干旱区生态环境知识资源中心(Arid): Wild Halophytic Phragmites karka Biomass Saccharification by Bacterial Enzyme Cocktail

Arid

DOI	10.3389/fmicb.2021.714940
	Wild Halophytic Phragmites karka Biomass Saccharification by Bacterial Enzyme Cocktail
	Ansari, Immad; Ejaz, Uroosa; Abideen, Zainul; Gulzar, Salman; Syed, Muhammad Noman; Liu, Jing; Li, Wang; Fu, Pengcheng; Sohail, Muhammad
通讯作者	Sohail, M (corresponding author), Univ Karachi, Dept Microbiol, Karachi, Pakistan. ; Sohail, M (corresponding author), Weihai UIC Biotechnol Inc, Weihai, Peoples R China.
来源期刊	FRONTIERS IN MICROBIOLOGY
EISSN	1664-302X
出版年	2021
卷号	12
英文摘要	Biofuel derived from halophytic biomass is getting attention owing to the concerns of energy versus food crisis. The disadvantages associated with edible bioenergy resources necessitate the need to explore new feedstocks for sustainable biofuel production. In this study, biomass from locally available abundant halophytes (Panicum antidotale, Phragmites karka, Halopyrum mucronatum, and Desmostachya bipinnata) was screened for saccharification by an enzyme cocktail composed of cellulase, xylanase, and pectinase from Brevibacillus borstelensis UE10 and UE27, Bacillus aestuarii UE25, Aneurinibacillus thermoaerophilus UE1, and Bacillus vallismortis MH 1. Two types of pretreatment, i.e., with dilute acid and freeze-thaw, were independently applied to the halophytic biomass. Saccharification of acid-pretreated P. karka biomass yielded maximum reducing sugars (9 mg g(-1)) as compared to other plants. Thus, the factors (temperature, pH, substrate concentration, and enzyme units) affecting its saccharification were optimized using central composite design. This statistical model predicted 49.8 mg g(-1) of reducing sugars that was comparable to the experimental value (40 mg g(-1)). Scanning electron microscopy and Fourier-transform infrared spectroscopy showed significant structural changes after pretreatment and saccharification. Therefore, halophytes growing in saline, arid, and semi-arid regions can be promising alternative sources for bioenergy production.
英文关键词	central composite design enzyme cocktail biomass Phragmites karka saccharification
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000703084200001
WOS关键词	SUGARCANE BAGASSE ; METHYLTRIOCTYLAMMONIUM CHLORIDE ; LIGNOCELLULOSIC BIOMASS ; ETHANOL-PRODUCTION ; CELLULASE ; OPTIMIZATION ; HYDROLYSIS ; PRETREATMENT ; CONVERSION ; AUSTRALIS
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/363325
作者单位	[Ansari, Immad; Ejaz, Uroosa; Sohail, Muhammad] Univ Karachi, Dept Microbiol, Karachi, Pakistan; [Ejaz, Uroosa] Shaheed Zulfikar Ali Bhutto Inst Sci & Technol, Dept Biosci, Karachi, Pakistan; [Abideen, Zainul; Gulzar, Salman] Univ Karachi, Dr Muhammad Ajmal Khan Inst Sustainable Halophyte, Karachi, Pakistan; [Syed, Muhammad Noman] Univ Karachi, Dept Biochem, Karachi, Pakistan; [Liu, Jing; Li, Wang; Fu, Pengcheng] Hainan Univ, State Key Lab Marine Resource Utilizat South Chin, Haikou, Hainan, Peoples R China; [Fu, Pengcheng; Sohail, Muhammad] Weihai UIC Biotechnol Inc, Weihai, Peoples R China
推荐引用方式 GB/T 7714	Ansari, Immad,Ejaz, Uroosa,Abideen, Zainul,et al. Wild Halophytic Phragmites karka Biomass Saccharification by Bacterial Enzyme Cocktail[J],2021,12.
APA	Ansari, Immad.,Ejaz, Uroosa.,Abideen, Zainul.,Gulzar, Salman.,Syed, Muhammad Noman.,...&Sohail, Muhammad.(2021).Wild Halophytic Phragmites karka Biomass Saccharification by Bacterial Enzyme Cocktail.FRONTIERS IN MICROBIOLOGY,12.
MLA	Ansari, Immad,et al."Wild Halophytic Phragmites karka Biomass Saccharification by Bacterial Enzyme Cocktail".FRONTIERS IN MICROBIOLOGY 12(2021).