干旱区生态环境知识资源中心(Arid): Comparison of different chiral selectors for the enantiomeric determination of amphetamine-type substances in human urine by solid-phase extraction followed by capillary electrophoresis-tandem mass spectrometry

Arid

DOI	10.1002/elps.202100231
	Comparison of different chiral selectors for the enantiomeric determination of amphetamine-type substances in human urine by solid-phase extraction followed by capillary electrophoresis-tandem mass spectrometry
	Pascual-Caro, Sergi; Borrull, Francesc; Aguilar, Carme; Calull, Marta
通讯作者	Aguilar, C (corresponding author), Univ Rovira & Virgili, Dept Analyt Chem & Organ Chem, Campus Sescelades,Marcelli Domingo 1, Tarragona 43007, Spain.
来源期刊	ELECTROPHORESIS
ISSN	0173-0835
EISSN	1522-2683
出版年	2022
英文摘要	The present study develops a method for the enantioseparation of a group of amphetamines and their metabolites in urine by CE coupled to MS/MS (CE-MS/MS). Amphetamines present a chiral center and thus two enantiomers, which is important from a toxicological point of view because they may have different pharmacokinetic and pharmacological properties. It is therefore essential to find suitable methods to distinguish both enantiomers. Today the use of CE is becoming more important in this field since, with the simple addition of a chiral selector to the background electrolyte, the enantioseparation can easily be achieved. However, when CE is coupled to MS, the use of volatile chiral selectors and compatible background electrolytes or other strategies such as the countercurrent migration approach are required to avoid contamination of the ion source from nonvolatile species. In the present study, we use the latter strategy to evaluate six different chiral selectors using CE-MS/MS. As a sample pre-treatment, two cationic-exchange sorbents-Oasis WCX and Oasis MCX-are compared for the urine pre-treatment. Using this method, it was possible to achieve the complete chiral separation of the amphetamines under study with detection limits ranging between 0.8 and 1.5 ng/mL and method quantification limits between 2.0 and 8.0 ng/mL. Matrix-matched calibration curves up to 150 ng/mL were used to cover the usual concentration ranges at which amphetamines have generally been found in toxicological and forensic analyses.
英文关键词	Amphetamine-type substances Cation-exchange sorbents CE-MS MS Chiral selectors Human urine
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000710336700001
WOS关键词	LIQUID-CHROMATOGRAPHY ; METHAMPHETAMINE ; METABOLITES ; SEPARATION ; LISDEXAMFETAMINE ; DERIVATIZATION ; DERIVATIVES ; STIMULANTS ; HAIR
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/363061
作者单位	[Pascual-Caro, Sergi; Borrull, Francesc; Aguilar, Carme; Calull, Marta] Univ Rovira & Virgili, Dept Analyt Chem & Organ Chem, Campus Sescelades,Marcelli Domingo 1, Tarragona 43007, Spain
推荐引用方式 GB/T 7714	Pascual-Caro, Sergi,Borrull, Francesc,Aguilar, Carme,et al. Comparison of different chiral selectors for the enantiomeric determination of amphetamine-type substances in human urine by solid-phase extraction followed by capillary electrophoresis-tandem mass spectrometry[J],2022.
APA	Pascual-Caro, Sergi,Borrull, Francesc,Aguilar, Carme,&Calull, Marta.(2022).Comparison of different chiral selectors for the enantiomeric determination of amphetamine-type substances in human urine by solid-phase extraction followed by capillary electrophoresis-tandem mass spectrometry.ELECTROPHORESIS.
MLA	Pascual-Caro, Sergi,et al."Comparison of different chiral selectors for the enantiomeric determination of amphetamine-type substances in human urine by solid-phase extraction followed by capillary electrophoresis-tandem mass spectrometry".ELECTROPHORESIS (2022).