干旱区生态环境知识资源中心(Arid): Biosynthetic potential of uncultured Antarctic soil bacteria revealed through long-read metagenomic sequencing

Arid

DOI	10.1038/s41396-021-01052-3
	Biosynthetic potential of uncultured Antarctic soil bacteria revealed through long-read metagenomic sequencing
	Waschulin, Valentin; Borsetto, Chiara; James, Robert; Newsham, Kevin K.; Donadio, Stefano; Corre, Christophe; Wellington, Elizabeth
通讯作者	Waschulin, V (corresponding author), Univ Warwick, Sch Life Sci, Coventry, W Midlands, England.
来源期刊	ISME JOURNAL
ISSN	1751-7362
EISSN	1751-7370
出版年	2021-07
英文摘要	The growing problem of antibiotic resistance has led to the exploration of uncultured bacteria as potential sources of new antimicrobials. PCR amplicon analyses and short-read sequencing studies of samples from different environments have reported evidence of high biosynthetic gene cluster (BGC) diversity in metagenomes, indicating their potential for producing novel and useful compounds. However, recovering full-length BGC sequences from uncultivated bacteria remains a challenge due to the technological restraints of short-read sequencing, thus making assessment of BGC diversity difficult. Here, long-read sequencing and genome mining were used to recover >1400 mostly full-length BGCs that demonstrate the rich diversity of BGCs from uncultivated lineages present in soil from Mars Oasis, Antarctica. A large number of highly divergent BGCs were not only found in the phyla Acidobacteriota, Verrucomicrobiota and Gemmatimonadota but also in the actinobacterial classes Acidimicrobiia and Thermoleophilia and the gammaproteobacterial order UBA7966. The latter furthermore contained a potential novel family of RiPPs. Our findings underline the biosynthetic potential of underexplored phyla as well as unexplored lineages within seemingly well-studied producer phyla. They also showcase long-read metagenomic sequencing as a promising way to access the untapped genetic reservoir of specialised metabolite gene clusters of the uncultured majority of microbes.
类型	Article ; Early Access
语种	英语
开放获取类型	Green Published, hybrid, Green Accepted
收录类别	SCI-E
WOS记录号	WOS:000672250300001
WOS关键词	NATURAL-PRODUCTS ; GENOME ; DIVERSITY ; DISCOVERY ; ALIGNMENT ; COVERAGE
WOS类目	Ecology ; Microbiology
WOS研究方向	Environmental Sciences & Ecology ; Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/363687
作者单位	[Waschulin, Valentin; Borsetto, Chiara; Corre, Christophe; Wellington, Elizabeth] Univ Warwick, Sch Life Sci, Coventry, W Midlands, England; [James, Robert] Quadram Inst, Norwich, Norfolk, England; [Newsham, Kevin K.] NERC British Antarctic Survey, Cambridge, England; [Donadio, Stefano] NAICONS Srl, Milan, Italy; [Corre, Christophe] Univ Warwick, Dept Chem, Coventry, W Midlands, England
推荐引用方式 GB/T 7714	Waschulin, Valentin,Borsetto, Chiara,James, Robert,et al. Biosynthetic potential of uncultured Antarctic soil bacteria revealed through long-read metagenomic sequencing[J],2021.
APA	Waschulin, Valentin.,Borsetto, Chiara.,James, Robert.,Newsham, Kevin K..,Donadio, Stefano.,...&Wellington, Elizabeth.(2021).Biosynthetic potential of uncultured Antarctic soil bacteria revealed through long-read metagenomic sequencing.ISME JOURNAL.
MLA	Waschulin, Valentin,et al."Biosynthetic potential of uncultured Antarctic soil bacteria revealed through long-read metagenomic sequencing".ISME JOURNAL (2021).