干旱区生态环境知识资源中心(Arid): RNA-seq analysis of gene expression changes in cuticles during the larval-pupal metamorphosis of Plutella xylostella

Arid

DOI	10.1016/j.cbd.2021.100869
	RNA-seq analysis of gene expression changes in cuticles during the larval-pupal metamorphosis of Plutella xylostella
	Hou, Qiu-Li; Chen, Er-Hu
通讯作者	Chen, EH (corresponding author), Yangzhou Univ, Coll Hort & Plant Protect, Yangzhou 225009, Jiangsu, Peoples R China.
来源期刊	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
ISSN	1744-117X
EISSN	1878-0407
出版年	2021
卷号	39
英文摘要	The diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae) is a holometabolous insect that its cuticles must undergo the significant changes during the larval-pupal metamorphosis development. To elucidate these changes at molecular levels, RNA-seq analysis of cuticles from LLS (later fourth instar larval stage), PPS (pre-pupal stage) and PS (pupal stage) were performed in P. xylostella. In this paper, a total of 17,710 transcripts were obtained in the larval-pupal transition of P. xylostella, and out of which 2293 (881 up-regulated and 1412 downregulated) and 2989 transcripts (2062 up-regulated and 927 down-regulated) were identified to be differentially expressed between LLS and PPS, as well as PPS and PS, respectively. The further GO and KEGG analysis of differentially expressed genes (DEGs) revealed that the 'structural constituent of cuticle', 'chitin metabolic process', 'chitin binding', 'tyrosine metabolism' and 'insect hormone biosynthesis' pathways were significantly enriched, indicating these pathways might be involved in the process of larval pupation in P. xylostella. Then, we found some genes that encoded cuticular proteins, chitinolytic enzymes, chitin synthesis enzymes, and cuticle tanning proteins changed their expression levels remarkably, indicating these genes might play important roles in the restruction (degradation and biosynthesis) of insect cuticles during the larval metamorphosis. Additionally, the significant changes in the mRNA levels of 20-hydroxyecdysone (20E) and juvenile hormone (JH) related genes suggested their crucial roles in regulating cuticle remodeling during the larval metamorphosis of P. xylostella. In conclusion, the present study provide us the comprehensive gene expression profiles to explore the molecular mechanisms of cuticle metamorphosis in P. xylostella, which laid a molecular basis to study roles of specific pathways and genes in insect development.
英文关键词	Plutella xylostella Transcriptome RNA-Seq Larval pupation Cuticle metamorphosis
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000686901600001
WOS关键词	CUTICULAR PROTEIN GENES ; RED FLOUR BEETLE ; GENOME-WIDE IDENTIFICATION ; ORIENTAL FRUIT-FLY ; JUVENILE-HORMONE ; DIAMONDBACK MOTH ; DEVELOPMENTAL EXPRESSION ; CHITIN METABOLISM ; BROWN PLANTHOPPER ; DESERT LOCUST
WOS类目	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS研究方向	Biochemistry & Molecular Biology ; Genetics & Heredity
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/362892
作者单位	[Hou, Qiu-Li] Nanjing Univ Finance & Econ, Key Lab Grains & Oils Qual Control & Proc, Collaborat Innovat Ctr Modern Grain Circulat & Sa, Coll Food Sci & Engn, Nanjing 210023, Peoples R China; [Chen, Er-Hu] Yangzhou Univ, Coll Hort & Plant Protect, Yangzhou 225009, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Hou, Qiu-Li,Chen, Er-Hu. RNA-seq analysis of gene expression changes in cuticles during the larval-pupal metamorphosis of Plutella xylostella[J],2021,39.
APA	Hou, Qiu-Li,&Chen, Er-Hu.(2021).RNA-seq analysis of gene expression changes in cuticles during the larval-pupal metamorphosis of Plutella xylostella.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,39.
MLA	Hou, Qiu-Li,et al."RNA-seq analysis of gene expression changes in cuticles during the larval-pupal metamorphosis of Plutella xylostella".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 39(2021).