干旱区生态环境知识资源中心(Arid): 黑果枸杞不同组织内生细菌群落多样性

Arid

	黑果枸杞不同组织内生细菌群落多样性
其他题名	Diversity and function analysis of endophytic bacterial community in different tissues of Lycium ruthenicum Murr
	顾美英; 古丽尼沙·沙依木; 张志东; 朱静; 刘晓静; 唐琦勇; 欧提库尔·玛合水提; 宋素琴; 冯雷; 唐光木; 徐万里
来源期刊	微生物学报
ISSN	0001-6209
出版年	2021
卷号	61 期号:1 页码:152-166
中文摘要	【目的】黑果枸杞是我国荒漠区特有的药用盐生植物,本研究分析了黑果枸杞不同组织中内生细菌群落多样性特征及分布规律。【方法】应用Illumina MiSeq高通量测序技术对黑果枸杞内生细菌的16S rRNA V5-V7区域序列进行测定,并分析群落组成、多样性及功能等生物学信息。【结果】黑果枸杞不同组织内生细菌群落多样性及功能均有较大的差异。花、叶、果、茎和根产生的OTUs分别是182、173、 119、187和254,群落多样性表现为根>花>果、茎>叶。从门水平上看,变形菌门是优势菌门,在不同组织中均有分布,花、叶、果、茎和根中的相对丰度分别为87.66%、41.51%、81.76%、97.67%和61.85%。在属水平上显示内生细菌的分布表现出器官差异性。花部能够准确分类的优势菌属为沙雷氏菌属和不动杆菌属,相对丰度分别为11.57%和8.55%。叶部为红球菌属和慢生根瘤菌属,相对丰度分别为29.68%和5.53%。果实中为泛菌属、红球菌属和沙雷氏菌属,相对丰度分别为23.12%、5.52%和4.29%。茎部为沙雷氏菌属和假单胞菌属,相对丰度分别为12.03% 和17.71%。根部为盐单胞菌属、Fodinicurvata和Lipingzhangella,相对丰度分别为24.18%、5.16%和4.86% 。在不同组织中分布较广的盐单胞菌、沙雷氏菌、不动杆菌、红球菌、泛菌等菌属均具有较高耐盐性和促生、生防、降解有机污染物及抗氧化等功能。PICRUS t功能预测分析显示,黑果枸杞组织中内生细菌功能中涉及丰富的多糖、萜类和酮类、酶及维他命等次生代谢产物的生物合成。【结论】黑果枸杞内生细菌具有丰富的群落和功能多样性,拥有多种益生功能性状,也含有多个与人和植物体代谢相关的功能信息。不同组织优势菌属和功能信息各有不同,其中根部的内生细菌物种最丰富,花部和茎部参与各种代谢调控的细菌丰度最高。此外,不同组织中还含有大量未知种属的微生物类群,这些都为内生细菌功能利用和挖掘新的有益微生物资源提供广阔的发展空间。
英文摘要	[Objective] Lycium ruthenicum Murr. is an important medicinal and halophyte plant of Solanaceae family in desert regions of China. Our study aims to reveal the diversity and distribution of endophytic bacterial community in different tissues of Lycium ruthenicum Murr.. [Methods] Through high-throughput sequencing technology, sequences at V5-V7 regions of endophytic bacteria 16S rRNA gene in different tissues of Lycium ruthenicum Murr. were amplified, and community composition, diversity and function were analyzed by bioinformatics. [Results] The diversity and function of endophytic bacterial community in different tissues of Lycium ruthenicum Murr. were significantly different. The OTUs (operational taxonomic units) of flower, leaf, fruit, stem and root were 182, 173, 119, 187 and 254, respectively. The community diversity index showed that root > flower > fruit, stem > leaf. At the level of phylum, Proteobacteria was the dominant group, which was simultaneously found in different tissues. The relative abundances of flower, leaf, fruit, stem and root were 87.66%, 41.51%, 81.76%, 97.67% and 61.85%, respectively. At genus level, the distribution of endophytic bacteria displayed difference in different tissues. Serratia and Acinetobacter were the dominant groups in flower, with relative abundances of 11.57% and 8.55%, respectively. Rhodococcus and Bradyrhizobium were the dominant groups in leaf, with relative abundances of 29.68% and 5.53%, respectively. Pantoea,Rhodococcus and Serratia were the dominant groups in fruit, with relative abundances of 23.12%, 5.52% and 4.29%, respectively. Serratia and Pseudomonas were the dominant groups in stem, with relative abundances of 12.03% and 17.71%, respectively. Halomonas, Fodinicurvata and Lipingzhangella were the dominant groups in roots, with relative abundances of 24.18%, 5.16% and 4.86%, respectively. Some dominant microbial groups, such as Halomonas, Serratia, Acinetobacter, Rhodococcus, Pantoea etc., exhibited functional characteristics related to salt tolerance, promoting growth, biological control, degradation of toxic and harmful substances and anti-oxidation etc. PICRUSt function prediction analysis showed that the functions of endophytic bacteria in different tissues were involved in the biosynthesis of secondary metabolites such as polysaccharides, terpenes, ketones, enzymes and vitamins etc. [Conclusion] The endophytic bacteria of Lycium ruthenicum Murr. had rich community and functional diversity. They also contained a variety of probiotics and functional information related to human and plant metabolism. The dominant bacteria genera and functional information of different tissues were different, among which the endophytic bacteria species in the root were the most abundant, and the bacteria involved in various metabolic functions in the flower and stem were the highest. In addition, there were a large number of unknown species and genera in tissues, which provided a broad development space to explore functional utilization of endophytic bacteria and find new microbial resources.
中文关键词	黑果枸杞 ; 不同组织 ; 内生细菌 ; 多样性 ; MiSeq高通量测序 ; 功能预测
英文关键词	Lycium ruthenicum Murr. different tissues endophytic bacteria diversity MiSeq high-throughput sequencing function prediction
类型	Article
语种	中文
收录类别	CSCD
WOS类目	Agriculture
CSCD记录号	CSCD:6888971
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/354483
作者单位	顾美英, 新疆农业科学院微生物应用研究所, 新疆特殊环境微生物实验室, 乌鲁木齐, 新疆 830091, 中国. 古丽尼沙·沙依木, 新疆农业科学院微生物应用研究所, 新疆特殊环境微生物实验室, 乌鲁木齐, 新疆 830091, 中国. 张志东, 新疆农业科学院微生物应用研究所, 新疆特殊环境微生物实验室, 乌鲁木齐, 新疆 830091, 中国. 朱静, 新疆农业科学院微生物应用研究所, 新疆特殊环境微生物实验室, 乌鲁木齐, 新疆 830091, 中国. 刘晓静, 新疆农业科学院微生物应用研究所, 新疆特殊环境微生物实验室, 乌鲁木齐, 新疆 830091, 中国. 唐琦勇, 新疆农业科学院微生物应用研究所, 新疆特殊环境微生物实验室, 乌鲁木齐, 新疆 830091, 中国. 欧提库尔·玛合水提, 新疆农业科学院微生物应用研究所, 新疆特殊环境微生物实验室, 乌鲁木齐, 新疆 830091, 中国. 宋素琴, 新疆农业科学院微生物应用研究所, 新疆特殊环境微生物实验室, 乌鲁木齐, 新疆 830091, 中国. 冯雷, 新疆农业科学院土壤肥料与农业节水研究所, 乌鲁木齐, 新疆 830091, 中国. 唐光木, 新疆农业科学院土壤肥料与农业节水研究所, 乌鲁木齐, 新疆 830091, 中国. 徐万里, 新疆农业科学院土壤肥料与农业节水研究所, 乌鲁木齐, 新疆 830091, 中国.
推荐引用方式 GB/T 7714	顾美英,古丽尼沙·沙依木,张志东,等. 黑果枸杞不同组织内生细菌群落多样性[J],2021,61(1):152-166.
APA	顾美英.,古丽尼沙·沙依木.,张志东.,朱静.,刘晓静.,...&徐万里.(2021).黑果枸杞不同组织内生细菌群落多样性.微生物学报,61(1),152-166.
MLA	顾美英,et al."黑果枸杞不同组织内生细菌群落多样性".微生物学报 61.1(2021):152-166.