干旱区生态环境知识资源中心(Arid): Expression of the Malus sieversii NF-YB21 Encoded Gene Confers Tolerance to Osmotic Stresses in Arabidopsis thaliana

Arid

DOI	10.3390/ijms22189777
	Expression of the Malus sieversii NF-YB21 Encoded Gene Confers Tolerance to Osmotic Stresses in Arabidopsis thaliana
	Feng, Chen; Wang, Yanyan; Sun, Yueting; Peng, Xiang; Zhang, Xiang; Zhou, Xin; Jiao, Jiale; Zhai, Zefeng; Xiao, Yuqin; Wang, Weili; Liu, Yang; Li, Tianhong
通讯作者	Liu, Y ; Li, TH (corresponding author), China Agr Univ, Coll Hort, Dept Pomol, State Key Labs Agrobiotechnol, Beijing 100193, Peoples R China.
来源期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
EISSN	1422-0067
出版年	2021
卷号	22 期号:18
英文摘要	Drought is the main environmental factor that limits the yield and quality of apples (Malus x domestica) grown in arid and semi-arid regions. Nuclear factor Ys (NF-Ys) are important transcription factors involved in the regulation of plant growth, development, and various stress responses. However, the function of NF-Y genes is poorly understood in apples. Here, we identified 43 NF-Y genes in the genome of apples and conducted an initial functional characterization of the apple NF-Y. Expression analysis of NF-Y members in M. sieversii revealed that a large number of NF-Ys were highly expressed in the roots compared with the leaves, and a large proportion of NF-Y genes responded to drought treatment. Furthermore, heterologous expression of MsNF-YB21, which was significantly upregulated by drought, led to a longer root length and, thus, conferred improved osmotic and salt tolerance in Arabidopsis. Moreover, the physiological analysis of MsNF-YB21 overexpression revealed enhanced antioxidant systems, including antioxidant enzymes and compatible solutes. In addition, genes encoding catalase (AtCAT2, AtCAT3), superoxide dismutase (AtFSD1, AtFSD3, AtCSD1), and peroxidase (AtPER12, AtPER42, AtPER47, AtPER51) showed upregulated expression in the MsNF-YB21 overexpression lines. These results for the MsNF-Y gene family provide useful information for future studies on NF-Ys in apples, and the functional analysis of MsNF-YB21 supports it as a potential target in the improvement of apple drought tolerance via biotechnological strategies.
英文关键词	Malus sieversii nuclear factor Y MsNF-YB21 osmotic stress antioxidant gene expression
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000700557800001
WOS关键词	NUCLEAR-FACTOR-Y ; GENOME-WIDE IDENTIFICATION ; TRANSCRIPTION FACTOR ; DROUGHT STRESS ; OVEREXPRESSION ; ROLES ; RESPONSES ; SALINITY ; COMPLEX ; NF-YC9
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
来源机构	中国农业大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/363652
作者单位	[Feng, Chen; Wang, Yanyan; Sun, Yueting; Peng, Xiang; Zhang, Xiang; Zhou, Xin; Jiao, Jiale; Zhai, Zefeng; Xiao, Yuqin; Wang, Weili; Liu, Yang; Li, Tianhong] China Agr Univ, Coll Hort, Dept Pomol, State Key Labs Agrobiotechnol, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Feng, Chen,Wang, Yanyan,Sun, Yueting,et al. Expression of the Malus sieversii NF-YB21 Encoded Gene Confers Tolerance to Osmotic Stresses in Arabidopsis thaliana[J]. 中国农业大学,2021,22(18).
APA	Feng, Chen.,Wang, Yanyan.,Sun, Yueting.,Peng, Xiang.,Zhang, Xiang.,...&Li, Tianhong.(2021).Expression of the Malus sieversii NF-YB21 Encoded Gene Confers Tolerance to Osmotic Stresses in Arabidopsis thaliana.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,22(18).
MLA	Feng, Chen,et al."Expression of the Malus sieversii NF-YB21 Encoded Gene Confers Tolerance to Osmotic Stresses in Arabidopsis thaliana".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22.18(2021).