干旱区生态环境知识资源中心(Arid): Molecular Detection, Serotyping, and Antibiotic Resistance of Shiga Toxigenic Escherichia coli Isolated from She-Camels and In-Contact Humans in Egypt

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DOI	10.3390/antibiotics10081021
	Molecular Detection, Serotyping, and Antibiotic Resistance of Shiga Toxigenic Escherichia coli Isolated from She-Camels and In-Contact Humans in Egypt
	Diab, Mohamed Said; Tarabees, Reda; Elnaker, Yasser F.; Hadad, Ghada A.; Saad, Marwa A.; Galbat, Salah A.; Albogami, Sarah; Hassan, Aziza M.; Dawood, Mahmoud A. O.; Shaaban, Sabah Ibrahim
通讯作者	Dawood, MAO (corresponding author), Kafrelsheikh Univ, Fac Agr, Dept Anim Prod, Kafrelsheikh 33512, Egypt. ; Dawood, MAO (corresponding author), Amer Univ Cairo, Ctr Appl Res Environm & Sustainabil, Cairo 11835, Egypt.
来源期刊	ANTIBIOTICS-BASEL
ISSN	2079-6382
出版年	2021
卷号	10 期号:8
英文摘要	This study aims to determine the prevalence of STEC in she-camels suffering from mastitis in semi-arid regions by using traditional culture methods and then confirming it with Serological and molecular techniques in milk samples, camel feces, as well as human stool samples for human contacts. In addition, an antibiotic susceptibility profile for these isolates was investigation. Mastitic milk samples were taken after California Mastitis Test (CMT) procedure, and fecal samples were taken from she-camels and human stool samples, then cultured using traditional methods to isolate Escherichia coli. These isolates were initially classified serologically, then an mPCR (Multiplex PCR) was used to determine virulence genes. Finally, both camel and human isolates were tested for antibiotic susceptibility. Out of a total of 180 she-camels, 34 (18.9%) were mastitic (8.3% clinical and 10.6% sub-clinical mastitis), where it was higher in camels bred with other animals. The total presence of E. coli was 21.9, 13.9, and 33.7% in milk, camel feces, and human stool, respectively, whereas the occurrence of STEC from the total E. coli isolates were 36, 16, and 31.4% for milk, camel feces, and stool, respectively. Among the camel isolates, stx(1) was the most frequently detected virulence gene, while hlyA was not detected. The most detected virulence gene in human isolates was stx(2) (45.5%), followed by stx(1). Camel STEC showed resistance to Oxytetracycline only, while human STEC showed multiple drug resistance to Amoxicillin, Gentamycin, and Clindamycin with 81.8, 72.7, and 63.6%, respectively. Breeding camels in semi-arid areas separately from other animals may reduce the risk of infection with some bacteria, including E. coli; in contrast, mixed breeding with other animals contributes a significant risk factor for STEC emergence in camels.
英文关键词	antibiotic resistance camel Escherichia coli domestic milk mastitis
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000688615800001
WOS关键词	VIRULENCE GENES ; DAIRY-CATTLE ; PREVALENCE ; MASTITIS ; CALVES ; MILK ; SUSCEPTIBILITY ; DROMEDARIUS ; PATHOGENS ; PROFILES
WOS类目	Infectious Diseases ; Pharmacology & Pharmacy
WOS研究方向	Infectious Diseases ; Pharmacology & Pharmacy
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/362464
作者单位	[Diab, Mohamed Said] New Valley Univ, Fac Vet Med, Dept Anim Hyg & Zoonoses, El Kharga 72511, Egypt; [Tarabees, Reda] Univ Sadat City, Fac Vet Med, Dept Bacteriol Mycol & Immunol, Sadat City 32897, Egypt; [Elnaker, Yasser F.; Galbat, Salah A.] New Valley Univ, Fac Vet Med, Dept Anim Med Infect Dis, El Kharga 72511, Egypt; [Hadad, Ghada A.] Univ Sadat City, Fac Vet Med, Dept Anim Hyg & Zoonoses, Sadat City 32897, Egypt; [Saad, Marwa A.] Menoufia Univ, Fac Vet Med, Dept Food Control, Menoufia 32511, Shebinal Kom, Egypt; [Albogami, Sarah; Hassan, Aziza M.] Taif Univ, Coll Sci, Dept Biotechnol, POB 11099, At Taif 21944, Saudi Arabia; [Dawood, Mahmoud A. O.] Kafrelsheikh Univ, Fac Agr, Dept Anim Prod, Kafrelsheikh 33512, Egypt; [Dawood, Mahmoud A. O.] Amer Univ Cairo, Ctr Appl Res Environm & Sustainabil, Cairo 11835, Egypt; [Shaaban, Sabah Ibrahim] Damanhour Univ, Fac Vet Med, Dept Anim Hyg & Zoonoses, Damanhour 22511, Egypt
推荐引用方式 GB/T 7714	Diab, Mohamed Said,Tarabees, Reda,Elnaker, Yasser F.,et al. Molecular Detection, Serotyping, and Antibiotic Resistance of Shiga Toxigenic Escherichia coli Isolated from She-Camels and In-Contact Humans in Egypt[J],2021,10(8).
APA	Diab, Mohamed Said.,Tarabees, Reda.,Elnaker, Yasser F..,Hadad, Ghada A..,Saad, Marwa A..,...&Shaaban, Sabah Ibrahim.(2021).Molecular Detection, Serotyping, and Antibiotic Resistance of Shiga Toxigenic Escherichia coli Isolated from She-Camels and In-Contact Humans in Egypt.ANTIBIOTICS-BASEL,10(8).
MLA	Diab, Mohamed Said,et al."Molecular Detection, Serotyping, and Antibiotic Resistance of Shiga Toxigenic Escherichia coli Isolated from She-Camels and In-Contact Humans in Egypt".ANTIBIOTICS-BASEL 10.8(2021).