干旱区生态环境知识资源中心(Arid): Development and validation of an automated solid-phase extraction-LC-MS/MS method for the bioanalysis of fluoxetine in human plasma

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DOI	10.4103/japtr.JAPTR_308_20
	Development and validation of an automated solid-phase extraction-LC-MS/MS method for the bioanalysis of fluoxetine in human plasma
	Ishtiaque Ahmad, Zabih Ullah; Khan, Mohd Ibrahim; Alahmari, Abdullah Khaloofa; Khan, Mohd Faiyaz
通讯作者	Khan, MF (corresponding author), Prince Sattam Bin Abdulaziz Univ, Coll Pharm, Dept Clin Pharm, Riyadh, Saudi Arabia.
来源期刊	JOURNAL OF ADVANCED PHARMACEUTICAL TECHNOLOGY & RESEARCH
ISSN	2231-4040
EISSN	0976-2094
出版年	2021
卷号	12 期号:3 页码:267-273
英文摘要	A wide-range, specific, and precise liquid chromatography tandem mass spectrometric (LC-MS/MS) technique for quantifying fluoxetine (FLX) in human plasma was developed using the RapidTracer (R) automated solid-phase extraction (SPE) method; the analyte and internal standard (IS) were extricated on Oasis MCX SPE cartridges. Acetonitrile and 5 mM ammonium formate buffer (90:10 v/v) were used as mobile phase to achieve chromatographic separation on the reverse phase (C-18 column). The analyte and IS were ionized using +ve electrospray ionization approach which was further traced by multiple-reaction monitoring on a tandem mass spectrometer. To quantify the FLX and FLX-d5, the parent-to-daughter ion transition of m/z of 310.0/44,1 and 315.0/44.0 was used, respectively. The method demonstrated a linear active limit of 0.20-30 ng/ml with recoveries ranging from 63.04% to 79.39% for quality control samples and 61,25% for IS samples. The concentrations over the calibration range demonstrated acceptable precision and accuracy. Due to the high inconsistency of the FLX concentration data, the minimum threshold of the assay was kept at 0.20 ng/ml. The flow rate was maintained at 500 mu L/min, and the time for sample analysis for each injection was 3.5 min. The method was found to be specific, sensitive, and faster with minimum utilization of organic solvents and was utilized further for metabolic and pharmacokinetic studies.
英文关键词	Automation fluoxetine human plasma mass spectrometry solid-phase extraction
类型	Article
语种	英语
收录类别	ESCI
WOS记录号	WOS:000675444600010
WOS关键词	NORFLUOXETINE ; MICROEXTRACTION ; METABOLITES ; OLANZAPINE
WOS类目	Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/363703
作者单位	Prince Sultan Mil Med City, Sci Res Ctr, Riyadh, Saudi Arabia; [Alahmari, Abdullah Khaloofa; Khan, Mohd Faiyaz] Prince Sattam Bin Abdulaziz Univ, Coll Pharm, Dept Clin Pharm, Riyadh, Saudi Arabia; [Ishtiaque Ahmad, Zabih Ullah] Buraydah Coll, Coll Pharm & Dent, Dept Pharmaceut Sci, Alqassim, Saudi Arabia; [Khan, Mohd Ibrahim] Sam Higginbottom Univ Agr Technol & Sci, Sci SIHAS, Dept Pharmaceut, Naini, Prayagraj, India
推荐引用方式 GB/T 7714	Ishtiaque Ahmad, Zabih Ullah,Khan, Mohd Ibrahim,Alahmari, Abdullah Khaloofa,et al. Development and validation of an automated solid-phase extraction-LC-MS/MS method for the bioanalysis of fluoxetine in human plasma[J],2021,12(3):267-273.
APA	Ishtiaque Ahmad, Zabih Ullah,Khan, Mohd Ibrahim,Alahmari, Abdullah Khaloofa,&Khan, Mohd Faiyaz.(2021).Development and validation of an automated solid-phase extraction-LC-MS/MS method for the bioanalysis of fluoxetine in human plasma.JOURNAL OF ADVANCED PHARMACEUTICAL TECHNOLOGY & RESEARCH,12(3),267-273.
MLA	Ishtiaque Ahmad, Zabih Ullah,et al."Development and validation of an automated solid-phase extraction-LC-MS/MS method for the bioanalysis of fluoxetine in human plasma".JOURNAL OF ADVANCED PHARMACEUTICAL TECHNOLOGY & RESEARCH 12.3(2021):267-273.