干旱区生态环境知识资源中心(Arid): Diversity, Phylogeny and Antagonistic Activity of Fungal Endophytes Associated with Endemic Species of Cycas (Cycadales) in China

Arid

DOI	10.3390/jof7070572
	Diversity, Phylogeny and Antagonistic Activity of Fungal Endophytes Associated with Endemic Species of Cycas (Cycadales) in China
	Pecundo, Melissa H.; dela Cruz, Thomas Edison E.; Chen, Tao; Notarte, Kin Israel; Ren, Hai; Li, Nan
通讯作者	Li, N (corresponding author), Chinese Acad Sci, South China Bot Garden, Guangzhou 510650, Peoples R China. ; Li, N (corresponding author), Chinese Acad Sci, Fairy Lake Bot Garden, Shenzhen 518004, Peoples R China. ; Li, N (corresponding author), Univ Chinese Acad Sci, Beijing 100049, Peoples R China.
来源期刊	JOURNAL OF FUNGI
EISSN	2309-608X
出版年	2021
卷号	7 期号:7
英文摘要	The culture-based approach was used to characterize the fungal endophytes associated with the coralloid roots of the endemic Cycas debaoensis and Cycas fairylakea from various population sites in China. We aim to determine if the assemblages of fungal endophytes inside these endemic plant hosts are distinct and could be explored for bioprospecting. The isolation method yielded a total of 284 culturable fungal strains. Identification based on the analysis of the internal transcribed spacer (ITS) rDNA showed that they belonged to two phyla, five classes, eight orders and 22 families. At least 33 known genera and 62 different species were confirmed based on >97% ITS sequence similarity. The most frequent and observed core taxa in the two host species regardless of their population origin were Talaromyces, Penicillium, Fusarium, Pochonia and Gliocladiopsis. Seventy percent was a rare component of the fungal communities with only one or two recorded isolates. Contrary to common notions, diversity and fungal richness were significantly higher in C. debaoensis and C. fairylakea collected from a botanical garden, while the lowest was observed in C. debaoensis from a natural habitat; this provides evidence that garden management, and to a minor extent, ex-situ conservation practice, could influence fungal endophyte communities. We further selected nineteen fungal isolates and screened for their antagonistic activities via a co-cultivation approach against the phytopathogens, Diaporthe sp. and Colletotrichum sp. Among these, five isolates with high ITS similarity matches with Hypoxylon vinosupulvinatum (GD019, 99.61%), Penicillium sp. (BD022, 100%), Penicillifer diparietisporus (GD008, 99.46%), Clonostachys rogersoniana (BF024, 99.46%) and C. rosea (BF011, 99.1%), which showed exceptional antagonistic activities against the phytopathogenic fungi with a significant inhibition rate of 70-80%. Taken together, our data presented the first and most comprehensive molecular work on culturable fungal endophytes associated with the coralloid roots of cycads. Our study also demonstrated that about 5% of fungal endophytes were not detected by the high-throughput sequencing approach, implying the equal importance of a culture-dependent approach to study fungal communities of cycads. We further highlighted the potential role of endemic and rare plants to discover and isolate unique plant-associated fungal taxa with excellent biocontrol properties.
英文关键词	antifungal activity co-cultivation assay coralloid roots fungal diversity host habitat
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000676685300001
WOS关键词	ROOT ENDOPHYTES ; HOST GENOTYPE ; COMMUNITIES ; PLANTS ; THERMOTOLERANCE ; COLLETOTRICHUM ; ANTIBACTERIAL ; METABOLITES ; DESERT ; GROWTH
WOS类目	Microbiology ; Mycology
WOS研究方向	Microbiology ; Mycology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/363880
作者单位	[Pecundo, Melissa H.; Ren, Hai; Li, Nan] Chinese Acad Sci, South China Bot Garden, Guangzhou 510650, Peoples R China; [Pecundo, Melissa H.; Chen, Tao; Li, Nan] Chinese Acad Sci, Fairy Lake Bot Garden, Shenzhen 518004, Peoples R China; [Pecundo, Melissa H.; Chen, Tao; Ren, Hai; Li, Nan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [dela Cruz, Thomas Edison E.] Univ Santo Tomas, Dept Biol Sci, Coll Sci, Manila 1008, Philippines; [dela Cruz, Thomas Edison E.; Notarte, Kin Israel] Univ Santo Tomas, Res Ctr Nat & Appl Sci, Fungal Biodivers Ecogen & Systemat FBeS Grp, Manila 1008, Philippines
推荐引用方式 GB/T 7714	Pecundo, Melissa H.,dela Cruz, Thomas Edison E.,Chen, Tao,et al. Diversity, Phylogeny and Antagonistic Activity of Fungal Endophytes Associated with Endemic Species of Cycas (Cycadales) in China[J],2021,7(7).
APA	Pecundo, Melissa H.,dela Cruz, Thomas Edison E.,Chen, Tao,Notarte, Kin Israel,Ren, Hai,&Li, Nan.(2021).Diversity, Phylogeny and Antagonistic Activity of Fungal Endophytes Associated with Endemic Species of Cycas (Cycadales) in China.JOURNAL OF FUNGI,7(7).
MLA	Pecundo, Melissa H.,et al."Diversity, Phylogeny and Antagonistic Activity of Fungal Endophytes Associated with Endemic Species of Cycas (Cycadales) in China".JOURNAL OF FUNGI 7.7(2021).