干旱区生态环境知识资源中心(Arid): Whole-genome sequencing of two Streptomyces strains isolated from the sand dunes of Sahara

Arid

DOI	10.1186/s12864-021-07866-x
	Whole-genome sequencing of two Streptomyces strains isolated from the sand dunes of Sahara
	Zerouki, Chahira; Bensalah, Farid; Kuittinen, Suvi; Pappinen, Ari; Turunen, Ossi
通讯作者	Zerouki, C (corresponding author), Univ Eastern Finland, Sch Forest Sci, FI-80101 Joensuu, Finland. ; Zerouki, C (corresponding author), Univ ORAN 1, Dept Biol, Lab Microbial Genet, Oran 31000, Algeria.
来源期刊	BMC GENOMICS
ISSN	1471-2164
出版年	2021
卷号	22 期号:1
英文摘要	BackgroundSahara is one of the largest deserts in the world. The harsh climatic conditions, especially high temperature and aridity lead to unique adaptation of organisms, which could be a potential source of new metabolites. In this respect, two Saharan soils from El Oued Souf and Beni Abbes in Algeria were collected. The bacterial isolates were selected by screening for antibacterial, antifungal, and enzymatic activities. The whole genomes of the two native Saharan strains were sequenced to study desert Streptomyces microbiology and ecology from a genomic perspective.ResultsStrains Babs14 (from Beni Abbes, Algeria) and Osf17 (from El Oued Souf, Algeria) were initially identified by 16S rRNA sequencing as belonging to the Streptomyces genus. The whole genome sequencing of the two strains was performed using Pacific Biosciences Sequel II technology (PacBio), which showed that Babs14 and Osf17 have a linear chromosome of 8.00Mb and 7.97Mb, respectively. The number of identified protein coding genes was 6910 in Babs14 and 6894 in Osf17. No plasmids were found in Babs14, whereas three plasmids were detected in Osf17. Although the strains have different phenotypes and are from different regions, they showed very high similarities at the DNA level. The two strains are more similar to each other than either is to the closest database strain. The search for potential secondary metabolites was performed using antiSMASH and predicted 29 biosynthetic gene clusters (BGCs). Several BGCs and proteins were related to the biosynthesis of factors needed in response to environmental stress in temperature, UV light and osmolarity.ConclusionThe genome sequencing of Saharan Streptomyces strains revealed factors that are related to their adaptation to an extreme environment and stress conditions. The genome information provides tools to study ecological adaptation in a desert environment and to explore the bioactive compounds of these microorganisms. The two whole genome sequences are among the first to be sequenced for the Streptomyces genus of Algerian Sahara. The present research was undertaken as a first step to more profoundly explore the desert microbiome.
英文关键词	Sahara Soil Streptomyces Complete genome sequence Secondary metabolites Enzymes
类型	Article
语种	英语
开放获取类型	gold, Green Published, Green Submitted
收录类别	SCI-E
WOS记录号	WOS:000679094400005
WOS关键词	BACILLUS-SUBTILIS ; ISLANDS ; STRESS ; BACTERIA ; VERSION ; SERVER ; GENES ; SOILS
WOS类目	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/362721
作者单位	[Zerouki, Chahira; Kuittinen, Suvi; Pappinen, Ari; Turunen, Ossi] Univ Eastern Finland, Sch Forest Sci, FI-80101 Joensuu, Finland; [Zerouki, Chahira; Bensalah, Farid] Univ ORAN 1, Dept Biol, Lab Microbial Genet, Oran 31000, Algeria
推荐引用方式 GB/T 7714	Zerouki, Chahira,Bensalah, Farid,Kuittinen, Suvi,et al. Whole-genome sequencing of two Streptomyces strains isolated from the sand dunes of Sahara[J],2021,22(1).
APA	Zerouki, Chahira,Bensalah, Farid,Kuittinen, Suvi,Pappinen, Ari,&Turunen, Ossi.(2021).Whole-genome sequencing of two Streptomyces strains isolated from the sand dunes of Sahara.BMC GENOMICS,22(1).
MLA	Zerouki, Chahira,et al."Whole-genome sequencing of two Streptomyces strains isolated from the sand dunes of Sahara".BMC GENOMICS 22.1(2021).