干旱区生态环境知识资源中心(Arid): Analysis of Carotenoids in Haloarchaea Species from Atacama Saline Lakes by High Resolution UHPLC-Q-Orbitrap-Mass Spectrometry: Antioxidant Potential and Biological Effect on Cell Viability

Arid

DOI	10.3390/antiox10081230
	Analysis of Carotenoids in Haloarchaea Species from Atacama Saline Lakes by High Resolution UHPLC-Q-Orbitrap-Mass Spectrometry: Antioxidant Potential and Biological Effect on Cell Viability
	Lizama, Catherine; Romero-Parra, Javier; Andrade, Daniel; Riveros, Felipe; Borquez, Jorge; Ahmed, Shakeel; Venegas-Salas, Luis; Cabalin, Carolina; Simirgiotis, Mario J.
通讯作者	Simirgiotis, MJ (corresponding author), Univ Austral Chile, Fac Ciencias, Inst Farm, Valdivia 5090000, Chile.
来源期刊	ANTIOXIDANTS
EISSN	2076-3921
出版年	2021
卷号	10 期号:8
英文摘要	Haloarchaea are extreme halophilic microorganisms belonging to the domain Archaea, phylum Euryarchaeota, and are producers of interesting antioxidant carotenoid compounds. In this study, four new strains of Haloarcula sp., isolated from saline lakes of the Atacama Desert, are reported and studied by high-resolution mass spectrometry (UHPLC-Q-Orbitrap-MS/MS) for the first time. In addition, determination of the carotenoid pigment profile from the new strains of Haloarcula sp., plus two strains of Halorubrum tebenquichense, and their antioxidant activity by means of several methods is reported. The effect of biomass on cellular viability in skin cell lines was also evaluated by MTT assay. The cholinesterase inhibition capacity of six haloarchaea (Haloarcula sp. ALT-23; Haloarcula sp. TeSe-41; Haloarcula sp. TeSe-51; Haloarcula sp. Te Se-89 and Halorubrum tebenquichense strains TeSe-85 and Te Se-86) is also reported for the first time. AChE inhibition IC50 was 2.96 +/- 0.08 mu g/mL and BuChE inhibition IC50 was 2.39 +/- 0.09 mu g/mL for the most active strain, Halorubrum tebenquichense Te Se-85, respectively, which is more active in BuCHe than that of the standard galantamine. Docking calculation showed that carotenoids can exert their inhibitory activity fitting into the enzyme pocket by their halves, in the presence of cholinesterase dimers.
英文关键词	UHPLC Orbitrap high resolution MS analysis haloarchaea bacterioruberin carotenoids cell viability in HaCaT Atacama saline lake docking studies
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000688711300001
WOS关键词	HALOPHILIC ARCHAEA ; HUMAN BUTYRYLCHOLINESTERASE ; IN-VITRO ; ACETYLCHOLINESTERASE ; BACTERIORUBERIN ; COMPLEXES ; PLANTS
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Medicinal ; Food Science & Technology
WOS研究方向	Biochemistry & Molecular Biology ; Pharmacology & Pharmacy ; Food Science & Technology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/362466
作者单位	[Lizama, Catherine; Andrade, Daniel; Riveros, Felipe] Univ Antofagasta, Fac Ciencias Salud, Dept Tecnol Med, Antofagasta 1240000, Chile; [Romero-Parra, Javier] Univ Chile, Fac Ciencias Quim & Farmaceut, Dept Quim Organ & Fisicoquim, Olivos 1007,Casilla 233, Santiago 6640022, Chile; [Borquez, Jorge] Univ Antofagasta, Fac Ciencias Basicas, Dept Quim, Antofagasta 1240000, Chile; [Ahmed, Shakeel; Simirgiotis, Mario J.] Univ Austral Chile, Fac Ciencias, Inst Farm, Valdivia 5090000, Chile; [Venegas-Salas, Luis; Cabalin, Carolina] Pontificia Univ Catolica Chile, Dept Enfermedades Infecciosas & Inmunol Pediat, Escuela Med, Santiago 8320000, Chile
推荐引用方式 GB/T 7714	Lizama, Catherine,Romero-Parra, Javier,Andrade, Daniel,et al. Analysis of Carotenoids in Haloarchaea Species from Atacama Saline Lakes by High Resolution UHPLC-Q-Orbitrap-Mass Spectrometry: Antioxidant Potential and Biological Effect on Cell Viability[J],2021,10(8).
APA	Lizama, Catherine.,Romero-Parra, Javier.,Andrade, Daniel.,Riveros, Felipe.,Borquez, Jorge.,...&Simirgiotis, Mario J..(2021).Analysis of Carotenoids in Haloarchaea Species from Atacama Saline Lakes by High Resolution UHPLC-Q-Orbitrap-Mass Spectrometry: Antioxidant Potential and Biological Effect on Cell Viability.ANTIOXIDANTS,10(8).
MLA	Lizama, Catherine,et al."Analysis of Carotenoids in Haloarchaea Species from Atacama Saline Lakes by High Resolution UHPLC-Q-Orbitrap-Mass Spectrometry: Antioxidant Potential and Biological Effect on Cell Viability".ANTIOXIDANTS 10.8(2021).