干旱区生态环境知识资源中心(Arid): Photosynthetic contribution and characteristics of cucumber stems and petioles

Arid

DOI	10.1186/s12870-021-03233-w
	Photosynthetic contribution and characteristics of cucumber stems and petioles
	Sun, Weike; Ma, Ning; Huang, Hongyu; Wei, Jingwei; Ma, Si; Liu, Huan; Zhang, Shi; Zhang, Zhenxian; Sui, Xiaolei; Li, Xin
通讯作者	Li, X (corresponding author), China Agr Univ, Beijing Key Lab Growth & Dev Regulat Protected Ve, Dept Vegetable Sci, Coll Hort, Yuanmingyuan Xilu 2, Beijing 100193, Peoples R China.
来源期刊	BMC PLANT BIOLOGY
ISSN	1471-2229
出版年	2021
卷号	21 期号:1
英文摘要	Background: Photosynthesis in the green leafless blade tissues or organs of plants has been studied in some plants, but the photosynthetic characteristics of stems and petioles are poorly understood. Cucurbitaceous plants are climbing plants that have substantial stem and petiole biomass. Understanding the photosynthetic contribution of cucumber stems and petioles to their growth and the underlying molecular mechanisms are important for the regulating of growth in cucumber production. Results: In this study, the photosynthetic capacity of cucumber stems and petioles were determined by (CO2)-C-14 uptake. The total carbon fixed by the stems and petioles was approximately 4% of that fixed by one leaf blade in the cucumber seedling stage, while the proportion of the carbon accumulated in the stems and petioles that redistributed to sink organs (roots and shoot apexes) obviously increased under leafless conditions. The photosynthetic properties of cucumber stems and petioles were studied using a combination of electron microscopy and isotope tracers to compare these properties of stems and petioles with those of leaf blade using two genotypes of cucumber (dark green and light green). Compared with those of the leaf blades, the chlorophyll contents of the cucumber stems and petioles were lower, and the stems and petioles had lower chloroplast numbers and lower stoma numbers but higher thylakoid grana lamella numbers and larger stoma sizes. The Chl a/b ratios were also decreased in the petioles and stems compared with those in the leaf blades. The total photosynthetic rates of the stems and petioles were equivalent to 6 similar to 8% of that of one leaf blade, but the respiration rates were similar in all the three organs, with an almost net 0 photosynthetic rate in the stems and petioles. Transcriptome analysis showed that compared with the leaf blades, the stems and petioles has significantly different gene expression levels in photosynthesis, porphyrin and chlorophyll metabolism; photosynthetic antenna proteins; and carbon fixation. PEPC enzyme activities were higher in the stems and petioles than in the leaf blades, suggesting that the photosynthetic and respiratory mechanisms in stems and petioles are different from those in leaf blade, and these results are consistent with the gene expression data. Conclusions: In this study, we confirmed the photosynthetic contribution to the growth of cucumber stems and petioles, and showed their similar photosynthetic patterns in the terms of anatomy, molecular biology and physiology, which were different from those of cucumber leaf blades.
英文关键词	Cucumber Stem Petiole C-14 Photosynthesis
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000704797300007
WOS关键词	CARBON-DIOXIDE ; DESERT ; LEAF ; LIGHT ; CHLOROPLASTS ; EXCHANGE ; BEHAVIOR ; SPINACH ; GROWTH ; LEAVES
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
来源机构	中国农业大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/362725
作者单位	[Sun, Weike; Ma, Ning; Wei, Jingwei; Ma, Si; Liu, Huan; Zhang, Shi; Zhang, Zhenxian; Sui, Xiaolei; Li, Xin] China Agr Univ, Beijing Key Lab Growth & Dev Regulat Protected Ve, Dept Vegetable Sci, Coll Hort, Yuanmingyuan Xilu 2, Beijing 100193, Peoples R China; [Huang, Hongyu] Tianjin Kernel Cucumber Res Inst, State Key Lab Vegetable Germplasm Innovat, 301 Baidi Rd, Tianjin 300192, Peoples R China
推荐引用方式 GB/T 7714	Sun, Weike,Ma, Ning,Huang, Hongyu,et al. Photosynthetic contribution and characteristics of cucumber stems and petioles[J]. 中国农业大学,2021,21(1).
APA	Sun, Weike.,Ma, Ning.,Huang, Hongyu.,Wei, Jingwei.,Ma, Si.,...&Li, Xin.(2021).Photosynthetic contribution and characteristics of cucumber stems and petioles.BMC PLANT BIOLOGY,21(1).
MLA	Sun, Weike,et al."Photosynthetic contribution and characteristics of cucumber stems and petioles".BMC PLANT BIOLOGY 21.1(2021).