干旱区生态环境知识资源中心(Arid): Temporal and Spatial Changes in Phyllosphere Microbiome of Acacia Trees Growing in Arid Environments

Arid

DOI	10.3389/fmicb.2021.656269
	Temporal and Spatial Changes in Phyllosphere Microbiome of Acacia Trees Growing in Arid Environments
	Al Ashhab, Ashraf; Meshner, Shiri; Alexander-Shani, Rivka; Dimerets, Hana; Brandwein, Michael; Bar-Lavan, Yael; Winters, Gidon
通讯作者	Al Ashhab, A (corresponding author), Dead Sea & Arava Sci Ctr, Masada, Israel. ; Al Ashhab, A (corresponding author), Ben Gurion Univ Negev, Eilat Campus, Beer Sheva, Israel.
来源期刊	FRONTIERS IN MICROBIOLOGY
EISSN	1664-302X
出版年	2021
卷号	12
英文摘要	Background: The evolutionary relationships between plants and their microbiomes are of high importance to the survival of plants in general and even more in extreme conditions. Changes in the plant's microbiome can affect plant development, growth, fitness, and health. Along the arid Arava, southern Israel, acacia trees (Acacia raddiana and Acacia tortilis) are considered keystone species. In this study, we investigated the ecological effects of plant species, microclimate, phenology, and seasonality on the epiphytic and endophytic microbiome of acacia trees. One hundred thirty-nine leaf samples were collected throughout the sampling year and were assessed using 16S rDNA gene amplified with five different primers (targeting different gene regions) and sequenced (150 bp paired-end) on an Illumina MiSeq sequencing platform. Results: Epiphytic bacterial diversity indices (Shannon-Wiener, Chao1, Simpson, and observed number of operational taxonomic units) were found to be nearly double compared to endophyte counterparts. Epiphyte and endophyte communities were significantly different from each other in terms of the composition of the microbial associations. Interestingly, the epiphytic bacterial diversity was similar in the two acacia species, but the canopy sides and sample months exhibited different diversity, whereas the endophytic bacterial communities were different in the two acacia species but similar throughout the year. Abiotic factors, such as air temperature and precipitation, were shown to significantly affect both epiphyte and endophytes communities. Bacterial community compositions showed that Firmicutes dominate A. raddiana, and Proteobacteria dominate A. tortilis; these bacterial communities consisted of only a small number of bacterial families, mainly Bacillaceae and Comamonadaceae in the endophyte for A. raddiana and A. tortilis, respectively, and Geodematophilaceae and Micrococcaceae for epiphyte bacterial communities, respectively. Interestingly, similar to 60% of the obtained bacterial classifications were unclassified below family level (i.e., new ). Conclusions: These results shed light on the unique desert phyllosphere microbiome highlighting the importance of multiple genotypic and abiotic factors in shaping the epiphytic and endophytic microbial communities. This study also shows that only a few bacterial families dominate both epiphyte and endophyte communities, highlighting the importance of climate change (precipitation, air temperature, and humidity) in affecting arid land ecosystems where acacia trees are considered keystone species.
英文关键词	Acacia raddiana Acacia tortilis phyllosphere desert plants microbiome endophytes epiphytes
类型	Article
语种	英语
开放获取类型	Green Submitted, gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000678613800001
WOS关键词	BACTERIAL COMMUNITIES ; NEGEV DESERT ; LEAF SURFACE ; DIVERSITY ; PLANTS ; GROWTH ; LAIMOSPHERE ; SUCCESSION ; ADAPTATION ; MORTALITY
WOS类目	Microbiology
WOS研究方向	Microbiology
来源机构	Ben-Gurion University of the Negev ; Hebrew University of Jerusalem
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/363320
作者单位	[Al Ashhab, Ashraf; Meshner, Shiri; Alexander-Shani, Rivka; Dimerets, Hana; Brandwein, Michael; Bar-Lavan, Yael; Winters, Gidon] Dead Sea & Arava Sci Ctr, Masada, Israel; [Al Ashhab, Ashraf; Winters, Gidon] Ben Gurion Univ Negev, Eilat Campus, Beer Sheva, Israel; [Brandwein, Michael] Hebrew Univ Jerusalem, Fac Dent Med, Biofilm Res Lab, Jerusalem, Israel
推荐引用方式 GB/T 7714	Al Ashhab, Ashraf,Meshner, Shiri,Alexander-Shani, Rivka,et al. Temporal and Spatial Changes in Phyllosphere Microbiome of Acacia Trees Growing in Arid Environments[J]. Ben-Gurion University of the Negev, Hebrew University of Jerusalem,2021,12.
APA	Al Ashhab, Ashraf.,Meshner, Shiri.,Alexander-Shani, Rivka.,Dimerets, Hana.,Brandwein, Michael.,...&Winters, Gidon.(2021).Temporal and Spatial Changes in Phyllosphere Microbiome of Acacia Trees Growing in Arid Environments.FRONTIERS IN MICROBIOLOGY,12.
MLA	Al Ashhab, Ashraf,et al."Temporal and Spatial Changes in Phyllosphere Microbiome of Acacia Trees Growing in Arid Environments".FRONTIERS IN MICROBIOLOGY 12(2021).