干旱区生态环境知识资源中心(Arid): Description of Microbial Communities of Phosphate Mine Wastes in Morocco, a Semi-Arid Climate, Using High-Throughput Sequencing and Functional Prediction

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DOI	10.3389/fmicb.2021.666936
	Description of Microbial Communities of Phosphate Mine Wastes in Morocco, a Semi-Arid Climate, Using High-Throughput Sequencing and Functional Prediction
	Mghazli, Najoua; Sbabou, Laila; Hakkou, Rachid; Ouhammou, Ahmed; El Adnani, Mariam; Bruneel, Odile
通讯作者	Bruneel, O (corresponding author), Univ Montpellier, CNRS, IRD, HydroSci Montpellier, Montpellier, France.
来源期刊	FRONTIERS IN MICROBIOLOGY
EISSN	1664-302X
出版年	2021
卷号	12
英文摘要	Soil microbiota are vital for successful revegetation, as they play a critical role in nutrient cycles, soil functions, and plant growth and health. A rehabilitation scenario of the abandoned Kettara mine (Morocco) includes covering acidic tailings with alkaline phosphate mine wastes to limit water infiltration and hence acid mine drainage. Revegetation of phosphate wastes is the final step to this rehabilitation plan. However, revegetation is hard on this type of waste in semi-arid areas and only a few plants managed to grow naturally after 5 years on the store-and-release cover. As we know that belowground biodiversity is a key component for aboveground functioning, we sought to know if any structural problem in phosphate waste communities could explain the almost absence of plants. To test this hypothesis, bacterial and archaeal communities present in these wastes were assessed by 16S rRNA metabarcoding. Exploration of taxonomic composition revealed a quite diversified community assigned to 19 Bacterial and two Archaeal phyla, similar to other studies, that do not appear to raise any particular issues of structural problems. The dominant sequences belonged to Proteobacteria, Bacteroidetes, Actinobacteria, and Gemmatimonadetes and to the genera Massilia, Sphingomonas, and Adhaeribacter. LEfSe analysis identified 19 key genera, and metagenomic functional prediction revealed a broader phylogenetic range of taxa than expected, with all identified genera possessing at least one plant growth-promoting trait. Around 47% of the sequences were also related to genera possessing strains that facilitate plant development under biotic and environmental stress conditions, such as drought and heat.
英文关键词	phosphate mine wastes microbial communities PICRUSt prediction metabarcoding biodiversity
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000675559200001
WOS关键词	GROWTH-PROMOTING BACTERIA ; STRESS TOLERANCE ; SP NOV. ; SOIL ; DIVERSITY ; TAILINGS ; PLANTS ; DRAINAGE ; PHYTOREMEDIATION ; MICROORGANISMS
WOS类目	Microbiology
WOS研究方向	Microbiology
来源机构	French National Research Institute for Sustainable Development
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/363321
作者单位	[Mghazli, Najoua; Sbabou, Laila] Mohammed V Univ Rabat, Fac Sci, Team Microbiol & Mol Biol, Ctr Res Plants & Microbial Biotechnol Biodivers &, Rabat, Morocco; [Mghazli, Najoua; Bruneel, Odile] Univ Montpellier, CNRS, IRD, HydroSci Montpellier, Montpellier, France; [Hakkou, Rachid] Cadi Ayyad Univ UCA, Fac Sci & Technol, IMED Lab, Marrakech, Morocco; [Hakkou, Rachid] Mohammed VI Polytech Univ UM6P, Min Environm & Circular Econ Program, Benguerir, Morocco; [Ouhammou, Ahmed] Cadi Ayyad Univ, Fac Sci Semlalia, Lab Microbial Biotechnol Agrosci & Environm BioMA, Reg Herbarium MARK,Team Agrosci PhytoBiodivers &, Marrakech, Morocco; [El Adnani, Mariam] Mohammed V Univ Rabat, Natl Sch Mines Rabat, Resources Valorisat Environm & Sustainable Dev La, Rabat, Morocco
推荐引用方式 GB/T 7714	Mghazli, Najoua,Sbabou, Laila,Hakkou, Rachid,et al. Description of Microbial Communities of Phosphate Mine Wastes in Morocco, a Semi-Arid Climate, Using High-Throughput Sequencing and Functional Prediction[J]. French National Research Institute for Sustainable Development,2021,12.
APA	Mghazli, Najoua,Sbabou, Laila,Hakkou, Rachid,Ouhammou, Ahmed,El Adnani, Mariam,&Bruneel, Odile.(2021).Description of Microbial Communities of Phosphate Mine Wastes in Morocco, a Semi-Arid Climate, Using High-Throughput Sequencing and Functional Prediction.FRONTIERS IN MICROBIOLOGY,12.
MLA	Mghazli, Najoua,et al."Description of Microbial Communities of Phosphate Mine Wastes in Morocco, a Semi-Arid Climate, Using High-Throughput Sequencing and Functional Prediction".FRONTIERS IN MICROBIOLOGY 12(2021).