干旱区生态环境知识资源中心(Arid): Determination of antidepressants and benzodiazepines in paired hair and nail samples

Arid

DOI	10.1016/j.forsciint.2021.110935
	Determination of antidepressants and benzodiazepines in paired hair and nail samples
	Cobo-Golpe, Maria; De-Castro-Rios, Ana; Cruz, Angelines; Paramo, Mario; Lopez-Rivadulla, Manuel; Lendoiro, Elena
通讯作者	Lendoiro, E (corresponding author), Univ Santiago de Compostela, Serv Toxicol, Inst Ciencias Forenses, San Francisco S-N, Santiago De Compostela 15782, Spain.
来源期刊	FORENSIC SCIENCE INTERNATIONAL
ISSN	0379-0738
EISSN	1872-6283
出版年	2021
卷号	326
英文摘要	Hair and nails are keratinized matrices that can be used in Toxicology as matrices for the long-term detection of substances. Whereas hair is an established matrix with decades of use in this field, nails have been less studied, especially including a comparison to hair samples. Specifically in the case of antidepressant and benzodiazepine drugs, very few publications analyzing these drugs in nail samples exist as of yet. For this reason, in the present study a method for the detection of 12 antidepressant and benzodiazepine drugs in hair and nail samples was developed. Samples were decontaminated with 3 washes of dichloromethane, and 25 or 30 mg of hair and nails, respectively, were pulverized. Then, the samples were incubated with 1.5 mL water:ACN (50:50, v/v) with horizontal agitation for 90 min. The supernatant was evaporated and reconstituted in 200 mu L of methanol and 2 mL of 2% FA in water, submitted to solid phase extraction (SPE) using Oasis MCX cartridges and analyzed by LC-MS/MS. The method was satisfactorily validated in nail and hair samples for the following parameters: linearity, LOD (0.0 05-0.02 ng/mg), LOQ (0.01-0.02 ng/mg), selectivity, carryover, accuracy, imprecision, matrix effect, extraction efficiency, process efficiency and autosampler stability. Matched fingernail, toenail and hair samples were obtained from 21 patients under treatment with any of the studied drugs and analyzed with the developed method. The most frequently detected drugs were venlafaxine (n = 11), trazodone (n = 6), zolpidem (n = 5), alprazolam (n = 5) and nordiazepam (n = 5). Concentrations in hair, fingernails and toenails, respectively, were 44.31 ng/mg, 8.05-43.35 ng/mg and 7.02-22.69 ng/mg for venlafaxine; 5.40-19.08 ng/mg, 0.13-1.00 ng/mg and 0.42-1.04 ng/mg for trazodone; 13.86 ng/mg, 5.19 ng/mg and 9.11 ng/mg for fluoxetine; 7.42 ng/mg, 1.85 ng/ mg and 0.03-2.81 ng/mg for sertraline; 0.40-1.42 ng/mg, 0.12 ng/mg and 0.16 ng/mg for zolpidem; and 0.02-0.11 ng/mg, 0.07-1.07 ng/mg and 0.05 ng/mg for alprazolam for the patients under active treatment. Hair concentrations were higher than nail concentrations for most drugs in patients under active treatment, with the exception of diazepam (n = 1; 0.12 ng/mg in hair and 0.41 ng/mg in fingernails). Fingernail concentrations were lower than toenail concentrations in patients under active treatment in most compared cases. Comparison of fingernails and toenails of a patient with antifungal treatment did not show an observable effect in concentrations. (c) 2021 The Author(s). Published by Elsevier B.V. CC_BY_4.0
英文关键词	Antidepressant Benzodiazepine Hair Nail LC-MS MS
类型	Article
语种	英语
开放获取类型	hybrid
收录类别	SCI-E
WOS记录号	WOS:000687303000011
WOS关键词	INDIVIDUAL DOSAGE HISTORY ; HUMAN SCALP HAIR ; ETHYL GLUCURONIDE ; FINGERNAIL CLIPPINGS ; ALTERNATIVE MATRIX ; COCAINE ANALYTES ; WORKPLACE DRUG ; EXPOSURE ; OPIATES ; METHAMPHETAMINE
WOS类目	Medicine, Legal
WOS研究方向	Legal Medicine
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/363251
作者单位	[Cobo-Golpe, Maria; De-Castro-Rios, Ana; Cruz, Angelines; Lopez-Rivadulla, Manuel; Lendoiro, Elena] Univ Santiago de Compostela, Serv Toxicol, Inst Ciencias Forenses, San Francisco S-N, Santiago De Compostela 15782, Spain; [Paramo, Mario] Complexo Hosp Univ Santiago CHUS, Serv Galego Saude SERGAS, Serv Psiquiatria, Santiago De Compostela, Spain
推荐引用方式 GB/T 7714	Cobo-Golpe, Maria,De-Castro-Rios, Ana,Cruz, Angelines,et al. Determination of antidepressants and benzodiazepines in paired hair and nail samples[J],2021,326.
APA	Cobo-Golpe, Maria,De-Castro-Rios, Ana,Cruz, Angelines,Paramo, Mario,Lopez-Rivadulla, Manuel,&Lendoiro, Elena.(2021).Determination of antidepressants and benzodiazepines in paired hair and nail samples.FORENSIC SCIENCE INTERNATIONAL,326.
MLA	Cobo-Golpe, Maria,et al."Determination of antidepressants and benzodiazepines in paired hair and nail samples".FORENSIC SCIENCE INTERNATIONAL 326(2021).