干旱区生态环境知识资源中心(Arid): Novel Mouse Models of Fungal Asthma

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DOI	10.3389/fcimb.2021.683194
	Novel Mouse Models of Fungal Asthma
	Daines, Michael; Pereira, Rhea; Cunningham, Aubrey; Pryor, Barry; Besselsen, David G. G.; Liu, Yuchen; Luo, Qianwen; Chen, Yin
通讯作者	Chen, Y (corresponding author), Univ Arizona, Asthma & Airway Dis Res Ctr, Tucson, AZ 85721 USA. ; Chen, Y (corresponding author), Univ Arizona, Dept Pharmacol & Toxicol, Sch Pharm, Tucson, AZ 85721 USA.
来源期刊	FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
ISSN	2235-2988
出版年	2021
卷号	11
英文摘要	Alternaria alternata is a ubiquitous fungus and a major allergen associated with the development of asthma. Inhalation of intact spores is the primary cause of human exposure to fungal allergen. However, allergen-rich cultured fungal filtrates are oftentimes used in the current models of fungal sensitization that do not fully reflect real-life exposures. Thus, establishing novel spore exposure models is imperative. In this study, we established novel fungal exposure models of both adult and neonate to live spores. We examined pathophysiological changes in the spore models as compared to the non-exposure controls and also to the conventional filtrate models. While both Alternaria filtrate- and spore-exposed adult BALB/c mice developed elevated airway hyperresponsiveness (AHR), filtrates induced a greater IgE mediated response and higher broncholavage eosinophils than spores. In contrast, the mice exposed to Alternaria spores had higher numbers of neutrophils. Both exposures induced comparable levels of lung tissue inflammation and mucous cell metaplasia (MCM). In the neonatal model, exposure to Alternaria spores resulted in a significant increase of AHR in both adult and neonatal mice. Increased levels of IgE in both neonatal and adult mice exposed to spores was associated with increased eosinophilia in the treatment groups. Adult demonstrated increased numbers of lymphocytes that was paralleled by increased IgG1 production. Both adults and neonates demonstrated similarly increased eosinophilia, IgE, tissue inflammation and MCM.
英文关键词	Alternaria asthma AHR lung fungus pathophysiology
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000693617300001
WOS关键词	AIRWAY INFLAMMATION ; ALLERGIC INFLAMMATION ; EXPOSURE ; ALTERNARIA ; IMMUNITY ; DESERT ; MICE ; MOLD ; HYPERRESPONSIVENESS ; SENSITIZATION
WOS类目	Immunology ; Microbiology
WOS研究方向	Immunology ; Microbiology
来源机构	University of Arizona
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/363279
作者单位	[Daines, Michael; Pereira, Rhea; Cunningham, Aubrey] Univ Arizona, Coll Med, Dept Pediat, Tucson, AZ USA; [Daines, Michael; Chen, Yin] Univ Arizona, Asthma & Airway Dis Res Ctr, Tucson, AZ 85721 USA; [Pryor, Barry] Univ Arizona, Sch Plant Sci, Tucson, AZ USA; [Besselsen, David G. G.] Univ Arizona, Anim & Comparat Biomed Sci, Tucson, AZ USA; [Liu, Yuchen; Chen, Yin] Univ Arizona, Dept Pharmacol & Toxicol, Sch Pharm, Tucson, AZ 85721 USA; [Luo, Qianwen] Univ Arizona, Dept Biosyst Engn, Tucson, AZ USA
推荐引用方式 GB/T 7714	Daines, Michael,Pereira, Rhea,Cunningham, Aubrey,et al. Novel Mouse Models of Fungal Asthma[J]. University of Arizona,2021,11.
APA	Daines, Michael.,Pereira, Rhea.,Cunningham, Aubrey.,Pryor, Barry.,Besselsen, David G. G..,...&Chen, Yin.(2021).Novel Mouse Models of Fungal Asthma.FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,11.
MLA	Daines, Michael,et al."Novel Mouse Models of Fungal Asthma".FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY 11(2021).