干旱区生态环境知识资源中心(Arid): Lignocelluloytic activities and composition of bacterial community in the camel rumen

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DOI	10.3934/microbiol.2021022
	Lignocelluloytic activities and composition of bacterial community in the camel rumen
	Rabee, Alaa Emara; Forster, Robert; Sabra, Ebrahim A.
通讯作者	Rabee, AE (corresponding author), Desert Res Ctr, Anim & Poultry Nutr Dept, Cairo, Egypt.
来源期刊	AIMS MICROBIOLOGY
ISSN	2471-1888
出版年	2021
卷号	7 期号:3 页码:354-367
英文摘要	The camel is well-adapted to utilize the poor-quality forages in the harsh desert conditions as the camel rumen sustains fibrolytic microorganisms, mainly bacteria that are capable of breaking down the lignocellulosic biomass efficiently. Exploring the composition of the bacterial community in the rumen of the camel and quantifying their cellulolytic and xylanolytic activities could lead to understanding and improving fiber fermentation and discovering novel sources of cellulases and xylanases. In this study, Illumina MiSeq sequencing of the V4 region on 16S rRNA was applied to identify the bacterial and archaeal communities in the rumen of three camels fed wheat straw and broom corn. Furthermore, rumen samples were inoculated into bacterial media enriched with xylan and different cellulose sources, including filter paper (FP), wheat straw (WS), and alfalfa hay (AH) to assess the ability of rumen bacteria to produce endo-cellulase and endo-xylanase at different fermentation intervals. The results revealed that the phylum Bacteroidetes dominated the bacterial community and Candidatus Methanomethylophilus dominated the archaeal community. Also, most of the bacterial community has fibrolytic potential and the dominant bacterial genera were Prevotella, RC9_gut_group, Butyrivibrio, Ruminococcus, Fibrobacteres, and Treponema. The highest xylanase production (884.8 mU/mL) was observed at 7 days. The highest cellulase production (1049.5 mU/mL) was observed when rumen samples were incubated with Alfalfa hay for 7 days.
英文关键词	Camel rumen bacteria archaea enzymes cellulase xylanase
类型	Article
语种	英语
开放获取类型	gold
收录类别	ESCI
WOS记录号	WOS:000707446400005
WOS关键词	BUFFALO BUBALUS-BUBALIS ; DIVERSITY ; ENZYMES ; CELLULASE ; OPTIMIZATION ; FORESTOMACH ; FOREGUT ; PHYLUM ; FORAGE ; SUGAR
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/362430
作者单位	[Rabee, Alaa Emara] Desert Res Ctr, Anim & Poultry Nutr Dept, Cairo, Egypt; [Forster, Robert] Agr & Agri Food Canada, Lethbridge Res & Dev Ctr, Lethbridge, AB, Canada; [Sabra, Ebrahim A.] Univ Sadat City, Genet Engn & Biotechnol Res Inst, Anim Biotechnol Dept, Sadat City, Egypt
推荐引用方式 GB/T 7714	Rabee, Alaa Emara,Forster, Robert,Sabra, Ebrahim A.. Lignocelluloytic activities and composition of bacterial community in the camel rumen[J],2021,7(3):354-367.
APA	Rabee, Alaa Emara,Forster, Robert,&Sabra, Ebrahim A..(2021).Lignocelluloytic activities and composition of bacterial community in the camel rumen.AIMS MICROBIOLOGY,7(3),354-367.
MLA	Rabee, Alaa Emara,et al."Lignocelluloytic activities and composition of bacterial community in the camel rumen".AIMS MICROBIOLOGY 7.3(2021):354-367.