干旱区生态环境知识资源中心(Arid): Comprehensive genome analysis of a novel actinobacterium with high potential for biotechnological applications, Nonomuraea aridisoli sp. nov., isolated from desert soil

Arid

DOI	10.1007/s10482-021-01654-z
	Comprehensive genome analysis of a novel actinobacterium with high potential for biotechnological applications, Nonomuraea aridisoli sp. nov., isolated from desert soil
	Saygin, Hayrettin; Ay, Hilal; Guven, Kiymet; Cetin, Demet; Sahin, Nevzat
通讯作者	Sahin, N (corresponding author), Ondokuz Mayis Univ, Fac Sci & Arts, Dept Mol Biol & Genet, TR-55139 Samsun, Turkey.
来源期刊	ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
ISSN	0003-6072
EISSN	1572-9699
出版年	2021
英文摘要	During a study to isolate such actinobacteria with unique metabolic potential, a novel actinobacterium, designated KC333(T), was isolated from a soil sample collected from the Karakum Desert, Turkmenistan. The taxonomic position of the strain was investigated using a polyphasic approach. Phylogenetic analysis of the 16S rRNA gene sequence showed that the strain was most closely related to Nonomuraea terrae CH32(T) (99.0% sequence similarity), Nonomuraea maritima FXJ7.203( T) (98.9%), Nonomuraea candida HMC10(T) (98.7%) and Nonomuraea gerenzanensis ATCC 39727( T) (98.6%), and is therefore considered to represent a member of the genus Nonomuraea. However, the average nucleotide identity and digital DNA-DNA hybridization based on whole-genome sequences between strain KC333(T) and close relatives demonstrated that it represents a novel species of the genus Nonomuraea. The major cellular fatty acids of strain KC333(T) were iso-C-16: 0, C-17:0 10-methyl and iso-C-16: 0 2OH. Strain KC333(T) contained meso-diaminopimelic, mannose, madurose and ribose in the cell-wall peptidoglycan. The predominant menaquinones were MK-9(H-4) and MK-9(H-6). The genome size of strain KC333(T) is approximately 9.86 Mb, and the genomic DNA G + C content of the strain is 71.3%. In addition to the polyphasic characterisation, comprehensive genome analysis for gene clusters encoding carbohydrate-active enzymes and bioactive secondary metabolites as well as CRISPR-associated sequences revealed the high biotechnological potential of the strain. Based on evidence collected from the genotypic, phenotypic, and phylogenetic analyses, a novel species, Nonomuraea aridisoli sp. nov. is proposed with KC333(T) (= DSM 107062( T) = JCM 32584( T) = KCTC 49111( T)) as the type strain.
英文关键词	Actinobacteria Bioactive gene clusters Carbohydrate-active enzymes CRISPR-associated sequences Desert
类型	Article ; Early Access
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000696492400003
WOS关键词	GENUS NONOMURAEA ; ACTINOMYCETE ; RECLASSIFICATION ; CLASSIFICATION ; IMPROVEMENTS ; SEQUENCES ; BACTERIA ; DATABASE ; STRAIN ; TREES
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/362468
作者单位	[Saygin, Hayrettin; Ay, Hilal; Sahin, Nevzat] Ondokuz Mayis Univ, Fac Sci & Arts, Dept Mol Biol & Genet, TR-55139 Samsun, Turkey; [Saygin, Hayrettin] Ondokuz Mayis Univ, Fac Sci & Arts, Dept Biol, TR-55139 Samsun, Turkey; [Guven, Kiymet] Eskisehir Tech Univ, Fac Sci, Dept Biol, TR-26555 Eskisehir, Turkey; [Cetin, Demet] Gazi Univ, Dept Math & Sci Educ, Div Sci Educ, TR-06500 Ankara, Turkey
推荐引用方式 GB/T 7714	Saygin, Hayrettin,Ay, Hilal,Guven, Kiymet,et al. Comprehensive genome analysis of a novel actinobacterium with high potential for biotechnological applications, Nonomuraea aridisoli sp. nov., isolated from desert soil[J],2021.
APA	Saygin, Hayrettin,Ay, Hilal,Guven, Kiymet,Cetin, Demet,&Sahin, Nevzat.(2021).Comprehensive genome analysis of a novel actinobacterium with high potential for biotechnological applications, Nonomuraea aridisoli sp. nov., isolated from desert soil.ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY.
MLA	Saygin, Hayrettin,et al."Comprehensive genome analysis of a novel actinobacterium with high potential for biotechnological applications, Nonomuraea aridisoli sp. nov., isolated from desert soil".ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY (2021).