干旱区生态环境知识资源中心(Arid): Genome-Wide Identification of Neuropeptides and Their Receptors in an Aphid Endoparasitoid Wasp, Aphidius gifuensi

Arid

DOI	10.3390/insects12080745
	Genome-Wide Identification of Neuropeptides and Their Receptors in an Aphid Endoparasitoid Wasp, Aphidius gifuensi
	Kong, Xue; Li, Zhen-Xiang; Gao, Yu-Qing; Liu, Fang-Hua; Chen, Zhen-Zhen; Tian, Hong-Gang; Liu, Tong-Xian; Xu, Yong-Yu; Kang, Zhi-Wei
通讯作者	Xu, YY ; Kang, ZW (corresponding author), Shandong Agr Univ, Coll Plant Protect, Tai An 271018, Shandong, Peoples R China. ; Kang, ZW (corresponding author), Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Key Lab Northwest Loess Plateau Crop Pest Managem, Minist Agr, Yangling 712100, Shaanxi, Peoples R China.
来源期刊	INSECTS
EISSN	2075-4450
出版年	2021
卷号	12 期号:8
英文摘要	Simple Summary Neuropeptides and their receptors play important roles in insect physiology and behavior. Due to their specificity and importance, they have been considered as the targets for new environmentally friendly insecticides. Hence, more information on genomics and transcriptomics of neuropeptide and their receptors in more insects, especially in beneficial insects, are needed to avoid the side effects of these environmentally friendly insecticides. Aphidius gifuensis is one of the most well-known aphid parasitoids and has been successfully used to control aphids such as Myzus persicae and Sitobion avenae. In this study, we systematically identified neuropeptides and their receptors from the genome and head transcriptome of A. gifuensis. Meanwhile, we also analyzed their expression patterns in response to imidacloprid exposure and in different tissues. Our results not only laid a preliminary foundation for functional studies on neuropeptide precursors and their receptors but also provided useful information for future pesticide development. In insects, neuropeptides and their receptors not only play a critical role in insect physiology and behavior but also are the potential targets for novel pesticide discoveries. Aphidius gifuensis is one of the most important and widespread aphid parasitoids, and has been successfully used to control aphid. In the present work, we systematically identified neuropeptides and their receptors from the genome and head transcriptome of A. gifuensis. A total of 35 neuropeptide precursors and 49 corresponding receptors were identified. The phylogenetic analyses demonstrated that 35 of these receptors belong to family-A, four belong to family-B, two belong to leucine-rich repeat-containing GPCRs, four belong to receptor guanylyl cyclases, and four belong to receptor tyrosine kinases. Oral ingestion of imidacloprid significantly up-regulated five neuropeptide precursors and four receptors whereas three neuropeptide precursors and eight receptors were significantly down-regulated, which indicated that these neuropeptides and their receptors are potential targets of some commercial insecticides. The RT-qPCR results showed that dopamine receptor 1, dopamine receptor 2, octopamine receptor, allatostatin-A receptor, neuropeptides capa receptor, SIFamide receptor, FMRFamide receptor, tyramine receptor and short neuropeptide F predominantly were expressed in the head whilst the expression of ion transport peptide showed widespread distribution in various tissues. The high expression levels of these genes suggest their important roles in the central nervous system. Taken together, our study provides fundamental information that may further our understanding of neuropeptidergic signaling systems in the regulation of the physiology and behavior of solitary wasps. Furthermore, this information could also aid in the design and discovery of specific and environment-friendly insecticides.
英文关键词	Aphidius gifuensis neuropeptide precursors neuropeptide receptors expression profile
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000690620500001
WOS关键词	PROTEIN-COUPLED RECEPTORS ; HYMENOPTERA-BRACONIDAE ; TYROSINE KINASE ; MYZUS-PERSICAE ; DESERT LOCUST ; FRUIT-FLY ; DROSOPHILA ; PEPTIDES ; BEHAVIOR ; GPCRS
WOS类目	Entomology
WOS研究方向	Entomology
来源机构	西北农林科技大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/363596
作者单位	[Kong, Xue; Li, Zhen-Xiang; Gao, Yu-Qing; Liu, Fang-Hua; Chen, Zhen-Zhen; Xu, Yong-Yu; Kang, Zhi-Wei] Shandong Agr Univ, Coll Plant Protect, Tai An 271018, Shandong, Peoples R China; [Tian, Hong-Gang; Kang, Zhi-Wei] Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Key Lab Northwest Loess Plateau Crop Pest Managem, Minist Agr, Yangling 712100, Shaanxi, Peoples R China; [Liu, Tong-Xian] Qingdao Agr Univ, Coll Plant Hlth & Med, Key Lab Integrated Crop Pest Management Shandong, Qingdao 266109, Peoples R China
推荐引用方式 GB/T 7714	Kong, Xue,Li, Zhen-Xiang,Gao, Yu-Qing,et al. Genome-Wide Identification of Neuropeptides and Their Receptors in an Aphid Endoparasitoid Wasp, Aphidius gifuensi[J]. 西北农林科技大学,2021,12(8).
APA	Kong, Xue.,Li, Zhen-Xiang.,Gao, Yu-Qing.,Liu, Fang-Hua.,Chen, Zhen-Zhen.,...&Kang, Zhi-Wei.(2021).Genome-Wide Identification of Neuropeptides and Their Receptors in an Aphid Endoparasitoid Wasp, Aphidius gifuensi.INSECTS,12(8).
MLA	Kong, Xue,et al."Genome-Wide Identification of Neuropeptides and Their Receptors in an Aphid Endoparasitoid Wasp, Aphidius gifuensi".INSECTS 12.8(2021).