干旱区生态环境知识资源中心(Arid): Alphaproteobacteria facilitate Trichodesmium community trimethylamine utilization

Arid

DOI	10.1111/1462-2920.15773
	Alphaproteobacteria facilitate Trichodesmium community trimethylamine utilization
	Conover, Asa E.; Morando, Michael; Zhao, Yiming; Semones, Jacob; Hutchins, David A.; Webb, Eric A.
通讯作者	Webb, EA (corresponding author), Univ Southern Calif, Dept Marine & Environm Biol, Los Angeles, CA 90007 USA.
来源期刊	ENVIRONMENTAL MICROBIOLOGY
ISSN	1462-2912
EISSN	1462-2920
出版年	2021
英文摘要	In the surface waters of the warm oligotrophic ocean, filaments and aggregated colonies of the nitrogen (N)-fixing cyanobacterium Trichodesmium create microscale nutrient-rich oases. These hotspots fuel primary productivity and harbour a diverse consortium of heterotrophs. Interactions with associated microbiota can affect the physiology of Trichodesmium, often in ways that have been predicted to support its growth. Recently, it was found that trimethylamine (TMA), a globally abundant organic N compound, inhibits N-2 fixation in cultures of Trichodesmium without impairing growth rate, suggesting that Trichodesmium can use TMA as an alternate N source. In this study, N-15-TMA DNA stable isotope probing (SIP) of a Trichodesmium enrichment was employed to further investigate TMA metabolism and determine whether TMA-N is incorporated directly or secondarily via cross-feeding facilitated by microbial associates. Herein, we identify two members of the marine Roseobacter clade (MRC) of Alphaproteobacteria as the likely metabolizers of TMA and provide genomic evidence that they converted TMA into a more readily available form of N, e.g., ammonium (NH4+), which was subsequently used by Trichodesmium and the rest of the community. The results implicate microbiome-mediated carbon (C) and N transformations in modulating N-2 fixation and thus highlight the involvement of host-associated heterotrophs in global biogeochemical cycling.
类型	Article
语种	英语
开放获取类型	Green Submitted
收录类别	SCI-E
WOS记录号	WOS:000707128700001
WOS关键词	NITROGEN-FIXATION ; N-OXIDE ; MOLECULAR ASSESSMENT ; COMPATIBLE SOLUTE ; GLYCINE BETAINE ; MARINE ; METABOLISM ; BACTERIA ; METHYLAMINES ; IRON
WOS类目	Microbiology
WOS研究方向	Microbiology
来源机构	University of California, Berkeley
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/363124
作者单位	[Conover, Asa E.; Morando, Michael; Zhao, Yiming; Semones, Jacob; Hutchins, David A.; Webb, Eric A.] Univ Southern Calif, Dept Marine & Environm Biol, Los Angeles, CA 90007 USA; [Conover, Asa E.] Univ Calif Berkeley, Dept Integrat Biol, Berkeley, CA 94720 USA; [Morando, Michael] Univ Calif Santa Cruz, Ocean Sci Dept, Santa Cruz, CA 95064 USA
推荐引用方式 GB/T 7714	Conover, Asa E.,Morando, Michael,Zhao, Yiming,et al. Alphaproteobacteria facilitate Trichodesmium community trimethylamine utilization[J]. University of California, Berkeley,2021.
APA	Conover, Asa E.,Morando, Michael,Zhao, Yiming,Semones, Jacob,Hutchins, David A.,&Webb, Eric A..(2021).Alphaproteobacteria facilitate Trichodesmium community trimethylamine utilization.ENVIRONMENTAL MICROBIOLOGY.
MLA	Conover, Asa E.,et al."Alphaproteobacteria facilitate Trichodesmium community trimethylamine utilization".ENVIRONMENTAL MICROBIOLOGY (2021).