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DOI	10.1016/j.chemosphere.2021.132300
	Application of 96-well plate SPE method for analysis of persistent organic pollutants in low volume blood serum samples
	Palat, Jiri; Kukucka, Petr; Codling, Garry P.; Price, Elliott J.; Janku, Petr; Klanova, Jana
通讯作者	Kukucka, P (corresponding author), Masaryk Univ, RECETOX Ctr, Kamenice 753-5, Brno 62500, Czech Republic.
来源期刊	CHEMOSPHERE
ISSN	0045-6535
EISSN	1879-1298
出版年	2022
卷号	287
英文摘要	Though many persistent organic pollutants (POPs) are closely regulated the human population is still exposed to these ubiquitous chemicals from the environment and diet. Safe management and human biomonitoring of POPs is necessary to understand the risk of exposure. Within human biomonitoring the mass of sample is often limited, therefore robust methods using smaller sample amounts are necessary. This study developed a 96-well plate solid phase extraction (SPE) method for determination of selected POPs: polychlorinated biphenyls (PCBs), organo-chlorine pesticides (OCPs), polybrominated diphenyl ethers (PBDEs), hexabromocyclododecane (HBCD) and non-persistent novel flame retardants (NFRs) in low volume blood serum. Non-destructive clean-up coupling Oasis HLB extraction plate with Phree phospholipid removal plate was employed. Extraction efficiency was determined at low and high concentrations in certified reference materials NIST SRM 1957 and 1958, respec-tively. Target compounds deviated from certified values on average by 15% and 21% for SRM 1957 and SRM 1958, respectively. Observed limit of detections (LODs) ranged from 0.36 pg/mL (PCB 180) to 66.07 pg/mL (delta-HCH). The applicability for real samples is demonstrated on 48 samples from pregnant women enrolled in the pilot phase of the CELSPAC: TNG study. In total, 30 target compounds were detected in at least one sample. The method developed here provides a fast and reliable analysis of human blood serum with possibility to introduce automation for the sample preparation procedure.
英文关键词	POPs NFRs Blood serum Well-plate SPE Chromatography-mass spectrometry
类型	Article
语种	英语
开放获取类型	hybrid
收录类别	SCI-E
WOS记录号	WOS:000704944900007
WOS关键词	BROMINATED FLAME RETARDANTS ; NUTRITION EXAMINATION SURVEY ; DIPHENYL ETHERS PBDES ; NATIONAL-HEALTH ; HUMAN EXPOSURE ; POPS ; ASSOCIATION ; PREVALENCE ; PLASMA
WOS类目	Environmental Sciences
WOS研究方向	Environmental Sciences & Ecology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/362851
作者单位	[Palat, Jiri; Kukucka, Petr; Codling, Garry P.; Price, Elliott J.; Klanova, Jana] Masaryk Univ, RECETOX Ctr, Kamenice 753-5, Brno 62500, Czech Republic; [Codling, Garry P.] Univ Saskatchewan, Toxicol Ctr, 44 Campus Dr, Saskatoon, SK S7N 5B3, Canada; [Janku, Petr] Masaryk Univ Brno, Univ Hosp Brno, Dept Gynecol & Obstet, Brno, Czech Republic; [Janku, Petr] Masaryk Univ Brno, Fac Med, Brno, Czech Republic; [Janku, Petr] Masaryk Univ Brno, Fac Med, Dept Nursing & Midwifery, Brno, Czech Republic
推荐引用方式 GB/T 7714	Palat, Jiri,Kukucka, Petr,Codling, Garry P.,et al. Application of 96-well plate SPE method for analysis of persistent organic pollutants in low volume blood serum samples[J],2022,287.
APA	Palat, Jiri,Kukucka, Petr,Codling, Garry P.,Price, Elliott J.,Janku, Petr,&Klanova, Jana.(2022).Application of 96-well plate SPE method for analysis of persistent organic pollutants in low volume blood serum samples.CHEMOSPHERE,287.
MLA	Palat, Jiri,et al."Application of 96-well plate SPE method for analysis of persistent organic pollutants in low volume blood serum samples".CHEMOSPHERE 287(2022).