干旱区生态环境知识资源中心(Arid): Double-Stranded RNA-Degrading Enzymes Reduce the Efficiency of RNA Interference in Plutella xylostella

Arid

DOI	10.3390/insects12080712
	Double-Stranded RNA-Degrading Enzymes Reduce the Efficiency of RNA Interference in Plutella xylostella
	Chen, Jin-Zhi; Jiang, Ying-Xia; Li, Miao-Wen; Li, Jian-Wen; Zha, Ben-Hu; Yang, Guang
通讯作者	Yang, G (corresponding author), Fujian Agr & Forestry Univ, Inst Appl Ecol, State Key Lab Ecol Pest Control Fujian & Taiwan C, Fuzhou 350002, Peoples R China. ; Yang, G (corresponding author), Minist Educ, Joint Int Res Lab Ecol Pest Control, Fuzhou 350002, Peoples R China. ; Yang, G (corresponding author), Minist Agr & Rural Affairs, Key Lab Integrated Pest Management Fujian Taiwan, Fuzhou 350002, Peoples R China. ; Yang, G (corresponding author), Fujian Prov Univ, Key Lab Green Control Insect Pests, Fujian Agr & Forestry Univ, Fuzhou 350002, Peoples R China. ; Yang, G (corresponding author), Fujian Agr & Forestry Univ, Ministerial & Prov Joint Innovat Ctr Safety Prod, Fuzhou 350002, Peoples R China. ; Yang, G (corresponding author), Fujian Agr & Forestry Univ, Coll Plant Protect, 15 Shangxiadian Rd, Fuzhou 350002, Peoples R China.
来源期刊	INSECTS
EISSN	2075-4450
出版年	2021
卷号	12 期号:8
英文摘要	Simple Summary The efficiency of Lepidoptera RNA interference (RNAi) is highly varied among different species, different periods, and different genes. The stability of dsRNA is one of the important factors. DsRNA-degrading enzymes (dsRNases) are the key factors affecting the stability of dsRNA in insects. The efficiency of RNAi in diamondback moths was low and unstable. Furthermore, in vitro experiments, we found that dsRNA was completely degraded when incubated with the hemolymph or gut fluid of diamondback moths. Therefore, we hypothesized that the efficiency of RNAi in diamondback moths was decreased predominantly due to degradation of dsRNA by dsRNase. In this study, we identified four dsRNases in diamondback moths: PxdsRNase1 was mainly expressed in the hemolymph; and PxdsRNase2 and PxdsRNase3 were mainly expressed in the intestinal tract. PxdsRNase1, PxdsRNase2, and PxdsRNase3 were verified to be involved in the RNAi process in diamondback moths. In vitro, the recombinant protein of PxdsRNase1 degraded dsRNA completely and PxdsRNase3 cleaved dsRNA without complete degradation. Overall, our findings provided a fundamental basis for understanding the mechanism of dsRNase involvement in the RNAi process and using RNAi to control diamondback moths in the future. DsRNA-degrading enzymes (dsRNases) have been recognized as important factors in reducing RNA interference (RNAi) efficiency in different insect species. However, dsRNases in Plutella xylostella are still unknown. We identified the full-length cDNAs of PxdsRNase1, PxdsRNase2, PxdsRNase3, and PxdsRNase4. Gene expression profile showed that PxdsRNase1 was mainly expressed in the hemolymph; and that PxdsRNase2 and PxdsRNase3 were mainly expressed in the intestinal tract. The expression of PxCht (Chitinase of P. xylostella) in P. xylostella larvae injected with the mixture of dsPxCht (dsRNA of PxCht) and dsPxdsRNase1 (dsRNA of PxdsRNase1), dsPxdsRNase2 (dsRNA of PxdsRNase2), or dsPxdsRNase3 (dsRNA of PxdsRNase3) was significantly higher than that in the larvae injected with the mixture of dsGFP (dsRNA of green fluorescent protein gene, GFP) and dsPxCht; the transcription level of PxCht in the larvae feeding on the mixture of dsPxCht and dsPxdsRNase1, dsPxdsRNase2, or dsPxdsRNase3 was significantly higher than that in the larvae feeding on the mixture of dsPxCht and dsGFP. The recombinant protein of PxdsRNase1 degraded dsRNA rapidly, PxdsRNase3 cleaved dsRNA without complete degradation, and PxdsRNase2 could not degrade dsRNA in vitro. These results suggested that PxdsRNases1, PxdsRNases2, and PxdsRNases3 were involved in the dsRNA degradation to reduce RNAi efficiency with different mechanisms.
英文关键词	dsRNase RNA interference diamondback moth RNA degradation
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000689175500001
WOS关键词	FUNCTIONAL-CHARACTERIZATION ; DSRNA DEGRADATION ; NUCLEASE ACTIVITY ; DESERT LOCUST ; PEA APHID ; INJECTION ; GUT ; EXPRESSION ; KNOCKDOWN ; EFFICACY
WOS类目	Entomology
WOS研究方向	Entomology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/363595
作者单位	[Chen, Jin-Zhi; Jiang, Ying-Xia; Li, Miao-Wen; Li, Jian-Wen; Zha, Ben-Hu; Yang, Guang] Fujian Agr & Forestry Univ, Inst Appl Ecol, State Key Lab Ecol Pest Control Fujian & Taiwan C, Fuzhou 350002, Peoples R China; [Chen, Jin-Zhi; Jiang, Ying-Xia; Li, Miao-Wen; Li, Jian-Wen; Zha, Ben-Hu; Yang, Guang] Minist Educ, Joint Int Res Lab Ecol Pest Control, Fuzhou 350002, Peoples R China; [Chen, Jin-Zhi; Jiang, Ying-Xia; Li, Miao-Wen; Li, Jian-Wen; Zha, Ben-Hu; Yang, Guang] Minist Agr & Rural Affairs, Key Lab Integrated Pest Management Fujian Taiwan, Fuzhou 350002, Peoples R China; [Chen, Jin-Zhi; Jiang, Ying-Xia; Li, Miao-Wen; Li, Jian-Wen; Zha, Ben-Hu; Yang, Guang] Fujian Prov Univ, Key Lab Green Control Insect Pests, Fujian Agr & Forestry Univ, Fuzhou 350002, Peoples R China; [Chen, Jin-Zhi; Jiang, Ying-Xia; Li, Miao-Wen; Li, Jian-Wen; Zha, Ben-Hu; Yang, Guang] Fujian Agr & Forestry Univ, Ministerial & Prov Joint Innovat Ctr Safety Prod, Fuzhou 350002, Peoples R China; [Yang, Guang] Fujian Agr & Forestry Univ, ...
推荐引用方式 GB/T 7714	Chen, Jin-Zhi,Jiang, Ying-Xia,Li, Miao-Wen,et al. Double-Stranded RNA-Degrading Enzymes Reduce the Efficiency of RNA Interference in Plutella xylostella[J],2021,12(8).
APA	Chen, Jin-Zhi,Jiang, Ying-Xia,Li, Miao-Wen,Li, Jian-Wen,Zha, Ben-Hu,&Yang, Guang.(2021).Double-Stranded RNA-Degrading Enzymes Reduce the Efficiency of RNA Interference in Plutella xylostella.INSECTS,12(8).
MLA	Chen, Jin-Zhi,et al."Double-Stranded RNA-Degrading Enzymes Reduce the Efficiency of RNA Interference in Plutella xylostella".INSECTS 12.8(2021).