干旱区生态环境知识资源中心(Arid): Persistence and spread of tetracycline resistance genes and microbial community variations in the soil of animal corrals in a semi-arid planted forest

Arid

DOI	10.1093/femsec/fiab106
	Persistence and spread of tetracycline resistance genes and microbial community variations in the soil of animal corrals in a semi-arid planted forest
	Nejidat, Ali; Diaz-Reck, Damiana; Gelfand, Ilya; Zaady, Eli
通讯作者	Nejidat, A (corresponding author), Ben Gurion Univ Negev, Jacob Blaustein Inst Desert Res, Zuckerberg Inst Water Res, Dept Environm Hydrol & Microbiol, Sede Boger Campus, IL-84990 Midreshet Ben Gurion, Israel.
来源期刊	FEMS MICROBIOLOGY ECOLOGY
ISSN	0168-6496
EISSN	1574-6941
出版年	2021
卷号	97 期号:8
英文摘要	At the spring, goat and sheep herds are transferred to planted forests, in a semi-arid region in the northern Negev Desert, Israel, to reduce herbaceous biomass and, fire risk. The herds are held overnight in corrals for about 4 months, enriching the soil with organic matter and nitrogen. This research examined the effect of these enrichments on soil bacterial community structure (BCS) and the abundance of tetracycline resistance genes (TRGs) in active and abandoned corrals (1-10-years-old). Based on 16S rRNA gene sequences, the Proteobacteria and Actinobacteria phyla dominated the soil of all corrals. The Actinobacteria were less abundant in the active and 1-year-old corrals (23-26%) than in the other corrals and the control (33-38%). A principal component analysis showed that, the BCS in the active and the 1-year-old abandoned corrals was significantly different from that in the older corrals and the control. The Firmicutes phylum constituted 28% of the BCS in the active corrals, 12.5% in the 1-year-old corrals and 2% in the older corrals and the control. In contrast, the Acidobacteria phylum was hardly detected in the active and 1-year-old abandoned corrals and constituted 10% of the BCS in the older corrals. Genes conferring resistance to tetracycline were detected in high numbers. The tetG and tetW genes were detected in the active and abandoned corrals (1-10 years). The tetQ gene was detected only in the active and 1-year-old abandoned corrals. None of the genes were detected in the control soil. The three genes were detected outside an active corral, in the downstream section of an ephemeral tributary. The results prove that abandoned and unobserved periodic animal corrals are an environmental reservoir for TRGs.
英文关键词	night herd corrals microbial community tetracycline resistance genes nitrous oxide emissions
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000697725900017
WOS关键词	AMMONIA-OXIDIZING ARCHAEA ; WASTE-WATER IRRIGATION ; ANTIBIOTIC-RESISTANCE ; DENITRIFYING BACTERIA ; ARABLE SOIL ; SP NOV. ; MANURE ; DIVERSITY ; ABUNDANCE ; IMPACT
WOS类目	Microbiology
WOS研究方向	Microbiology
来源机构	Ben-Gurion University of the Negev
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/363230
作者单位	[Nejidat, Ali; Diaz-Reck, Damiana] Ben Gurion Univ Negev, Jacob Blaustein Inst Desert Res, Zuckerberg Inst Water Res, Dept Environm Hydrol & Microbiol, Sede Boger Campus, IL-84990 Midreshet Ben Gurion, Israel; [Gelfand, Ilya] Ben Gurion Univ Negev, Jacob Blaustein Inst Desert Res, French Associates Inst Agr & Biotechnol Drylands, Sede Boger Campus, IL-84990 Midreshet Ben Gurion, Israel; [Zaady, Eli] Agr Res Org, Dept Nat Resources, Gilat Res Ctr, Post Negev, IL-8531100 Mobile, Israel
推荐引用方式 GB/T 7714	Nejidat, Ali,Diaz-Reck, Damiana,Gelfand, Ilya,et al. Persistence and spread of tetracycline resistance genes and microbial community variations in the soil of animal corrals in a semi-arid planted forest[J]. Ben-Gurion University of the Negev,2021,97(8).
APA	Nejidat, Ali,Diaz-Reck, Damiana,Gelfand, Ilya,&Zaady, Eli.(2021).Persistence and spread of tetracycline resistance genes and microbial community variations in the soil of animal corrals in a semi-arid planted forest.FEMS MICROBIOLOGY ECOLOGY,97(8).
MLA	Nejidat, Ali,et al."Persistence and spread of tetracycline resistance genes and microbial community variations in the soil of animal corrals in a semi-arid planted forest".FEMS MICROBIOLOGY ECOLOGY 97.8(2021).