干旱区生态环境知识资源中心(Arid): Mining for Perchlorate Resistance Genes in Microorganisms From Sediments of a Hypersaline Pond in Atacama Desert, Chile

Arid

DOI	10.3389/fmicb.2021.723874
	Mining for Perchlorate Resistance Genes in Microorganisms From Sediments of a Hypersaline Pond in Atacama Desert, Chile
	Diaz-Rullo, Jorge; Rodriguez-Valdecantos, Gustavo; Torres-Rojas, Felipe; Cid, Luis; Vargas, Ignacio T.; Gonzalez, Bernardo; Gonzalez-Pastor, Jose Eduardo
通讯作者	Gonzalez-Pastor, JE (corresponding author), INTA, CSIC, Ctr Astrobiol, Dept Mol Evolut, Madrid, Spain.
来源期刊	FRONTIERS IN MICROBIOLOGY
EISSN	1664-302X
出版年	2021
卷号	12
英文摘要	Perchlorate is an oxidative pollutant toxic to most of terrestrial life by promoting denaturation of macromolecules, oxidative stress, and DNA damage. However, several microorganisms, especially hyperhalophiles, are able to tolerate high levels of this compound. Furthermore, relatively high quantities of perchlorate salts were detected on the Martian surface, and due to its strong hygroscopicity and its ability to substantially decrease the freezing point of water, perchlorate is thought to increase the availability of liquid brine water in hyper-arid and cold environments, such as the Martian regolith. Therefore, perchlorate has been proposed as a compound worth studying to better understanding the habitability of the Martian surface. In the present work, to study the molecular mechanisms of perchlorate resistance, a functional metagenomic approach was used, and for that, a small-insert library was constructed with DNA isolated from microorganisms exposed to perchlorate in sediments of a hypersaline pond in the Atacama Desert, Chile (Salar de Maricunga), one of the regions with the highest levels of perchlorate on Earth. The metagenomic library was hosted in Escherichia coli DH10B strain and exposed to sodium perchlorate. This technique allowed the identification of nine perchlorate-resistant clones and their environmental DNA fragments were sequenced. A total of seventeen ORFs were predicted, individually cloned, and nine of them increased perchlorate resistance when expressed in E. coli DH10B cells. These genes encoded hypothetical conserved proteins of unknown functions and proteins similar to other not previously reported to be involved in perchlorate resistance that were related to different cellular processes such as RNA processing, tRNA modification, DNA protection and repair, metabolism, and protein degradation. Furthermore, these genes also conferred resistance to UV-radiation, 4-nitroquinoline-N-oxide (4-NQO) and/or hydrogen peroxide (H2O2), other stress conditions that induce oxidative stress, and damage in proteins and nucleic acids. Therefore, the novel genes identified will help us to better understand the molecular strategies of microorganisms to survive in the presence of perchlorate and may be used in Mars exploration for creating perchlorate-resistance strains interesting for developing Bioregenerative Life Support Systems (BLSS) based on in situ resource utilization (ISRU).
英文关键词	perchlorate-resistance oxidative stress tRNA modification DNA repair protein damage hypersaline environments Atacama Desert Mars
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000681631900001
WOS关键词	RECURRING SLOPE LINEAE ; DNA-BINDING PROTEIN ; ESCHERICHIA-COLI ; MESSENGER-RNA ; SYNTHETIC BIOLOGY ; OXIDATIVE STRESS ; SALT TOLERANCE ; SIGMA-FACTOR ; HU PROTEIN ; SP NOV.
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/363323
作者单位	[Diaz-Rullo, Jorge; Gonzalez-Pastor, Jose Eduardo] INTA, CSIC, Ctr Astrobiol, Dept Mol Evolut, Madrid, Spain; [Diaz-Rullo, Jorge] Univ Alcala De Henares, Polytech Sch, Alcala De Henares, Spain; [Rodriguez-Valdecantos, Gustavo; Cid, Luis; Gonzalez, Bernardo] Univ Adolfo Ibanez, Fac Sci & Engn, Santiago, Chile; [Rodriguez-Valdecantos, Gustavo; Cid, Luis; Gonzalez, Bernardo] Pontificia Univ Catolica Chile, Fac Biol Sci, Ctr Appl Ecol & Sustainabil CAPES, Santiago, Chile; [Torres-Rojas, Felipe; Vargas, Ignacio T.] Pontificia Univ Catolica Chile, Dept Hydraul & Environm Engn, Santiago, Chile; [Vargas, Ignacio T.] Ctr Desarrollo Urbano Sustentable CEDEUS, Santiago, Chile
推荐引用方式 GB/T 7714	Diaz-Rullo, Jorge,Rodriguez-Valdecantos, Gustavo,Torres-Rojas, Felipe,et al. Mining for Perchlorate Resistance Genes in Microorganisms From Sediments of a Hypersaline Pond in Atacama Desert, Chile[J],2021,12.
APA	Diaz-Rullo, Jorge.,Rodriguez-Valdecantos, Gustavo.,Torres-Rojas, Felipe.,Cid, Luis.,Vargas, Ignacio T..,...&Gonzalez-Pastor, Jose Eduardo.(2021).Mining for Perchlorate Resistance Genes in Microorganisms From Sediments of a Hypersaline Pond in Atacama Desert, Chile.FRONTIERS IN MICROBIOLOGY,12.
MLA	Diaz-Rullo, Jorge,et al."Mining for Perchlorate Resistance Genes in Microorganisms From Sediments of a Hypersaline Pond in Atacama Desert, Chile".FRONTIERS IN MICROBIOLOGY 12(2021).