干旱区生态环境知识资源中心(Arid): Improved Conservation of Coffee (Coffea arabica L.) Germplasm via Micropropagation and Cryopreservation

Arid

DOI	10.3390/agronomy11091861
	Improved Conservation of Coffee (Coffea arabica L.) Germplasm via Micropropagation and Cryopreservation
	Castilla Valdes, Yanelis; Shukla, Mukund R.; Gonzalez Vega, Maria Esther; Saxena, Praveen K.
通讯作者	Saxena, PK (corresponding author), Univ Guelph, Gosling Res Inst Plant Preservat, Dept Plant Agr, Guelph, ON N1G 2W1, Canada.
来源期刊	AGRONOMY-BASEL
EISSN	2073-4395
出版年	2021
卷号	11 期号:9
英文摘要	Coffee (Coffea spp.) is an important tropical agricultural crop that has significant economic and social importance in the world. The ex situ conservation of plant genetic resources through seeds is not feasible due to the sensitivity of coffee seed to desiccation and low temperatures. The cryopreservation of zygotic embryos may allow for an efficient and long-term storage of coffee germplasm. This study describes the cryopreservation methods for conserving zygotic embryos of Coffea arabica L. for the long-term conservation of currently available germplasm. Zygotic embryos were successfully cryopreserved in liquid nitrogen at -196 degrees C under controlled environmental conditions with either droplet-vitrification or encapsulation-vitrification protocols without dehydration. Zygotic embryos had the highest regrowth (100%) following droplet-vitrification cryopreservation using the Plant Vitrification Solution 3 (PVS3) for 40 min at 23 degrees C. In the case of encapsulation-vitrification using PVS3 for 40 min at 23 degrees C, the embryo regeneration response was 78%. Plantlets were recovered following shoot multiplication using a temporary immersion system (TIS) and in vitro rooting. The prolific rooting of shoots was observed after 4 weeks of culture in the liquid medium with plugs made of the inert substrate Oasis (R) in vitro Express (IVE) compared to the semi-solid medium. The successful cryopreservation of coffee zygotic embryos using droplet vitrification and encapsulation-vitrification followed by micropropagation in temporary immersion culture system has not been reported earlier and together these technologies are anticipated to further facilitate the initiatives for the conservation and distribution of coffee germplasm.
英文关键词	coffee seed zygotic embryo cryopreservation droplet vitrification encapsulation-vitrification micropropagation rocker culture system acclimatization
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:000699318100001
WOS关键词	IN-VITRO PLANTLETS ; SHOOT TIPS ; ZYGOTIC EMBRYOS ; ENCAPSULATION-DEHYDRATION ; DROPLET-VITRIFICATION ; SOMATIC EMBRYOS ; REGENERATION ; ANTIOXIDANT ; BIOREACTOR ; REGROWTH
WOS类目	Agronomy ; Plant Sciences
WOS研究方向	Agriculture ; Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/362425
作者单位	[Castilla Valdes, Yanelis; Shukla, Mukund R.; Saxena, Praveen K.] Univ Guelph, Gosling Res Inst Plant Preservat, Dept Plant Agr, Guelph, ON N1G 2W1, Canada; [Castilla Valdes, Yanelis; Gonzalez Vega, Maria Esther] Natl Inst Agr Sci INCA, Dept Genet & Plant Breeding, Carretera Tapaste,Km 3 1-2, San Jose De Las Lajas 32700, Cuba
推荐引用方式 GB/T 7714	Castilla Valdes, Yanelis,Shukla, Mukund R.,Gonzalez Vega, Maria Esther,et al. Improved Conservation of Coffee (Coffea arabica L.) Germplasm via Micropropagation and Cryopreservation[J],2021,11(9).
APA	Castilla Valdes, Yanelis,Shukla, Mukund R.,Gonzalez Vega, Maria Esther,&Saxena, Praveen K..(2021).Improved Conservation of Coffee (Coffea arabica L.) Germplasm via Micropropagation and Cryopreservation.AGRONOMY-BASEL,11(9).
MLA	Castilla Valdes, Yanelis,et al."Improved Conservation of Coffee (Coffea arabica L.) Germplasm via Micropropagation and Cryopreservation".AGRONOMY-BASEL 11.9(2021).