干旱区生态环境知识资源中心(Arid): Identification of the Short Neuropeptide F and Short Neuropeptide F Receptor Genes and Their Roles of Food Intake in Dendroctonus armandi

Arid

DOI	10.3390/insects12090844
	Identification of the Short Neuropeptide F and Short Neuropeptide F Receptor Genes and Their Roles of Food Intake in Dendroctonus armandi
	Liu, Bin; Fu, Danyang; Ning, Hang; Tang, Ming; Chen, Hui
通讯作者	Tang, M ; Chen, H (corresponding author), Northwest A&F Univ, Coll Forestry, Xianyang 712100, Peoples R China. ; Tang, M ; Chen, H (corresponding author), South China Agr Univ, Coll Forestry & Landscape Architecture, State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangdong Key Lab Innovat Dev & Utilizat Forest P, Guangzhou 510642, Peoples R China.
来源期刊	INSECTS
EISSN	2075-4450
出版年	2021
卷号	12 期号:9
英文摘要	Simple Summary The Chinese white pine beetle, Dendroctonus armandi, is a destructive pest of coniferous forests in the middle Qinling Mountains of China. The short neuropeptide F (sNPF) and short NPF receptor (sNPFR) genes have important roles in a broad range of physiological and behavioral processes. However, the function of sNPF signaling pathway in regulating the food intake of Dendroctonus armandi has been unclear. In this study, we cloned and characterized cDNAs encoding sNPF and sNPF receptor in D. armandi and analyzed their spatiotemporal expression patterns, as well as changes in expression after starvation. Addtionally, sNPF and sNPFR knockdown in beetles using RNA interference significantly increased mortality and reduced their food intake and body weight through changes of a biosynthesis and metabolism pathway. From these results, we conclude that sNPF signaling pathway is important in the feeding control of D. armandi. The short neuropeptide F (sNPF) is an essential signaling molecule that is evolutionarily conserved and involved in a broad range of physiological functions in the invertebrates, by interacting with sNPF receptors, which belong to G protein-coupled receptors (GPCR). However, the function of sNPF in regulating the food intake of Dendroctonus armandi has been unclear. In this study, we cloned and characterized cDNAs encoding sNPF and sNPF receptor in the D. armandi and made bioinformatics predictions on the deduced amino acid sequences. They had a high degree of similarity to that of Dendroctonus ponderosa. Quantitative real-time reverse transcription PCR (qRT-PCR) revealed that the transcript levels of both sNPF and sNPFR varied across developmental stages and body parts. In addition, the sNPF and sNPFR expression levels were upregulated in starved beetles, and the expression levels recovered after re-feeding. Furthermore, RNAi knockdown by the injection of sNPF and sNPFR dsRNA into beetles significantly increased mortality and reduced their food intake and body weight, and also caused decrease of glycogen and free fatty acid and increase of trehalose. These results indicate that sNPF signaling pathway plays an important role in the regulation of food intake and provides a potential molecular target for the eco-friendly control strategies of this pest.
英文关键词	Dendroctonus armandi short neuropeptide F short neuropeptide F receptor food intake molecular target RNA interference
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000700689700001
WOS关键词	CHINESE WHITE-PINE ; CURCULIONIDAE SCOLYTINAE ; TRANSCRIPT LEVELS ; DESERT LOCUST ; PEPTIDE ; EXPRESSION ; RESPONSES ; NEURONS ; BEETLE ; COMPONENTS
WOS类目	Entomology
WOS研究方向	Entomology
来源机构	西北农林科技大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/363598
作者单位	[Liu, Bin; Fu, Danyang; Ning, Hang; Tang, Ming; Chen, Hui] Northwest A&F Univ, Coll Forestry, Xianyang 712100, Peoples R China; [Tang, Ming; Chen, Hui] South China Agr Univ, Coll Forestry & Landscape Architecture, State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangdong Key Lab Innovat Dev & Utilizat Forest P, Guangzhou 510642, Peoples R China
推荐引用方式 GB/T 7714	Liu, Bin,Fu, Danyang,Ning, Hang,et al. Identification of the Short Neuropeptide F and Short Neuropeptide F Receptor Genes and Their Roles of Food Intake in Dendroctonus armandi[J]. 西北农林科技大学,2021,12(9).
APA	Liu, Bin,Fu, Danyang,Ning, Hang,Tang, Ming,&Chen, Hui.(2021).Identification of the Short Neuropeptide F and Short Neuropeptide F Receptor Genes and Their Roles of Food Intake in Dendroctonus armandi.INSECTS,12(9).
MLA	Liu, Bin,et al."Identification of the Short Neuropeptide F and Short Neuropeptide F Receptor Genes and Their Roles of Food Intake in Dendroctonus armandi".INSECTS 12.9(2021).