干旱区生态环境知识资源中心(Arid): Automatic and renewable micro-solid-phase extraction based on bead injection lab-on-valve system for determination of tranexamic acid in urine by UHPLC coupled with tandem mass spectrometry

Arid

DOI	10.1007/s00216-021-03606-y
	Automatic and renewable micro-solid-phase extraction based on bead injection lab-on-valve system for determination of tranexamic acid in urine by UHPLC coupled with tandem mass spectrometry
	Fernandes, Sara R.; Barreiros, Luisa; Sa, Paula; Miro, Manuel; Segundo, Marcela A.
通讯作者	Segundo, MA (corresponding author), Univ Porto, Fac Farm, Dept Ciencias Quim, REQUIMTE,LAQV, Rua Jorge Viterbo Ferreira 228, P-4050313 Porto, Portugal.
来源期刊	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
ISSN	1618-2642
EISSN	1618-2650
出版年	2021-08
英文摘要	An automatic micro-solid-phase extraction (mu SPE) method using on-line renewable sorbent beads followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was established for the determination of tranexamic acid (TXA) in urine. The mu SPE method was based on the bead injection (BI) concept combined with the mesofluidic lab-on-valve (LOV) platform. All steps of the mu SPE-BI-LOV were implemented by computer programming, rendering enhanced precision on time and flow events. Several parameters, including the type of sorbent, volume and composition of the conditioning solution, washing solution, and eluent composition, were evaluated to improve the extraction efficiency. The best results were obtained with a hydrophilic-lipophilic balanced mixed-mode sorbent, decorated with sulfonic acid groups (Oasis MCX), and 99% acetonitrile-water (50:50, v/v)-1% ammonium hydroxide as eluent. Chromatographic separation was performed using a BEH amide column coupled to MS/MS detection in positive ionization mode. Good linearity was achieved (R-2 > 0.998) for TXA concentrations in urine ranging from 300 to 3000 ng mL(-1), with LOD and LOQ of 30 and 65 ng mL(-1), respectively. Dilution integrity was observed for dilution factors up to 20,000 times, providing the extension of the upper limit of quantification to 12 mg mL(-1). The method was validated according to international guidelines and successfully applied to urine samples collected during scoliosis surgery of pediatric patients treated with TXA.
英文关键词	Bead injection Lab-on-valve Automation Mass spectrometry Tranexamic acid Urine
类型	Article ; Early Access
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000686505600001
WOS关键词	LIQUID-CHROMATOGRAPHY ; SAMPLE PREPARATION ; PLASMA
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/362448
作者单位	[Fernandes, Sara R.; Barreiros, Luisa; Segundo, Marcela A.] Univ Porto, Fac Farm, Dept Ciencias Quim, REQUIMTE,LAQV, Rua Jorge Viterbo Ferreira 228, P-4050313 Porto, Portugal; [Fernandes, Sara R.; Barreiros, Luisa] Inst Politecn Porto, Escola Super Saude, Rua Dr Antonio Bernardino de Almeida 400, P-4200072 Porto, Portugal; [Sa, Paula] Ctr Hosp Univ Porto, P-4099001 Porto, Portugal; [Miro, Manuel] Univ Balearic Isl, Dept Chem, FI TRACE Grp, E-07122 Palma De Mallorca, Spain
推荐引用方式 GB/T 7714	Fernandes, Sara R.,Barreiros, Luisa,Sa, Paula,et al. Automatic and renewable micro-solid-phase extraction based on bead injection lab-on-valve system for determination of tranexamic acid in urine by UHPLC coupled with tandem mass spectrometry[J],2021.
APA	Fernandes, Sara R.,Barreiros, Luisa,Sa, Paula,Miro, Manuel,&Segundo, Marcela A..(2021).Automatic and renewable micro-solid-phase extraction based on bead injection lab-on-valve system for determination of tranexamic acid in urine by UHPLC coupled with tandem mass spectrometry.ANALYTICAL AND BIOANALYTICAL CHEMISTRY.
MLA	Fernandes, Sara R.,et al."Automatic and renewable micro-solid-phase extraction based on bead injection lab-on-valve system for determination of tranexamic acid in urine by UHPLC coupled with tandem mass spectrometry".ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2021).