干旱区生态环境知识资源中心(Arid): New types of ATP-grasp ligase are associated with the novel pathway for complicated mycosporine-like amino acid production in desiccation-tolerant cyanobacteria

Arid

DOI	10.1111/1462-2920.15732
	New types of ATP-grasp ligase are associated with the novel pathway for complicated mycosporine-like amino acid production in desiccation-tolerant cyanobacteria
	Zhang, Zhong-Chun; Wang, Kai; Hao, Fu-Hua; Shang, Jin-Long; Tang, Hui-Ru; Qiu, Bao-Sheng
通讯作者	Qiu, BS (corresponding author), Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China. ; Qiu, BS (corresponding author), Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China. ; Tang, HR (corresponding author), Fudan Univ, State Key Lab Genet Engn, Sch Life Sci, Shanghai 200438, Peoples R China. ; Tang, HR (corresponding author), Fudan Univ, Zhongshan Hosp, Collaborat Innovat Ctr Genet & Dev, Minist Educ,Key Lab Contemporary Anthropol,Metabo, Shanghai 200438, Peoples R China.
来源期刊	ENVIRONMENTAL MICROBIOLOGY
ISSN	1462-2912
EISSN	1462-2920
出版年	2021-08
英文摘要	Mycosporine-like amino acids (MAAs) were widespread in diverse organisms to attenuate UV radiation. We recently characterized the large, complicated MAA mycosporine-2-(4-deoxygadusolyl-ornithine) in desert cyanobacterium Nostoc flagelliforme. Synthesis of this MAA requires the five-gene cluster mysABDC2C3. Here, bioinformatic analysis indicated that mysC duplication within five-gene mys clusters is strictly limited to drought-tolerant cyanobacteria. Phylogenic analysis distinguished these duplicated MysCs into two clades that separated from canonical MysCs. Heterologous expression of N. flagelliforme mys genes in Escherichia coli showed that MysAB produces 4-deoxygadusol. The ATP-grasp ligase of MysC3 catalyses the linkage of the delta- or epsilon-amino group of ornithine/lysine to 4-deoxygadusol, yielding mycosporine-ornithine or mycosporine-lysine respectively. The ATP-grasp ligase of MysC2 strictly condenses the alpha-amino group of mycosporine-ornithine to another 4-deoxygadusol. MysD (D-Ala-D-Ala ligase) functions following MysC2 to catalyse the formation of mycosporine-2-(4-deoxygadusolyl-ornithine). High arginine content likely provides a greater pool of ornithine over other amino acids during rehydration of desiccated N. flagelliforme. Duplication of ATP-grasp ligases is specific for the use of substrates that have two amino groups (such as ornithine) for the production of complicated MAAs with multiple chromophores. This five-enzyme biosynthesis pathway for complicated MAAs is a novel adaptation of cyanobacteria for UV tolerance in drought environments.
类型	Article ; Early Access
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000690784900001
WOS关键词	BIOSYNTHESIS ; IDENTIFICATION ; ACCUMULATION ; SHINORINE ; DISCOVERY ; RADIATION ; ORNITHINE ; STRAINS ; GENE
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/363122
作者单位	[Zhang, Zhong-Chun; Wang, Kai; Shang, Jin-Long; Qiu, Bao-Sheng] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China; [Zhang, Zhong-Chun; Wang, Kai; Shang, Jin-Long; Qiu, Bao-Sheng] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China; [Hao, Fu-Hua] Chinese Acad Sci, Wuhan Ctr Magnet Resonance, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Hubei, Peoples R China; [Tang, Hui-Ru] Fudan Univ, State Key Lab Genet Engn, Sch Life Sci, Shanghai 200438, Peoples R China; [Tang, Hui-Ru] Fudan Univ, Zhongshan Hosp, Collaborat Innovat Ctr Genet & Dev, Minist Educ,Key Lab Contemporary Anthropol,Metabo, Shanghai 200438, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Zhong-Chun,Wang, Kai,Hao, Fu-Hua,et al. New types of ATP-grasp ligase are associated with the novel pathway for complicated mycosporine-like amino acid production in desiccation-tolerant cyanobacteria[J],2021.
APA	Zhang, Zhong-Chun,Wang, Kai,Hao, Fu-Hua,Shang, Jin-Long,Tang, Hui-Ru,&Qiu, Bao-Sheng.(2021).New types of ATP-grasp ligase are associated with the novel pathway for complicated mycosporine-like amino acid production in desiccation-tolerant cyanobacteria.ENVIRONMENTAL MICROBIOLOGY.
MLA	Zhang, Zhong-Chun,et al."New types of ATP-grasp ligase are associated with the novel pathway for complicated mycosporine-like amino acid production in desiccation-tolerant cyanobacteria".ENVIRONMENTAL MICROBIOLOGY (2021).