干旱区生态环境知识资源中心(Arid): Genetic diversity and comparative study of genomic DNA extraction protocols in Tamarix L. species

Arid

DOI	10.36253/caryologia-1056
	Genetic diversity and comparative study of genomic DNA extraction protocols in Tamarix L. species
	Cheng, Xiao; Hong, Xiaoling; Khayatnezhad, Majid; Ullah, Fazal
通讯作者	Cheng, X (corresponding author), Jiangxi Univ Appl Sci, Nanchang 330100, Jiangxi, Peoples R China.
来源期刊	CARYOLOGIA
ISSN	0008-7114
EISSN	2165-5391
出版年	2021
卷号	74 期号:2 页码:131-139
英文摘要	The genus Tamarix consists of about 54 species that mainly grow in saline areas of deserts and semi-deserts. This genus is chemically characterized by the presence of tannins, flavonoids, anthocyanins and essential oils which interfere with the extraction of pure genomic DNA. Thus it is necessary to optimize extraction protocols to minimize the influence of these compounds to the lowest level. The present study compares the efficiency of five different approaches to extract total genomic DNA in Tamarix species, showing significant differences in the extracted DNA contents and quality,by using Kit (DNP (TM) Kit), CfAB DNA extraction method by Murray and Thompson, Sahu et a., Nalini et al. and Bi et a., for the extraction of DNA from Tamarix species. Our results showed significant differences in DNA contents between these five methods. The quantity and quality of extracted genomic DNA were checked by the spectrophotometer, Nano-Drop and and agarose gel electrophoresis analysis. Finally, a PCR-based method was also applied to verify the amplification efficiency for two molecular markers (ITS and ISSR).. In the present study, the genetic diversity of 96 Tamarix individuals species and 8 populations were studied using 10 ISSR markerswhile for nrDNA ITS 8 species samples were used. 'Ihe method of Nalini et al., provided best results (207 ng/mu L) in terms of quantity and quality ofDNA. Our results proposed that this method could be effective for plants with the same polysaccharides, proteins and polyphenols components. The advantage of this method is simple and fast as it does not involve time consuming steps such as incubation at higher temperatures, and also do not requires expensive chemicals such as proteinase K, liquid nitrogen.,. The success of this method in obtaining high-quality genomic DNA has been demonstrated in the Tamarix species group and the reliability of this method has been discussed.
英文关键词	DNA yield extraction protocols Tamarix ISSR secondary metabolites
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000707723400013
WOS关键词	RAPID ISOLATION ; RIBOSOMAL DNA ; PLANT DNA ; AMPLIFICATION ; NILOTICA ; REGIONS ; QUALITY ; MARKERS
WOS类目	Plant Sciences ; Genetics & Heredity
WOS研究方向	Plant Sciences ; Genetics & Heredity
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/362782
作者单位	[Cheng, Xiao; Hong, Xiaoling] Jiangxi Univ Appl Sci, Nanchang 330100, Jiangxi, Peoples R China; [Khayatnezhad, Majid] Islamic Azad Univ, Dept Environm Sci & Engn, Ardabil Branch, Ardebil, Iran; [Ullah, Fazal] Chinese Acad Sci, Chengdu Inst Biol, CAS Key Lab Mt Ecol Restorat & Bioresource Utiliz, Biodivers Conservat Key Lab Sichuan Prov, POB 416, Chengdu 610041, Sichuan, Peoples R China; [Ullah, Fazal] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Cheng, Xiao,Hong, Xiaoling,Khayatnezhad, Majid,et al. Genetic diversity and comparative study of genomic DNA extraction protocols in Tamarix L. species[J],2021,74(2):131-139.
APA	Cheng, Xiao,Hong, Xiaoling,Khayatnezhad, Majid,&Ullah, Fazal.(2021).Genetic diversity and comparative study of genomic DNA extraction protocols in Tamarix L. species.CARYOLOGIA,74(2),131-139.
MLA	Cheng, Xiao,et al."Genetic diversity and comparative study of genomic DNA extraction protocols in Tamarix L. species".CARYOLOGIA 74.2(2021):131-139.