."Structural Insight into Chromatin Recognition by Multiple Domains of the Tumor Suppressor RBBP1".JOURNAL OF MOLECULAR BIOLOGY 433.21(2021).