干旱区生态环境知识资源中心(Arid): Bioactivities of endophytic actinobacteria inhabiting Artemisia herba-alba emphasizing differences from free-living strains

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DOI	10.1007/s12223-021-00911-y
	Bioactivities of endophytic actinobacteria inhabiting Artemisia herba-alba emphasizing differences from free-living strains
	El-Shatoury, Sahar A.; Mahmoud, Fatma M.; El-Kazzaz, Waleed M.
通讯作者	El-Shatoury, SA (corresponding author), Suez Canal Univ, Fac Sci, Bot & Microbiol Dept, Ismailia 41522, Egypt.
来源期刊	FOLIA MICROBIOLOGICA
ISSN	0015-5632
EISSN	1874-9356
出版年	2021-09
英文摘要	The endophytic actinobacteria associated with Artemisia herba-alba (synonym: Seriphidium herba-alba) are highly diverse. This study aimed to illustrate the extent of their differences from the free-living actinobacteria in the surrounding environment. A selection of eighteen actinobacteria inhabiting A. herba-alba were compared with twenty and ten actinobatceria isolates from the surrounding desert and groundwater, respectively, representing six genera. Antagonistic and enzymatic activities, plant growth-promoting traits, and the occurrence of biosynthetic genes were compared among the isolates. Data were analyzed statistically using principal component analysis (PCA) and were visualized using heat map. Endophytic strains showed higher antimicrobial activity and production of plant growth promoters compared to desert and groundwater strains. Polyketide synthase and non-ribosomal peptide synthetase gene clusters were detected at higher frequencies in the endophytic strains (8 and 11 strains, respectively) than the desert strains (1 and 2 strains, respectively). In contrast, both gene clusters were not detected in the groundwater strains. The PCA revealed unique metabolic characteristics of the endophytes. The heatmap clustered the endophytic strains apart from the free-living strains, indicating distinctive qualitative and quantitative bioactivities. Analysis of 16S rRNA genes confirmed the chemotaxonomic identity of all but two strains, with > 94.5% similarity. Six endophytes displayed < 99.5% similarity with their closest type strains, which might indicate species novelty. This study provides an evidence of functional differences and possible species novelty of the endophytic actinobacteria inhabiting A. herba-alba, compared with the free-living species.
类型	Article ; Early Access
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000696424100001
WOS关键词	GROWTH-PROMOTING TRAITS ; GENE-SEQUENCES ; DIVERSITY ; BACTERIA ; MICROMONOSPORA ; PLANTS ; L.
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/363243
作者单位	[El-Shatoury, Sahar A.; Mahmoud, Fatma M.; El-Kazzaz, Waleed M.] Suez Canal Univ, Fac Sci, Bot & Microbiol Dept, Ismailia 41522, Egypt
推荐引用方式 GB/T 7714	El-Shatoury, Sahar A.,Mahmoud, Fatma M.,El-Kazzaz, Waleed M.. Bioactivities of endophytic actinobacteria inhabiting Artemisia herba-alba emphasizing differences from free-living strains[J],2021.
APA	El-Shatoury, Sahar A.,Mahmoud, Fatma M.,&El-Kazzaz, Waleed M..(2021).Bioactivities of endophytic actinobacteria inhabiting Artemisia herba-alba emphasizing differences from free-living strains.FOLIA MICROBIOLOGICA.
MLA	El-Shatoury, Sahar A.,et al."Bioactivities of endophytic actinobacteria inhabiting Artemisia herba-alba emphasizing differences from free-living strains".FOLIA MICROBIOLOGICA (2021).